FastQCFastQC Report
Thu 26 May 2016
SRR1926927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926927_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4025315
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51290.1274185995381728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTG803.6908826E-410.7131275
CCGTGCG1106.141916E-610.3508019
GTACTAA1855.638867E-1110.3053051
GGTATCA20600.09.9488711
GTATCAA39300.09.8477271
GTATAGC3950.09.4117441
AACCGCT1054.545204E-49.0376497
GTTCTAG4750.09.0307021
CGTAGTA950.00176968869.0303632
ACTAGAC1901.015178E-89.0224813
GTCCTAG4650.09.0199121
GTGTATA4550.09.0086481
TAGACTG4900.08.9397535
ACCGGTC850.0075165598.9298828
ACCGTGC2451.6370905E-118.9071028
CTAGACA4100.08.8259914
GTACTAG1752.4951987E-78.71534251
GTCCTAA5200.08.6158291
TCTATAC3650.08.6104953
CGTACAC1554.071808E-68.60207753
Produced by FastQC (version 0.11.5)