FastQCFastQC Report
Thu 26 May 2016
SRR1926925_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926925_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969325
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12860.1326696412451964No Hit
GTCCTAAAGTGTGTATTTCTCATTT11240.11595698037293993No Hit
CTGTAGGACGTGGAATATGGCAAGA9800.10110128181982307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGC250.006098763618.96299210
TAGGACC2600.013.5530594
ATAGGAC852.5752888E-713.4620753
AGGACCT4850.011.5820155
TCCAGTA855.1187504E-511.2183962
TTAGGAC4350.011.1797123
GTTTAAG600.00568064711.1329991
GGTCTAT951.2928993E-511.04929351
CTGGACA2000.010.9523364
CTACACT1408.00901E-910.8843114
GGCGAGG3600.010.79725619
AGGACGT6800.010.7809175
TAGGACA3200.010.7142434
GGACCTG4800.010.710916
GTAGGAC8550.010.706683
GGACGTG6600.010.6748476
TGGCGAG7650.010.65783618
GTATAGG909.084204E-510.6028581
TGTAGGA9100.010.58350852
TATGGCG7750.010.52031516
Produced by FastQC (version 0.11.5)