FastQCFastQC Report
Thu 26 May 2016
SRR1926926_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926926_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4704754
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT98110.20853375118018921No Hit
GGTATCAACGCAGAGTACTTTTTTT59570.12661660949754228No Hit
TATCAACGCAGAGTACTTTTTTTTT53840.11443743923699305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG907.283901E-611.6373775
GGTATCA27150.011.4795041
GTATCAA55800.010.0777371
TAGGACC5450.09.9582574
TAGACCG1252.4998808E-69.902355
GTACTAA3000.09.5311481
GTCCTAC6700.09.1043811
GTCCTAA4950.09.0497771
GTAGGAC6750.08.8873883
TAGTACC2800.08.8413844
CGTGCCG1304.4369808E-58.75833610
CTAACAC4900.08.7448793
GTCCTAG4600.08.7023521
GTATAAG4200.08.623421
GTATTAT4300.08.4228741
TACGGGA1701.5415153E-68.4091192
ACCGTGC3500.08.4040998
TACACCG2303.5470293E-98.2795575
GTACCGT2501.9863364E-97.99805266
TCTACAC7050.07.96893453
Produced by FastQC (version 0.11.5)