##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926925_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 969325 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.279645113867897 31.0 28.0 31.0 16.0 34.0 2 28.447377298635647 31.0 28.0 33.0 16.0 34.0 3 28.099775617053105 31.0 28.0 33.0 16.0 34.0 4 31.85146777396642 35.0 32.0 37.0 19.0 37.0 5 31.894670002321202 35.0 32.0 37.0 19.0 37.0 6 31.42711887137957 35.0 32.0 37.0 17.0 37.0 7 31.351402264462383 35.0 32.0 37.0 17.0 37.0 8 30.974141799705983 35.0 32.0 37.0 11.0 37.0 9 33.17270987542878 37.0 33.0 39.0 17.0 39.0 10 33.35665643617982 37.0 34.0 39.0 17.0 39.0 11 33.530270033270575 38.0 34.0 39.0 19.0 39.0 12 33.39383385345472 38.0 34.0 39.0 17.0 39.0 13 33.50590668764346 38.0 34.0 39.0 18.0 39.0 14 34.53939287648622 39.0 34.0 41.0 18.0 41.0 15 34.09534676192196 39.0 33.0 41.0 16.0 41.0 16 34.194343486446755 39.0 34.0 41.0 17.0 41.0 17 34.4294215046553 39.0 34.0 41.0 17.0 41.0 18 34.48931369767622 39.0 34.0 41.0 17.0 41.0 19 33.85976323730431 39.0 33.0 41.0 10.0 41.0 20 34.12588863384314 39.0 34.0 41.0 11.0 41.0 21 34.103186237845925 39.0 34.0 41.0 10.0 41.0 22 34.1292817166585 39.0 34.0 41.0 10.0 41.0 23 34.13649601526836 39.0 34.0 41.0 2.0 41.0 24 32.85127279292291 39.0 32.0 41.0 2.0 41.0 25 33.425002965981484 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.32989382340441153 1101 2 -0.49503907949429404 1101 3 -1.3017790932580766 1101 4 -0.7007103541949391 1101 5 -0.6006750773550138 1101 6 -2.789429219224509 1101 7 -1.131234574423548 1101 8 -0.8370613956744712 1101 9 -0.8488384652410375 1101 10 -8.93581838158757 1101 11 -4.073125943159319 1101 12 -1.9724789581813411 1101 13 -1.3943671095817294 1101 14 -1.1822248233263437 1101 15 -2.6372037439559577 1101 16 -0.8975179199121186 1101 17 -0.7194878870835879 1101 18 -0.47755123026886537 1101 19 -1.3515981454316588 1101 20 -0.5640709775928983 1101 21 -0.6135477560609033 1101 22 -0.4026120324049245 1101 23 -0.668774502797234 1101 24 -0.9039375354597752 1101 25 -0.7316577227940613 1102 1 0.20343047633996392 1102 2 0.09175582457245213 1102 3 -0.05862488049826098 1102 4 -0.06579279640922664 1102 5 0.2335898860515293 1102 6 -0.6028107668662237 1102 7 0.21459049803885577 1102 8 0.7802807213405103 1102 9 0.6265895958227219 1102 10 0.6664392903279719 1102 11 0.28566298884792474 1102 12 0.30746850193775543 1102 13 0.17505511492636572 1102 14 0.06525314864612852 1102 15 0.6927658962615837 1102 16 0.5835231649493835 1102 17 0.39127710129720583 1102 18 0.2965040384812312 1102 19 0.6975852956041422 1102 20 0.47537992526321204 1102 21 0.18301647992743142 1102 22 0.2971718043614189 1102 23 -0.15022407732013932 1102 24 -3.461492294586872 1102 25 -1.6857189556910335 1103 1 0.25895052053983747 1103 2 0.2540870252080296 1103 3 0.4106484429008539 1103 4 0.37589492501863475 1103 5 0.134011115703359 1103 6 0.9684469131645841 1103 7 0.7803952805782934 1103 8 0.8443730611714102 1103 9 0.7692662692981642 1103 10 0.6622547195527204 1103 11 0.5746731092482236 1103 12 0.22656982490939015 1103 13 0.4165291969018057 1103 14 0.3922692134457222 1103 15 0.8369886579875541 1103 16 0.7533070453032238 1103 17 0.352045174952913 1103 18 0.18726914800296157 1103 19 -1.3372639469285588 1103 20 -0.09823545113139431 1103 21 0.2062158693561571 1103 22 0.40813526888960894 1103 23 0.3159046427184151 1103 24 1.8067704002746865 1103 25 0.9060688808742796 1104 1 -0.5403066761252262 1104 2 -0.4621837221620524 1104 3 -1.5423959191895023 1104 4 -0.7384080805463249 1104 5 -0.4890909571140014 1104 6 -1.5503868694340923 1104 7 -0.504264496904014 1104 8 -0.026336522123191486 1104 9 0.1141080699698378 1104 10 0.054322929752324 1104 11 0.029904861463904808 1104 12 -0.014782659117102526 1104 13 -0.1860649568560575 1104 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0.3066130371274767 2215 2 0.09927597636218977 2215 3 0.40558931906621254 2215 4 0.351426785961408 2215 5 0.5021510181638256 2215 6 0.9786323566144759 2215 7 0.3644762197978082 2215 8 -0.5409364197728799 2215 9 -0.19239325517547456 2215 10 -0.3475638941011212 2215 11 -0.23720490716614506 2215 12 -0.429426658934041 2215 13 -0.15735319780367263 2215 14 -0.07284603365724962 2215 15 -0.2192761863633521 2215 16 -0.06157449572000928 2215 17 -0.38615372455770114 2215 18 -1.0615187922561873 2215 19 0.12333101042958816 2215 20 -3.5806631205788655 2215 21 -2.1691925880146705 2215 22 -1.2606799722967565 2215 23 -1.0363149311166495 2215 24 -1.1081838305237994 2215 25 -0.9109747583477699 2216 1 -0.8413278583718586 2216 2 -1.387206580557205 2216 3 -0.6661483404936099 2216 4 -0.8722418769344173 2216 5 -1.1077414809505512 2216 6 -2.195157775734341 2216 7 -9.828850095673555 2216 8 -11.197110207554747 2216 9 -6.228125253436119 2216 10 -3.7400081079746634 2216 11 -2.819924513359993 2216 12 -2.5504481775792236 2216 13 -2.242904293043445 2216 14 -2.4242595955915647 2216 15 -3.548476032574797 2216 16 -2.388933244458727 2216 17 -2.529565916497905 2216 18 -2.2116056760736384 2216 19 -1.6871558243635576 2216 20 -2.6221104790589784 2216 21 -4.919335547571304 2216 22 -3.6351692045795936 2216 23 -3.424406727437475 2216 24 -3.407268654313345 2216 25 -2.727440878695706 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 55312.0 3 12.0 4 457.0 5 2593.0 6 3291.0 7 2737.0 8 2084.0 9 1920.0 10 1796.0 11 1841.0 12 1732.0 13 1751.0 14 1776.0 15 1813.0 16 1932.0 17 2048.0 18 2419.0 19 2906.0 20 3863.0 21 5064.0 22 6170.0 23 7437.0 24 8771.0 25 9999.0 26 11316.0 27 12436.0 28 13880.0 29 16341.0 30 19676.0 31 23505.0 32 30622.0 33 40370.0 34 54058.0 35 74416.0 36 108940.0 37 174651.0 38 259390.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66159971045965 18.544025645002844 13.056822294607311 25.737552349930198 2 14.504279729527292 19.927681094288356 41.48336406886826 24.084675107316087 3 18.192910623939067 24.635215037932674 29.941347186018376 27.23052715210988 4 12.079584896544176 17.397633348681534 37.83648177934518 32.68629997542911 5 13.24179662629517 37.40608389580665 36.0580053514508 13.294114126447374 6 32.251999768860564 36.35276417999158 18.647896218393743 12.74733983275411 7 27.922118244648814 31.06980296698315 23.096682584135934 17.9113962042321 8 25.209682226895623 35.7707943499644 21.661126198479856 17.35839722466012 9 26.56666611634376 15.381559505039583 20.150588983085825 37.90118539553083 10 15.125849220363333 28.726997648034992 34.33796674224466 21.809186389357023 11 34.048686970617155 22.406778576742393 24.521678836861607 19.022855615778848 12 23.60887406736342 24.56072830004509 31.388592813784292 20.441804818807192 13 28.813547080375944 21.862021929810986 25.664709347976594 23.659721641836477 14 21.324777461696172 21.768254744577515 27.42584034198723 29.481127451739088 15 23.58370254503282 30.296786362107426 23.058930108070516 23.060580984789233 16 22.51164441656159 27.579261484995882 27.656650163960816 22.252443934481715 17 22.165161735010273 28.171164952272466 27.453833113036513 22.209840199680748 18 21.89658712544483 27.507498583858776 28.913734557869873 21.682179732826516 19 24.132020761745554 26.75406824959498 26.892651869279426 22.221259119380036 20 24.076733858319713 27.0556807488485 27.270710547603716 21.596874845228072 21 23.61702391098436 26.525261613449747 26.662388126845638 23.195326348720254 22 23.638025801645338 27.604231692708037 26.531140788494817 22.226601717151812 23 22.79454327644861 27.33489964948775 26.99295157730513 22.87760549675851 24 23.783134021895023 26.71760372277309 27.235675887615173 22.263586367716705 25 23.10126582278481 26.856636386523753 27.21756303318709 22.824534757504345 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 171.0 1 171.0 2 234.0 3 297.0 4 297.0 5 297.0 6 426.0 7 555.0 8 555.0 9 555.0 10 833.5 11 1112.0 12 1112.0 13 1112.0 14 1913.0 15 2714.0 16 2714.0 17 2714.0 18 5150.5 19 7587.0 20 7587.0 21 7587.0 22 13294.5 23 19002.0 24 19002.0 25 19002.0 26 29496.5 27 39991.0 28 39991.0 29 39991.0 30 54123.5 31 68256.0 32 68256.0 33 68256.0 34 80379.0 35 92502.0 36 92502.0 37 92502.0 38 101625.0 39 110748.0 40 110748.0 41 110748.0 42 117329.5 43 123911.0 44 123911.0 45 123911.0 46 128200.5 47 132490.0 48 132490.0 49 132490.0 50 127674.5 51 122859.0 52 122859.0 53 122859.0 54 109519.5 55 96180.0 56 96180.0 57 96180.0 58 82095.0 59 68010.0 60 68010.0 61 68010.0 62 55486.0 63 42962.0 64 42962.0 65 42962.0 66 33145.0 67 23328.0 68 23328.0 69 23328.0 70 16948.5 71 10569.0 72 10569.0 73 10569.0 74 7303.5 75 4038.0 76 4038.0 77 4038.0 78 2735.0 79 1432.0 80 1432.0 81 1432.0 82 932.5 83 433.0 84 433.0 85 433.0 86 283.5 87 134.0 88 134.0 89 134.0 90 81.0 91 28.0 92 28.0 93 28.0 94 19.0 95 10.0 96 10.0 97 10.0 98 8.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2346994042245893 2 0.1433987568668919 3 0.14711268150517112 4 0.07211203672658809 5 0.024965826735099166 6 0.02197405410981869 7 0.3188816960255848 8 0.31991334175844016 9 0.020323420937250147 10 0.03590127150336574 11 0.0158873442859722 12 0.016918990018827535 13 0.014649369406545792 14 0.0171253191653986 15 0.014752533979831325 16 0.019704433497536946 17 0.018363294044825007 18 0.014649369406545792 19 0.02424367472210043 20 0.016196838005828797 21 0.015165192272973459 22 0.01671266087225647 23 0.017744306605111806 24 0.01599050885925773 25 0.015784179712686666 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 969325.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.76535108525304 #Duplication Level Percentage of deduplicated Percentage of total 1 91.29107077954687 76.47028594797428 2 6.414953748663132 10.747017059048549 3 1.2125931707169983 3.0471987800606866 4 0.42370952129392825 1.419687072374016 5 0.1912856032386962 0.8011552856421904 6 0.11880328177439957 0.5970959164747696 7 0.06438655233577263 0.377535351310251 8 0.048068314906907404 0.3221167419402881 9 0.03180427230927047 0.239768643239733 >10 0.17956049356772824 2.6533399107653235 >50 0.012686931516190963 0.7342165221548065 >100 0.009969597818371112 1.7330007952481783 >500 8.615695758321435E-4 0.6091120823533553 >1k 2.46162735952041E-4 0.24846989141360634 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1286 0.1326696412451964 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1124 0.11595698037293993 No Hit CTGTAGGACGTGGAATATGGCAAGA 980 0.10110128181982307 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 1.0316457328553375E-4 13 0.0 0.0 0.0 0.0 1.0316457328553375E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGCGC 25 0.0060987636 18.962992 10 TAGGACC 260 0.0 13.553059 4 ATAGGAC 85 2.5752888E-7 13.462075 3 AGGACCT 485 0.0 11.582015 5 TCCAGTA 85 5.1187504E-5 11.218396 2 TTAGGAC 435 0.0 11.179712 3 GTTTAAG 60 0.005680647 11.132999 1 GGTCTAT 95 1.2928993E-5 11.0492935 1 CTGGACA 200 0.0 10.952336 4 CTACACT 140 8.00901E-9 10.884311 4 GGCGAGG 360 0.0 10.797256 19 AGGACGT 680 0.0 10.780917 5 TAGGACA 320 0.0 10.714243 4 GGACCTG 480 0.0 10.71091 6 GTAGGAC 855 0.0 10.70668 3 GGACGTG 660 0.0 10.674847 6 TGGCGAG 765 0.0 10.657836 18 GTATAGG 90 9.084204E-5 10.602858 1 TGTAGGA 910 0.0 10.5835085 2 TATGGCG 775 0.0 10.520315 16 >>END_MODULE