FastQCFastQC Report
Thu 26 May 2016
SRR1926924_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926924_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659706
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15630.23692372056643413No Hit
CTGTAGGACGTGGAATATGGCAAGA11590.175684319984963No Hit
GTCCTACAGTGGACATTTCTAAATT11470.17386532788848366No Hit
GGTATCAACGCAGAGTACTTTTTTT10050.15234058808014478No Hit
CTTTAGGACGTGAAATATGGCGAGG9920.15037001330895883No Hit
GTCCTAAAGTGTGTATTTCTCATTT9900.1500668479595456No Hit
GTCCTACAGTGTGCATTTCTCATTT9640.1461256984171737No Hit
TATCAACGCAGAGTACTTTTTTTTT7980.12096297441587615No Hit
CTGTAGGACCTGGAATATGGCGAGA7260.1100490218370001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2800.015.3062564
TAGGTTG456.627554E-414.8149445
CCAACGA400.005320016814.23169219
TCCAACG759.789292E-713.91543418
CTACACC655.3163698E-513.1869284
CGGTTTC604.1363187E-412.64847713
GGACCTG4900.012.4384236
AGGACCT5650.012.3052075
GTAGGAC7900.012.177973
TGTAGGA7950.012.1115182
AAGTGTC550.003089610712.0781227
GAGCAAT802.9028812E-511.85794611
CTGTAGG7950.011.753561
AATCCCG1052.7561146E-711.74679419
GTGTAGC907.1454488E-611.6536141
GACCTGG5050.011.6510327
AGGACGT6750.011.4286715
TAGGACG6700.011.2296644
GGACGTG6750.011.1456346
GTGCAAG600.00571203411.1239041
Produced by FastQC (version 0.11.5)