FastQCFastQC Report
Thu 26 May 2016
SRR1926924_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926924_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659706
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11420.1731074145149506No Hit
GTCCTAAAGTGTGTATTTCTCATTT10090.15294691877897124No Hit
GTATCAACGCAGAGTACTTTTTTTT9180.13915289538066958No Hit
CTGTAGGACGTGGAATATGGCAAGA8970.1359696592118307No Hit
GTCCTACAGTGTGCATTTCTCATTT8870.1344538324647646No Hit
CTTTAGGACGTGAAATATGGCGAGG7830.11868923429527699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCG551.9830959E-413.7969999
TAGGACC2350.013.7790044
CCTAGAC500.001459952613.3454073
GTAGGAC5450.012.9431213
GGACCTG3800.012.7766
TGTAGGA6100.012.6578222
GGACGTG4400.012.54826456
AGGACCT3700.012.3494575
AGGACGT4350.012.2548245
GACGTGG2200.012.1155657
CGTGGAA2350.012.1090699
CCTACAC959.94909E-712.0409693
TAGGACG4600.012.0081714
CTGTAGG6300.011.9597511
GGCGAGG2300.011.95536419
TAGGACA2100.011.7912754
GTATCAG657.751906E-411.738491
GTATTGG907.122524E-611.6569731
TGGCGAG5050.011.45331418
CTACACT1001.8669471E-611.4284674
Produced by FastQC (version 0.11.5)