##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926924_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 659706 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.070598114917857 31.0 28.0 31.0 16.0 34.0 2 28.232552076227897 31.0 28.0 33.0 16.0 34.0 3 27.864641218967236 31.0 28.0 31.0 16.0 34.0 4 31.57063904224003 35.0 32.0 37.0 18.0 37.0 5 31.643667027433434 35.0 32.0 37.0 18.0 37.0 6 31.159821193076915 35.0 32.0 37.0 13.0 37.0 7 31.08782700172501 35.0 32.0 37.0 11.0 37.0 8 30.682364568459285 35.0 31.0 37.0 10.0 37.0 9 32.87028464194656 37.0 33.0 39.0 17.0 39.0 10 33.05896869211437 37.0 33.0 39.0 17.0 39.0 11 33.231363668058194 38.0 33.0 39.0 17.0 39.0 12 33.09580782954831 38.0 33.0 39.0 17.0 39.0 13 33.20596447508436 38.0 33.0 39.0 17.0 39.0 14 34.21331926646112 39.0 34.0 41.0 17.0 41.0 15 33.78538318584339 39.0 33.0 41.0 10.0 41.0 16 33.88616292712208 39.0 33.0 41.0 11.0 41.0 17 34.11321558391162 39.0 34.0 41.0 16.0 41.0 18 34.169142011744626 39.0 34.0 41.0 12.0 41.0 19 33.542456791358575 39.0 33.0 41.0 10.0 41.0 20 33.807286882338495 39.0 34.0 41.0 10.0 41.0 21 33.79295625627174 39.0 34.0 41.0 8.0 41.0 22 33.80596204976156 39.0 34.0 41.0 2.0 41.0 23 33.80010944269114 39.0 34.0 41.0 2.0 41.0 24 32.51186589177603 39.0 32.0 40.0 2.0 41.0 25 33.08157421639336 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.250787972349638 1101 2 -0.38981853653094944 1101 3 -1.0790864662771575 1101 4 -0.6011126811708927 1101 5 -0.5896107791401448 1101 6 -2.1232135352161308 1101 7 -0.862856673818591 1101 8 -0.3467902121234374 1101 9 -0.6283339550348188 1101 10 -9.161463197163577 1101 11 -4.284166175071189 1101 12 -1.9631202356731734 1101 13 -1.2678720431977837 1101 14 -0.9702980003633002 1101 15 -2.5286280701266257 1101 16 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-0.6781489149622999 2215 1 1.0127379255429112 2215 2 0.4889900862057708 2215 3 0.9191956649190445 2215 4 1.1993195687549019 2215 5 1.2024389759800727 2215 6 1.588071341601914 2215 7 1.331051013922579 2215 8 0.2025187445402601 2215 9 0.7382585056360043 2215 10 0.5539364021240445 2215 11 0.5649730353770153 2215 12 0.06804813773139529 2215 13 0.5694220351637469 2215 14 0.7285416033766836 2215 15 0.7278658820847141 2215 16 0.9348497918817955 2215 17 0.46760852549448373 2215 18 -0.2833925698045334 2215 19 0.7681923223586935 2215 20 -2.9269842572424842 2215 21 -1.1398880360278838 2215 22 -0.474877856275711 2215 23 -0.33412892307413955 2215 24 -0.16697363926764552 2215 25 0.14145611019611692 2216 1 -0.8936385597211505 2216 2 -1.3447285952680978 2216 3 -0.5839139258953665 2216 4 -0.9123369836672204 2216 5 -0.8685893033692196 2216 6 -2.3468277055538707 2216 7 -9.628972679692307 2216 8 -10.473630336939884 2216 9 -5.807996216415145 2216 10 -3.720081038573266 2216 11 -2.9682337227951265 2216 12 -2.4294840389037162 2216 13 -2.4120075057528503 2216 14 -2.335069476134226 2216 15 -3.583359164106067 2216 16 -2.6342488700566093 2216 17 -2.7631053034956743 2216 18 -2.4836012619091505 2216 19 -1.9988382478964013 2216 20 -2.7861955423072686 2216 21 -5.1412861162174295 2216 22 -3.6648818182874727 2216 23 -3.5337476284769913 2216 24 -3.783785906597526 2216 25 -3.294302418916825 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40576.0 3 5.0 4 335.0 5 1892.0 6 2472.0 7 2087.0 8 1624.0 9 1455.0 10 1352.0 11 1317.0 12 1283.0 13 1205.0 14 1243.0 15 1272.0 16 1465.0 17 1551.0 18 1725.0 19 2087.0 20 2672.0 21 3496.0 22 4616.0 23 5249.0 24 6128.0 25 6945.0 26 7907.0 27 8708.0 28 9536.0 29 11239.0 30 13642.0 31 16504.0 32 21256.0 33 27727.0 34 37536.0 35 51530.0 36 74349.0 37 118039.0 38 167681.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.606490779762574 18.93969548452963 12.920638200253409 25.53317553545439 2 13.842517963990057 19.978962323433784 43.19265715265825 22.985862559917912 3 18.39518391237295 24.852609017717274 30.284672552186425 26.467534517723344 4 11.416163050337362 17.43316465220135 39.38977476821513 31.76089752924615 5 12.303013211798133 38.07496490094942 37.090372095420896 12.531649791831548 6 31.115120457267615 37.827827220765045 18.85986324423488 12.197189077732462 7 27.132011610562635 31.360835182011837 23.662527350051775 17.844625857373757 8 23.883440526691906 37.569523635755445 22.059632957776458 16.487402879776187 9 25.902258203897848 15.684169831487505 20.68284325987917 37.73072870473548 10 14.198674693314327 29.512942211169573 35.45786768162312 20.830515413892975 11 33.47255596067403 22.95354108078197 25.117833887949786 18.45606907059421 12 22.72829951258708 25.19553369061787 32.49869239923894 19.577474397556113 13 28.6278897363478 22.532856088135727 25.997431850810848 22.84182232470563 14 20.13562621379094 22.774167041890095 28.26652840251847 28.823678341800495 15 22.576586109818273 31.752062181538683 23.482869604634203 22.18848210400884 16 21.289024597321948 28.613641325441492 28.753426402095865 21.343907675140695 17 21.03524446135723 29.164954752400345 28.300937093025546 21.498863693216876 18 20.803347407199656 28.29332262001319 30.22960363242194 20.673726340365214 19 23.396132584957897 27.2455053505205 27.62788032057638 21.730481743945223 20 22.94130578149044 28.00888410488095 28.507364255880415 20.5424458577482 21 22.541247670982834 27.27887412770822 27.433358651209115 22.74651955009983 22 22.434790518567933 28.56194237460298 27.90594370873477 21.097323398094314 23 21.724259739629684 28.29052196865065 28.107077178710092 21.878141113009573 24 22.82567828164607 27.32532049338395 28.569695940619276 21.279305284350706 25 22.203810725092936 27.839093285143385 28.34514835994494 21.611947629818744 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 236.0 1 236.0 2 264.5 3 293.0 4 293.0 5 293.0 6 486.0 7 679.0 8 679.0 9 679.0 10 1054.0 11 1429.0 12 1429.0 13 1429.0 14 2441.0 15 3453.0 16 3453.0 17 3453.0 18 6092.5 19 8732.0 20 8732.0 21 8732.0 22 13571.5 23 18411.0 24 18411.0 25 18411.0 26 26321.5 27 34232.0 28 34232.0 29 34232.0 30 44422.0 31 54612.0 32 54612.0 33 54612.0 34 61415.5 35 68219.0 36 68219.0 37 68219.0 38 73344.0 39 78469.0 40 78469.0 41 78469.0 42 81478.5 43 84488.0 44 84488.0 45 84488.0 46 85459.5 47 86431.0 48 86431.0 49 86431.0 50 81558.5 51 76686.0 52 76686.0 53 76686.0 54 67371.0 55 58056.0 56 58056.0 57 58056.0 58 48877.5 59 39699.0 60 39699.0 61 39699.0 62 31814.5 63 23930.0 64 23930.0 65 23930.0 66 18355.5 67 12781.0 68 12781.0 69 12781.0 70 9206.0 71 5631.0 72 5631.0 73 5631.0 74 3893.5 75 2156.0 76 2156.0 77 2156.0 78 1463.5 79 771.0 80 771.0 81 771.0 82 500.0 83 229.0 84 229.0 85 229.0 86 145.0 87 61.0 88 61.0 89 61.0 90 38.0 91 15.0 92 15.0 93 15.0 94 8.5 95 2.0 96 2.0 97 2.0 98 3.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22434235856578538 2 0.13460541513947122 3 0.13854656468184312 4 0.07185018781093397 5 0.021827905157752087 6 0.020615243760099197 7 0.3075612469797152 8 0.30559067220852926 9 0.016825676892433902 10 0.03577351123076037 11 0.013794023398301668 12 0.015309850145367785 13 0.013187692699475222 14 0.015006684795954562 15 0.013490858048888443 16 0.018493086314206632 17 0.017128842241847127 18 0.013794023398301668 19 0.021827905157752087 20 0.015309850145367785 21 0.014097188747714892 22 0.015309850145367785 23 0.016219346193607456 24 0.01485510212124795 25 0.014248771422421504 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 659706.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.15183870711107 #Duplication Level Percentage of deduplicated Percentage of total 1 94.10957238646786 82.0182227342064 2 4.523890912316925 7.885308222376203 3 0.6673690119559142 1.7448730946431772 4 0.22906836317562873 0.7985491616153735 5 0.12633878718468866 0.5505328801586007 6 0.07199118580655507 0.37644985282479304 7 0.04820952649887504 0.2941084214303308 8 0.03351760092618262 0.23368964398143874 9 0.028810601139171596 0.22598071771823938 >10 0.13737720721518465 2.237471022652432 >50 0.01096965851639436 0.6771065710341642 >100 0.01131769353897071 1.8435786044927207 >500 0.0012188285337011533 0.7877205144712298 >1k 3.482367239146153E-4 0.3264085583948401 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1142 0.1731074145149506 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1009 0.15294691877897124 No Hit GTATCAACGCAGAGTACTTTTTTTT 918 0.13915289538066958 No Hit CTGTAGGACGTGGAATATGGCAAGA 897 0.1359696592118307 No Hit GTCCTACAGTGTGCATTTCTCATTT 887 0.1344538324647646 No Hit CTTTAGGACGTGAAATATGGCGAGG 783 0.11868923429527699 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 1.5158267470661172E-4 13 0.0 0.0 0.0 0.0 1.5158267470661172E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGTGCG 55 1.9830959E-4 13.796999 9 TAGGACC 235 0.0 13.779004 4 CCTAGAC 50 0.0014599526 13.345407 3 GTAGGAC 545 0.0 12.943121 3 GGACCTG 380 0.0 12.776 6 TGTAGGA 610 0.0 12.657822 2 GGACGTG 440 0.0 12.5482645 6 AGGACCT 370 0.0 12.349457 5 AGGACGT 435 0.0 12.254824 5 GACGTGG 220 0.0 12.115565 7 CGTGGAA 235 0.0 12.109069 9 CCTACAC 95 9.94909E-7 12.040969 3 TAGGACG 460 0.0 12.008171 4 CTGTAGG 630 0.0 11.959751 1 GGCGAGG 230 0.0 11.955364 19 TAGGACA 210 0.0 11.791275 4 GTATCAG 65 7.751906E-4 11.73849 1 GTATTGG 90 7.122524E-6 11.656973 1 TGGCGAG 505 0.0 11.453314 18 CTACACT 100 1.8669471E-6 11.428467 4 >>END_MODULE