##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926928_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 315027 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.808016455732364 31.0 30.0 33.0 25.0 34.0 2 30.079812206572768 31.0 30.0 33.0 26.0 34.0 3 30.174750100785012 31.0 30.0 34.0 26.0 34.0 4 33.757509673773995 35.0 35.0 37.0 28.0 37.0 5 33.710891447399746 37.0 35.0 37.0 28.0 37.0 6 33.69558164855711 37.0 35.0 37.0 29.0 37.0 7 33.68722046046847 37.0 35.0 37.0 29.0 37.0 8 33.705688718744746 37.0 35.0 37.0 29.0 37.0 9 35.227488437499005 39.0 35.0 39.0 29.0 39.0 10 35.096337774222526 39.0 35.0 39.0 29.0 39.0 11 35.14993000599949 39.0 35.0 39.0 29.0 39.0 12 35.0426788814927 39.0 35.0 39.0 29.0 39.0 13 35.059372688690175 39.0 35.0 39.0 29.0 39.0 14 36.12897307214937 40.0 36.0 41.0 28.0 41.0 15 36.13350919127567 40.0 36.0 41.0 29.0 41.0 16 36.11150472816616 40.0 36.0 41.0 28.0 41.0 17 36.07769492773635 40.0 36.0 41.0 28.0 41.0 18 36.06534043113765 40.0 36.0 41.0 28.0 41.0 19 36.141356137727875 40.0 36.0 41.0 28.0 41.0 20 36.12159592669835 40.0 36.0 41.0 28.0 41.0 21 36.048760899859374 40.0 36.0 41.0 27.0 41.0 22 35.96864395750205 40.0 36.0 41.0 27.0 41.0 23 35.892840931094796 40.0 36.0 41.0 27.0 41.0 24 35.82481184152469 40.0 36.0 41.0 27.0 41.0 25 35.6994797271345 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5902184210929917 1101 2 -0.510533098146059 1101 3 -0.2826528705337985 1101 4 -0.7534967317515466 1101 5 -0.9497125366920969 1101 6 -0.8513658372964343 1101 7 -0.593397033919068 1101 8 -0.5868318126153866 1101 9 -0.6976205939695674 1101 10 -0.6135080657206515 1101 11 -0.6311882161824016 1101 12 -0.4705179098743173 1101 13 -0.5213106894807211 1101 14 -0.40469638747462255 1101 15 -0.6097715382345328 1101 16 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0.5953003442517542 2214 13 0.4479078430307126 2214 14 0.17533940492943145 2214 15 0.24200813963383183 2214 16 0.2975538835772795 2214 17 0.375896807577476 2214 18 0.4619907537393928 2214 19 0.2788257825325857 2214 20 0.3933133047050319 2214 21 0.36316352240893934 2214 22 0.228498127181048 2214 23 0.4515515105210852 2214 24 0.3353025938053662 2214 25 0.44798474494701424 2215 1 0.09201403365454652 2215 2 0.3222074544103073 2215 3 0.2680537029816037 2215 4 0.29684464646376085 2215 5 0.33097180754416655 2215 6 0.23204159363402255 2215 7 0.0675667641120512 2215 8 0.18289667261802123 2215 9 0.10448324852011126 2215 10 0.19085839982396635 2215 11 -0.08631682844026045 2215 12 0.14449479257090303 2215 13 0.12237778305023994 2215 14 0.12621818858126943 2215 15 0.2468972455063394 2215 16 0.31170160292447235 2215 17 0.21832601667219365 2215 18 0.0020416673954102293 2215 19 0.16793514227592965 2215 20 0.08263538588789743 2215 21 0.11236843243823103 2215 22 0.06950454369659553 2215 23 -0.004359355708508872 2215 24 0.30136829334504966 2215 25 0.30398627933116984 2216 1 0.13209494027604052 2216 2 0.2746565591800021 2216 3 -2.0168244989636612 2216 4 -1.216098222362362 2216 5 -0.1892511968078523 2216 6 0.11029465318947018 2216 7 0.14281065230319 2216 8 0.0937277909987202 2216 9 0.32776142709884226 2216 10 0.4013397504095906 2216 11 0.3759900325580716 2216 12 0.3960099662134553 2216 13 0.15557651541580952 2216 14 0.002923215306743998 2216 15 0.19133601058505434 2216 16 0.5402226474085126 2216 17 0.21540885103267726 2216 18 0.3539380076121361 2216 19 0.44026843666503623 2216 20 0.6918666269428968 2216 21 0.672807431182278 2216 22 0.7026536221789428 2216 23 0.4727596967785672 2216 24 0.5339930852368653 2216 25 0.31568780107664907 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8037.0 3 4.0 4 123.0 5 815.0 6 1199.0 7 822.0 8 641.0 9 598.0 10 538.0 11 449.0 12 430.0 13 417.0 14 363.0 15 385.0 16 404.0 17 435.0 18 450.0 19 553.0 20 726.0 21 974.0 22 1246.0 23 1493.0 24 1787.0 25 2051.0 26 2540.0 27 2816.0 28 3246.0 29 4065.0 30 5049.0 31 6504.0 32 8597.0 33 11199.0 34 14844.0 35 21235.0 36 31523.0 37 53849.0 38 124620.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.59226583951999 20.46202837384318 10.530865453066205 28.41484033357063 2 13.196779134281119 21.59117630235972 42.26051643162651 22.951528131732644 3 18.00114292970982 26.1251508032256 29.821575973077657 26.05213029398692 4 11.313950867703403 18.492700625660657 39.55978376456621 30.63356474206973 5 11.72685484997397 39.57563521389662 36.46400772034081 12.233502215788604 6 29.621915644386696 39.987388695685404 18.761225971643675 11.629469688284226 7 26.714164158443506 32.37893854535756 23.073748110895213 17.833149185303718 8 23.214359410664795 40.732827723262616 20.43276327861358 15.62004958745901 9 25.896825396825395 16.264126984126985 20.592380952380953 37.24666666666667 10 13.784061761942251 30.880336236889555 35.28265230972395 20.052949691444244 11 33.17873604815055 23.445773494343072 25.646007136236083 17.7294833212703 12 22.715902814370878 25.841359125913417 32.96870734479059 18.474030714925117 13 28.491707007380366 23.514641695103563 26.13856043171177 21.855090865804303 14 19.250730889733262 24.29696502204573 28.394709024940717 28.05759506328029 15 21.270935710385636 33.95299226696126 23.478470661435118 21.297601361217986 16 19.97073257445417 29.711833308996717 30.013078285601825 20.304355830947287 17 19.97041514033762 30.51303701916667 29.203303854285046 20.31324398621066 18 19.69012081542476 28.99443220559573 31.810707687619434 19.504739291360078 19 22.211112063410436 27.959825665736588 28.423278004742453 21.405784266110526 20 22.31847530045139 29.001098322043262 29.391542285398664 19.288884092106684 21 21.242098779934153 27.92499912693701 28.1192953270494 22.71360676607944 22 21.525456548168886 30.298037945953837 28.334396734248173 19.8421087716291 23 20.28048389503122 29.98473146746152 29.089570100340605 20.645214537166655 24 21.826700568522696 28.229326375199587 30.207252076806114 19.7367209794716 25 21.02394473949025 29.21461381449255 29.376192395965933 20.385249050051268 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 156.0 1 156.0 2 171.5 3 187.0 4 187.0 5 187.0 6 292.0 7 397.0 8 397.0 9 397.0 10 674.5 11 952.0 12 952.0 13 952.0 14 1561.5 15 2171.0 16 2171.0 17 2171.0 18 3535.0 19 4899.0 20 4899.0 21 4899.0 22 7546.5 23 10194.0 24 10194.0 25 10194.0 26 14413.5 27 18633.0 28 18633.0 29 18633.0 30 24006.0 31 29379.0 32 29379.0 33 29379.0 34 32776.0 35 36173.0 36 36173.0 37 36173.0 38 37988.0 39 39803.0 40 39803.0 41 39803.0 42 40759.0 43 41715.0 44 41715.0 45 41715.0 46 41483.0 47 41251.0 48 41251.0 49 41251.0 50 37941.0 51 34631.0 52 34631.0 53 34631.0 54 29064.5 55 23498.0 56 23498.0 57 23498.0 58 19441.0 59 15384.0 60 15384.0 61 15384.0 62 12026.5 63 8669.0 64 8669.0 65 8669.0 66 6478.5 67 4288.0 68 4288.0 69 4288.0 70 2998.0 71 1708.0 72 1708.0 73 1708.0 74 1190.5 75 673.0 76 673.0 77 673.0 78 428.0 79 183.0 80 183.0 81 183.0 82 120.5 83 58.0 84 58.0 85 58.0 86 36.0 87 14.0 88 14.0 89 14.0 90 12.0 91 10.0 92 10.0 93 10.0 94 5.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1177676834049145 2 0.10443549283077323 3 0.01491935611868189 4 0.0 5 0.00476149663362188 6 0.3247340704130122 7 0.019998285861211895 8 0.00888812704942751 9 0.008570693940519384 10 0.003491764197989379 11 0.00476149663362188 12 3.174331089081253E-4 13 6.348662178162506E-4 14 0.0 15 0.00476149663362188 16 3.174331089081253E-4 17 3.174331089081253E-4 18 3.174331089081253E-4 19 0.0 20 3.174331089081253E-4 21 0.013967056791957515 22 0.0 23 0.0 24 0.0 25 0.0031743310890812535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 315027.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.9452362597375 #Duplication Level Percentage of deduplicated Percentage of total 1 94.69311480756079 81.3842212430626 2 4.116968072637292 7.076675873532162 3 0.5178952499326605 1.3353188883977491 4 0.19284452469985264 0.6629627294690247 5 0.10619338533781045 0.45634077960397307 6 0.07095565155499507 0.36589801421145973 7 0.04111919062662081 0.24737989872498767 8 0.03279227915962797 0.22546721438955938 9 0.02225229072208734 0.17212305450871448 >10 0.16962049861786976 2.8032173212698943 >50 0.018122024575398722 1.0791261665138194 >100 0.015163326685173145 2.3356168104270516 >500 0.002958697889789882 1.8556520058891028 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 881 0.2796585689480584 No Hit CTGTAGGACGTGGAATATGGCAAGA 873 0.2771191040767934 No Hit GTATCAACGCAGAGTACTTTTTTTT 834 0.2647392128293765 No Hit GTCCTAAAGTGTGTATTTCTCATTT 806 0.25585108577994903 No Hit CTTTAGGACGTGAAATATGGCGAGG 737 0.2339482012652884 No Hit GTCCTACAGTGTGCATTTCTCATTT 677 0.21490221473080087 No Hit CTGTAGGACCTGGAATATGGCGAGA 515 0.16347805108768457 No Hit GGTATCAACGCAGAGTACTTTTTTT 515 0.16347805108768457 No Hit TATCAACGCAGAGTACTTTTTTTTT 456 0.14474949766210515 No Hit CTGAAGGACCTGGAATATGGCGAGA 401 0.12729067667215826 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACC 25 0.005949202 19.039827 4 GTCTAGG 45 3.4333618E-5 16.940485 1 TTCAATA 40 0.005166625 14.291256 2 CAGTAGT 40 0.0051940274 14.279871 4 CAGTACA 50 0.0014714975 13.327879 4 GGCGAGG 200 0.0 13.287659 19 GCTACAC 60 3.999127E-4 12.695241 3 CTAGAGT 60 4.0045095E-4 12.693219 4 ACAGTGC 75 1.49112075E-5 12.648887 8 ACTGTTC 90 5.4513293E-7 12.648887 8 TCCTAGA 100 1.3754106E-7 12.385754 2 GTATAGA 55 0.0029899636 12.127847 1 AGAGGTG 55 0.0030114704 12.116254 5 GTCCTAA 160 3.6379788E-12 11.911279 1 ACTGTGC 65 8.087038E-4 11.675895 8 ACTGTCC 65 8.087038E-4 11.675895 8 AGTACAG 90 7.236842E-6 11.63545 5 GACGTGG 270 0.0 11.600337 7 AGGACGT 485 0.0 11.580926 5 GTCCTAG 75 2.0061123E-4 11.434828 1 >>END_MODULE