##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926927_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4025315 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.30141889516721 31.0 28.0 31.0 22.0 34.0 2 28.355089725897226 31.0 28.0 31.0 19.0 34.0 3 27.990887421232873 31.0 28.0 31.0 16.0 34.0 4 31.731305748742646 35.0 32.0 37.0 19.0 37.0 5 31.86118527369908 35.0 32.0 37.0 19.0 37.0 6 31.038113042084905 35.0 32.0 37.0 17.0 37.0 7 31.06918588979993 35.0 32.0 37.0 17.0 37.0 8 30.92940676692383 35.0 32.0 37.0 13.0 37.0 9 33.19856408753104 37.0 33.0 39.0 17.0 39.0 10 33.45735725030215 37.0 34.0 39.0 18.0 39.0 11 33.63701548822887 38.0 34.0 39.0 22.0 39.0 12 33.52352126479543 38.0 34.0 39.0 19.0 39.0 13 33.639831913775694 38.0 34.0 39.0 22.0 39.0 14 34.694913068915106 39.0 34.0 41.0 22.0 41.0 15 34.21180354829374 39.0 33.0 41.0 17.0 41.0 16 34.32230620460759 39.0 34.0 41.0 17.0 41.0 17 34.56214606807169 39.0 34.0 41.0 20.0 41.0 18 34.61161499162177 39.0 34.0 41.0 21.0 41.0 19 33.944710413967606 39.0 33.0 40.0 11.0 41.0 20 34.211180739892406 39.0 34.0 41.0 18.0 41.0 21 34.20479316525539 39.0 34.0 41.0 17.0 41.0 22 34.22722693751918 39.0 34.0 41.0 16.0 41.0 23 34.22674573294264 39.0 34.0 41.0 10.0 41.0 24 32.86713313119594 38.0 32.0 40.0 2.0 41.0 25 33.455444356528616 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.2227142737253942 1101 2 -0.4085189214378815 1101 3 -1.3863694355190077 1101 4 -0.6350184301463422 1101 5 -0.3440467324374019 1101 6 -2.5531985271313253 1101 7 -0.9116477581011253 1101 8 -0.27301897488356275 1101 9 -0.2759149798645453 1101 10 -9.167053650048896 1101 11 -3.9544054438454026 1101 12 -1.560538827494785 1101 13 -0.7866409891297295 1101 14 -0.5856463977358572 1101 15 -1.982209749134789 1101 16 -0.37018944447603275 1101 17 -0.2084807043886343 1101 18 -0.14991016142722913 1101 19 -0.9927364254213984 1101 20 -0.179746494616019 1101 21 -0.22805976664405136 1101 22 0.08634142891873609 1101 23 -0.08884569857756475 1101 24 -0.09163950889722372 1101 25 -0.005531583585579369 1102 1 -0.14292193788773488 1102 2 -0.33827242054845286 1102 3 -0.4873334801008191 1102 4 -0.41019390165124037 1102 5 -0.14921311910703494 1102 6 -1.2044950065476172 1102 7 -0.28208894794788364 1102 8 0.22422641198417992 1102 9 0.20936073167433733 1102 10 0.28893183905459807 1102 11 0.07555990081395692 1102 12 0.22837500684267553 1102 13 0.14132245284050526 1102 14 -0.09360588723654217 1102 15 0.48200664211926636 1102 16 0.3629033678131961 1102 17 0.1437870402331214 1102 18 -0.03954414643097692 1102 19 0.35886252872057867 1102 20 0.20595486842585586 1102 21 0.2033100319346417 1102 22 0.10374441046401728 1102 23 -0.3098382433140756 1102 24 -4.020275516040897 1102 25 -2.0113242162846348 1103 1 0.11625448498223179 1103 2 -0.17752662060944502 1103 3 0.12658014930010708 1103 4 -0.04852881268398335 1103 5 -0.2819062852770067 1103 6 0.2060224732609086 1103 7 0.16175558922825672 1103 8 0.35140851519402005 1103 9 0.31357889593633104 1103 10 0.31497526189539116 1103 11 0.092762142471976 1103 12 0.07766254167382414 1103 13 0.15820436522740522 1103 14 0.14948674073048807 1103 15 0.4819566956329169 1103 16 0.45197720383459483 1103 17 0.2102504813022108 1103 18 0.1200848239492629 1103 19 -1.5601355596911688 1103 20 -0.24774601382315353 1103 21 -0.009355202766883508 1103 22 0.07028872378909057 1103 23 0.23588854243919855 1103 24 1.7327307430686645 1103 25 0.9529478429107243 1104 1 -0.285807101119385 1104 2 -0.32764540724008384 1104 3 -1.4134767653828533 1104 4 -0.4636083651595726 1104 5 -0.2729824228353408 1104 6 -1.798652391508604 1104 7 -0.23914054542023067 1104 8 0.17860861768265224 1104 9 0.192018748390673 1104 10 0.4016532122667016 1104 11 0.312817659362878 1104 12 0.33108363321338885 1104 13 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10 -0.2181934160104717 1107 11 -0.5509981646454776 1107 12 -0.2737424856126296 1107 13 -0.46960549832610354 1107 14 -0.4018204620708943 1107 15 -1.7683345349303679 1107 16 -0.8359336538571469 1107 17 -0.7136848914941964 1107 18 -0.8566891632863616 1107 19 0.22718903058351003 1107 20 0.2174224386375343 1107 21 -0.17306629426635567 1107 22 -0.2673308215746104 1107 23 -0.586101767985781 1107 24 -4.581550602642803 1107 25 -2.4363357238417294 1108 1 0.33134338445247735 1108 2 -0.05013983059428284 1108 3 0.5103374877034206 1108 4 0.47811389870761545 1108 5 0.4957828657486516 1108 6 -3.205853220390946 1108 7 -1.439293157131619 1108 8 0.2653595733036376 1108 9 -0.028361790594196634 1108 10 0.42830789257464374 1108 11 0.27056206694182094 1108 12 0.34105203082528135 1108 13 0.3298118905457912 1108 14 0.17740697901512448 1108 15 -1.0243911298893238 1108 16 0.164348791528532 1108 17 0.06859134183380178 1108 18 0.09698267664757054 1108 19 0.2453789179020589 1108 20 -1.279206661572573 1108 21 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1110 8 0.9131634253257417 1110 9 0.6278290846765842 1110 10 0.670284493115588 1110 11 0.409371350842207 1110 12 0.5132206726281794 1110 13 0.3665577522700545 1110 14 0.1748736376450637 1110 15 0.7113024008455326 1110 16 0.5303058109221723 1110 17 0.05997663894849126 1110 18 0.30235400003059 1110 19 -1.8928172825673926 1110 20 -0.10129720471948644 1110 21 -0.14544756014118576 1110 22 0.1099281340072551 1110 23 0.11735433184606592 1110 24 -1.0097596534073219 1110 25 -0.6495955975727057 1111 1 0.45361483905604416 1111 2 0.2326087497208924 1111 3 0.9142223112571735 1111 4 0.8253311630257265 1111 5 0.6736451059617536 1111 6 0.8978376405107653 1111 7 0.7972362107653339 1111 8 1.1894218500334297 1111 9 0.5178770138844868 1111 10 0.4243055216720961 1111 11 0.3365587561719039 1111 12 0.3941258822134728 1111 13 0.2970232800034367 1111 14 0.43370755627017843 1111 15 -0.5641126097595048 1111 16 -0.19741217445355375 1111 17 0.12699049826461106 1111 18 0.343462780603069 1111 19 0.9539901762495901 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-1.237389543195846 2215 1 0.06085130623131363 2215 2 -0.34817832941216764 2215 3 0.06690373171140607 2215 4 0.37277239868109646 2215 5 0.35205806245882343 2215 6 1.0968667896000852 2215 7 0.529801682937876 2215 8 -0.8475176041921415 2215 9 -0.47692498935470695 2215 10 -0.701775450911228 2215 11 -0.7980410526536872 2215 12 -0.957784558859089 2215 13 -0.6188965607077819 2215 14 -0.6049756175915064 2215 15 -0.6217455728201813 2215 16 -0.3945089983022996 2215 17 -0.8449023570065961 2215 18 -1.6406486054613865 2215 19 -0.35370554392869735 2215 20 -4.336073643073114 2215 21 -2.7645702292300918 2215 22 -1.8071488971365923 2215 23 -1.5644863758621952 2215 24 -1.461503674443204 2215 25 -1.2892897046309173 2216 1 -0.635186917460075 2216 2 -1.0504781960946232 2216 3 -0.21556998189344156 2216 4 -0.5052562919753889 2216 5 -0.7085804081621596 2216 6 -1.7938920269093437 2216 7 -10.047912935769293 2216 8 -11.351945336752621 2216 9 -6.3582463950415615 2216 10 -3.979043025140694 2216 11 -2.865256111124175 2216 12 -2.42012083961502 2216 13 -2.2236402731744604 2216 14 -2.274999037762207 2216 15 -3.291863052248509 2216 16 -2.3623293259275293 2216 17 -2.6869472071528904 2216 18 -2.4190275362932496 2216 19 -1.7696212338028232 2216 20 -2.774501772420752 2216 21 -5.384869220024598 2216 22 -3.909128023447316 2216 23 -3.8104959226977364 2216 24 -3.8216531217644665 2216 25 -3.3706674214403805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 213308.0 3 41.0 4 1642.0 5 9836.0 6 12703.0 7 10268.0 8 7974.0 9 7126.0 10 6808.0 11 6695.0 12 6757.0 13 6775.0 14 7068.0 15 7286.0 16 7936.0 17 8731.0 18 10016.0 19 12278.0 20 15955.0 21 20948.0 22 26510.0 23 32078.0 24 37667.0 25 43382.0 26 49744.0 27 54422.0 28 61336.0 29 73081.0 30 87115.0 31 107233.0 32 137781.0 33 180846.0 34 243266.0 35 337051.0 36 483785.0 37 750697.0 38 939170.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.390953305503835 16.737261232926713 11.848284591876928 26.023500869692523 2 16.199355171081653 19.000885825082257 38.87436283902834 25.925396164807747 3 19.20567840953586 22.76709774325362 29.259839526669847 28.767384320540675 4 13.105879785018038 16.028705695520447 35.56497650576995 35.30043801369157 5 14.518593431824083 36.16962010533234 34.88396421334334 14.427822249500236 6 34.04919240978004 34.2470831981179 17.533586022608848 14.170138369493213 7 29.88047431452609 30.991019685513287 20.7044187294374 18.42408727052322 8 27.16167716006969 34.44281039276804 19.587311817184258 18.80820062997801 9 26.99081918219186 15.23408488871032 18.50798603682813 39.267109892269694 10 15.655581141402699 27.752498430637146 32.91096804464098 23.680952383319177 11 35.801554105728286 21.889987164241557 22.646491882364224 19.661966847665933 12 24.21949424469405 23.694179396188574 29.59503895261035 22.491287406507027 13 29.223386003729455 20.681837099024403 24.825335458535573 25.269441438710565 14 22.839888387255474 19.95400819454211 25.996029448669816 31.210073969532605 15 24.873178174694967 27.615615514141155 22.53536314510673 24.975843166057146 16 24.647569381031573 26.680268183953938 24.601949385161227 24.070213049853262 17 23.77981480864902 26.73119325825164 25.51867343637644 23.9703184967229 18 23.78936990510947 26.649453145207037 25.959868741929608 23.60130820775389 19 25.100021543675144 25.954487930199488 25.631382500124865 23.314108026000508 20 25.062173092772515 25.72913775629241 25.697756153266454 23.51093299766862 21 24.98925392685117 25.40359269177579 25.527427208709362 24.07972617266368 22 24.967363686554236 25.32953110550015 25.427700937570346 24.275404270375272 23 24.689169643964167 25.422604800499325 25.368984871378014 24.51924068415849 24 24.828863318497472 25.693895232621905 25.19517569850426 24.282065750376365 25 24.591909702086255 25.369876478425095 25.484095547468872 24.55411827201978 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 649.0 1 649.0 2 816.0 3 983.0 4 983.0 5 983.0 6 1328.5 7 1674.0 8 1674.0 9 1674.0 10 2338.5 11 3003.0 12 3003.0 13 3003.0 14 5385.5 15 7768.0 16 7768.0 17 7768.0 18 15081.0 19 22394.0 20 22394.0 21 22394.0 22 37860.5 23 53327.0 24 53327.0 25 53327.0 26 80215.0 27 107103.0 28 107103.0 29 107103.0 30 144216.0 31 181329.0 32 181329.0 33 181329.0 34 227075.0 35 272821.0 36 272821.0 37 272821.0 38 324305.5 39 375790.0 40 375790.0 41 375790.0 42 422288.5 43 468787.0 44 468787.0 45 468787.0 46 507515.0 47 546243.0 48 546243.0 49 546243.0 50 558581.5 51 570920.0 52 570920.0 53 570920.0 54 538972.0 55 507024.0 56 507024.0 57 507024.0 58 447585.0 59 388146.0 60 388146.0 61 388146.0 62 324005.0 63 259864.0 64 259864.0 65 259864.0 66 204020.0 67 148176.0 68 148176.0 69 148176.0 70 108555.5 71 68935.0 72 68935.0 73 68935.0 74 48164.5 75 27394.0 76 27394.0 77 27394.0 78 18346.0 79 9298.0 80 9298.0 81 9298.0 82 6026.0 83 2754.0 84 2754.0 85 2754.0 86 1755.0 87 756.0 88 756.0 89 756.0 90 450.5 91 145.0 92 145.0 93 145.0 94 81.5 95 18.0 96 18.0 97 18.0 98 16.0 99 14.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22241737603144102 2 0.13251136867549496 3 0.13556703015788826 4 0.07050379908156255 5 0.022606926414454522 6 0.021687743692108567 7 0.3197762162712732 8 0.3178384797214628 9 0.018085541131563618 10 0.03450661625239267 11 0.015427364069644239 12 0.01691793064642146 13 0.014905665767772212 14 0.017017301751539943 15 0.014259753584502084 16 0.01897988107762995 17 0.017911641697606274 18 0.01421006803194284 19 0.023153467492606168 20 0.016495603449667915 21 0.014632395228696388 22 0.01632170401571057 23 0.017191201185497284 24 0.015104407978009174 25 0.01473176633381487 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4025315.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.03820931887418 #Duplication Level Percentage of deduplicated Percentage of total 1 87.78717595104885 67.62966836429739 2 7.737718545700408 11.921999619484055 3 2.0865549165555075 4.8223336325078785 4 0.856900197985353 2.640562272711214 5 0.4481422979147281 1.7262040075698049 6 0.2713392005653435 1.254209167774135 7 0.17843869289951378 0.9622618161925336 8 0.11538034174031148 0.7110955934618681 9 0.0903154011905834 0.6261963103474436 >10 0.4042625231053684 5.2814045465395925 >50 0.01661560698999511 0.8544449890202896 >100 0.006352413175830465 0.8424404077715562 >500 4.180338046190205E-4 0.22414384409380214 >1k 3.8587732368940256E-4 0.5030354282284681 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.452832883886106E-5 2 0.0 0.0 0.0 0.0 7.452832883886106E-5 3 0.0 0.0 0.0 0.0 7.452832883886106E-5 4 0.0 0.0 0.0 0.0 7.452832883886106E-5 5 0.0 2.484277627962035E-5 0.0 0.0 7.452832883886106E-5 6 2.484277627962035E-5 2.484277627962035E-5 0.0 0.0 9.93711051184814E-5 7 2.484277627962035E-5 2.484277627962035E-5 0.0 0.0 9.93711051184814E-5 8 2.484277627962035E-5 2.484277627962035E-5 0.0 0.0 9.93711051184814E-5 9 2.484277627962035E-5 2.484277627962035E-5 0.0 2.484277627962035E-5 9.93711051184814E-5 10 2.484277627962035E-5 2.484277627962035E-5 0.0 2.484277627962035E-5 9.93711051184814E-5 11 2.484277627962035E-5 2.484277627962035E-5 0.0 4.96855525592407E-5 9.93711051184814E-5 12 2.484277627962035E-5 2.484277627962035E-5 0.0 4.96855525592407E-5 9.93711051184814E-5 13 2.484277627962035E-5 4.96855525592407E-5 0.0 4.96855525592407E-5 1.7389943395734246E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1945 0.0 10.449668 1 GTACCGT 190 9.640644E-11 10.020224 6 ACCGTGC 300 0.0 9.827692 8 GTCCTAA 335 0.0 9.684487 1 GTATCAA 3610 0.0 9.5420685 1 GTCTAAG 445 0.0 9.434854 1 GGGTAGG 505 0.0 9.25864 1 GTAGGAC 535 0.0 9.089243 3 CACCGTA 105 4.4060653E-4 9.065804 7 CTTATAC 290 0.0 8.877223 3 GTATTAG 280 0.0 8.860493 1 TAGGACC 420 0.0 8.843903 4 GTACTAG 250 2.3646862E-11 8.778703 1 GTATAAT 500 0.0 8.778703 1 GTATAAG 370 0.0 8.768387 1 GTCCTAG 550 0.0 8.674607 1 CTTAGAC 375 0.0 8.644881 3 GCCTAAG 345 0.0 8.574032 1 GTATTAC 325 0.0 8.5144615 1 TAGGGTG 340 0.0 8.399514 5 >>END_MODULE