FastQCFastQC Report
Thu 26 May 2016
SRR1926927_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926927_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4025315
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19450.010.4496681
GTACCGT1909.640644E-1110.0202246
ACCGTGC3000.09.8276928
GTCCTAA3350.09.6844871
GTATCAA36100.09.54206851
GTCTAAG4450.09.4348541
GGGTAGG5050.09.258641
GTAGGAC5350.09.0892433
CACCGTA1054.4060653E-49.0658047
CTTATAC2900.08.8772233
GTATTAG2800.08.8604931
TAGGACC4200.08.8439034
GTACTAG2502.3646862E-118.7787031
GTATAAT5000.08.7787031
GTATAAG3700.08.7683871
GTCCTAG5500.08.6746071
CTTAGAC3750.08.6448813
GCCTAAG3450.08.5740321
GTATTAC3250.08.51446151
TAGGGTG3400.08.3995145
Produced by FastQC (version 0.11.5)