FastQCFastQC Report
Thu 26 May 2016
SRR1926926_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926926_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4704754
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56590.12028259075819905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21850.011.8276311
GTCCTAC6850.010.1627731
TATGCCG1553.9784936E-89.8209375
GTATCAA42950.09.74723151
CTAATAC2251.8189894E-129.7402033
GTCTTAA4950.09.2473221
CTAGTAC2700.09.1755533
GTATAGA5500.09.0161391
GTAGGAC6900.08.9760853
GTCTTAG5600.08.8551361
TAATACC3550.08.848374
GTATTAA6150.08.8385231
ATAATAC4350.08.7618023
TAGGACC4900.08.7416644
TATACCG1554.128744E-68.593325
CTTATAC3900.08.5511813
GTACTAG2900.08.54978751
TCTATAC5100.08.4074653
GTAATAC3300.08.3734953
GTATTGG5250.08.3556171
Produced by FastQC (version 0.11.5)