FastQCFastQC Report
Thu 26 May 2016
SRR1926928_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926928_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315027
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8810.2796585689480584No Hit
CTGTAGGACGTGGAATATGGCAAGA8730.2771191040767934No Hit
GTATCAACGCAGAGTACTTTTTTTT8340.2647392128293765No Hit
GTCCTAAAGTGTGTATTTCTCATTT8060.25585108577994903No Hit
CTTTAGGACGTGAAATATGGCGAGG7370.2339482012652884No Hit
GTCCTACAGTGTGCATTTCTCATTT6770.21490221473080087No Hit
CTGTAGGACCTGGAATATGGCGAGA5150.16347805108768457No Hit
GGTATCAACGCAGAGTACTTTTTTT5150.16347805108768457No Hit
TATCAACGCAGAGTACTTTTTTTTT4560.14474949766210515No Hit
CTGAAGGACCTGGAATATGGCGAGA4010.12729067667215826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACC250.00594920219.0398274
GTCTAGG453.4333618E-516.9404851
TTCAATA400.00516662514.2912562
CAGTAGT400.005194027414.2798714
CAGTACA500.001471497513.3278794
GGCGAGG2000.013.28765919
GCTACAC603.999127E-412.6952413
CTAGAGT604.0045095E-412.6932194
ACAGTGC751.49112075E-512.6488878
ACTGTTC905.4513293E-712.6488878
TCCTAGA1001.3754106E-712.3857542
GTATAGA550.002989963612.1278471
AGAGGTG550.003011470412.1162545
GTCCTAA1603.6379788E-1211.9112791
ACTGTGC658.087038E-411.6758958
ACTGTCC658.087038E-411.6758958
AGTACAG907.236842E-611.635455
GACGTGG2700.011.6003377
AGGACGT4850.011.5809265
GTCCTAG752.0061123E-411.4348281