FastQCFastQC Report
Thu 26 May 2016
SRR1926925_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926925_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969325
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13170.13586774301704796No Hit
GTATCAACGCAGAGTACTTTTTTTT11980.12359115879606944No Hit
CTTTAGGACGTGAAATATGGCGAGG11680.12049622159750342No Hit
CTGTAGGACGTGGAATATGGCAAGA11480.11843293013179275No Hit
GTCCTAAAGTGTGTATTTCTCATTT11350.1170917906790808No Hit
GTACATGGGAAGCAGTGGTATCAAC10320.10646583963067084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3100.012.2890054
GTGTTAC657.7658845E-411.7370191
TAAGACC1302.5065674E-911.7218224
GTATTAG907.1422346E-611.6555111
TTAGGAC4900.011.2738673
CCTATCC700.001458864510.8851123
TAGTGGG803.80996E-410.6746717
TGGCGAG8000.010.6735718
GTCCAGA1355.3649273E-810.595921
GGCGAGG3900.010.46070719
GTATAGG1105.7675898E-610.4032671
GTCCTAA3500.010.3537261
CTAAGAC1201.4715897E-610.3181793
TGTAGGA7600.010.2881282
AGGACGT6600.010.2454945
TAGGACT1409.3152266E-810.2042654
GTCTAGG750.002563540810.1720821
GTCTAGA750.002563540810.1720821
CTGTAGG7900.010.1398921
TTTAGGA5750.010.115752
Produced by FastQC (version 0.11.5)