##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926924_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 659706 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.76335822320852 31.0 30.0 33.0 25.0 34.0 2 30.09373721021182 31.0 30.0 34.0 26.0 34.0 3 30.139259003252963 31.0 30.0 34.0 26.0 34.0 4 33.71583857051474 35.0 35.0 37.0 28.0 37.0 5 33.65059132401403 37.0 35.0 37.0 28.0 37.0 6 33.59960649137646 37.0 35.0 37.0 28.0 37.0 7 33.585392583969224 37.0 35.0 37.0 28.0 37.0 8 33.5933855384065 37.0 35.0 37.0 28.0 37.0 9 35.12974870624187 39.0 35.0 39.0 29.0 39.0 10 35.007185018781094 39.0 35.0 39.0 28.0 39.0 11 35.04064234674234 39.0 35.0 39.0 28.0 39.0 12 34.92512725365542 39.0 35.0 39.0 27.0 39.0 13 34.944069024686755 39.0 35.0 39.0 27.0 39.0 14 36.017791258530316 40.0 36.0 41.0 27.0 41.0 15 36.01032278014752 40.0 36.0 41.0 27.0 41.0 16 35.9968470803661 40.0 36.0 41.0 27.0 41.0 17 35.96209523636286 40.0 36.0 41.0 27.0 41.0 18 35.945116460968976 40.0 36.0 41.0 27.0 41.0 19 36.0070273727994 40.0 36.0 41.0 27.0 41.0 20 35.96972893986109 40.0 36.0 41.0 27.0 41.0 21 35.91669319363474 40.0 36.0 41.0 27.0 41.0 22 35.837905066802485 40.0 36.0 41.0 27.0 41.0 23 35.75515911633364 40.0 36.0 41.0 27.0 41.0 24 35.68187192476649 40.0 36.0 41.0 27.0 41.0 25 35.566620585533556 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.14462833011735654 1101 2 -0.20740429393212167 1101 3 0.06837287530266067 1101 4 -0.14926723601467273 1101 5 -0.17889432827713136 1101 6 -0.026547903660556926 1101 7 0.03387416321870518 1101 8 0.008137799134246393 1101 9 -0.01646694020899986 1101 10 -0.012844018461208861 1101 11 -0.0064370627551397774 1101 12 -0.07969706806967025 1101 13 -0.0414212688533695 1101 14 -0.05697766565834428 1101 15 8.455613949820417E-5 1101 16 -0.09665129382280213 1101 17 -0.11177988222747359 1101 18 -0.15641187295733516 1101 19 -0.35168168218419993 1101 20 -0.17685913097137274 1101 21 -0.13073420222920618 1101 22 -0.33710621243942285 1101 23 -0.31164696673889836 1101 24 -0.21733183518853139 1101 25 -0.1685356423958453 1102 1 -0.8446283301173558 1102 2 -0.45532096059879024 1102 3 -0.3201687913640079 1102 4 -0.5503089026813441 1102 5 -0.7445193282771356 1102 6 -0.772381236993894 1102 7 -0.618209170114632 1102 8 -0.5345705341990907 1102 9 -0.5466752735423341 1102 10 -0.8003440184612103 1102 11 -0.7053953960884769 1102 12 -0.6724054014030045 1102 13 -0.5882962688533695 1102 14 -0.5944776656583457 1102 15 -0.6061654438605046 1102 16 -0.5393596271561378 1102 17 -0.6378215488941379 1102 18 -0.7376618729573394 1102 19 -0.6235566821842013 1102 20 -0.7789424643047056 1102 21 -0.736984202229209 1102 22 -0.6662728791060886 1102 23 -0.7689386334055612 1102 24 -0.6423318351885285 1102 25 -0.7872856423958439 1103 1 -0.7425724646677274 1103 2 -0.6053737834520696 1103 3 -0.48410134714493935 1103 4 -0.46419074852313 1103 5 -0.6510418942000555 1103 6 -0.5474382856754332 1103 7 -0.8943416920889362 1103 8 -0.7764167985669062 1103 9 -0.6009243438601146 1103 10 -0.6640145728898759 1103 11 -0.7173101188741455 1103 12 -0.7351335961291738 1103 13 -0.7846578307194889 1103 14 -0.6500620977070284 1103 15 -0.7696648522445457 1103 16 -0.8651329537281427 1103 17 -0.6537085502464066 1103 18 -0.7613286241405675 1103 19 -0.6523303468224668 1103 20 -0.5235925657245843 1103 21 -0.4827309060831624 1103 22 -0.5114638865435523 1103 23 -0.812054759166216 1103 24 -0.5908274403271321 1103 25 -0.7615334449651527 1104 1 -0.08635713867819206 1104 2 -0.305465256183556 1104 3 -0.160430419270984 1104 4 -0.14731977224656134 1104 5 -0.31121864576955005 1104 6 -0.15221250595243419 1104 7 -0.3756072152780945 1104 8 -0.31668736096012395 1104 9 -0.2374350898551043 1104 10 0.005295095121539362 1104 11 -0.23194473043292874 1104 12 -0.16838619007843647 1104 13 -0.3694957127360752 1104 14 -0.4549964978120329 1104 15 -0.4114116942818029 1104 16 -0.2597906180560372 1104 17 -0.32593980807850187 1104 18 -0.31395210720068434 1104 19 -0.29843239839586033 1104 20 -0.3014020194108724 1104 21 -0.45164863600069083 1104 22 -0.5396255922843878 1104 23 -0.3416952057008089 1104 24 -0.7740220947099274 1104 25 -0.6890761715498783 1105 1 0.0848397324883976 1105 2 0.1463657991304821 1105 3 0.2383654725953832 1105 4 0.36748932066890916 1105 5 -0.044979353657844 1105 6 -0.17582349587713964 1105 7 -0.18504504151902523 1105 8 -0.25278648174562335 1105 9 -0.09889079807702217 1105 10 0.047001582215614235 1105 11 -0.2418537294218126 1105 12 0.18279764428227452 1105 13 0.2164943402836883 1105 14 0.028476014544700945 1105 15 -0.01308697516338242 1105 16 0.13903504289462632 1105 17 0.13337663215492768 1105 18 -0.0403321352246806 1105 19 -0.06519162296254422 1105 20 -0.06631556075140566 1105 21 0.05170551858297756 1105 22 -0.022850713285450297 1105 23 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2209 25 0.005059318844459426 2210 1 0.035572036358107795 2210 2 9.270476867406785E-4 2210 3 0.2503259505097972 2210 4 0.06516671873357183 2210 5 0.1526902797534575 2210 6 0.2829306654917687 2210 7 0.3308666743723023 2210 8 0.13988174432225975 2210 9 0.21343267738182448 2210 10 0.313944541130887 2210 11 0.1770755248802942 2210 12 0.022288671254734993 2210 13 0.15215545198793023 2210 14 0.29964876837607335 2210 15 0.0986405631503331 2210 16 0.027478326954764043 2210 17 0.1515168035596517 2210 18 0.14986401933074234 2210 19 0.15943009283173382 2210 20 0.1503357383721564 2210 21 -0.01063979496343137 2210 22 0.08792565499206262 2210 23 0.015585745179521382 2210 24 0.07973636111484694 2210 25 0.05988612305985441 2211 1 -0.08322715540906245 2211 2 -0.14927889973471054 2211 3 0.2664028737258697 2211 4 0.11429019854855227 2211 5 0.3650567124040407 2211 6 0.08654437795407688 2211 7 0.3358747150950876 2211 8 0.2603985213039053 2211 9 0.2470264372744424 2211 10 0.12414549588758206 2211 11 0.1993950879859483 2211 12 0.02690869509652316 2211 13 0.21574228365373216 2211 14 0.2150628972555637 2211 15 0.05032206008147 2211 16 0.03317466801888713 2211 17 0.0011616188762815227 2211 18 0.13092354962228825 2211 19 0.24686865682557624 2211 20 0.2941299103405157 2211 21 0.15510129918990145 2211 22 0.23767303700869746 2211 23 0.21859093140049168 2211 24 0.056998029207562695 2211 25 0.22202924249850753 2212 1 0.13048741062338465 2212 2 0.19965589125306238 2212 3 0.02589602345080877 2212 4 0.3161262825038449 2212 5 0.025503819871012468 2212 6 -0.1620803110679674 2212 7 -0.24135731826277862 2212 8 -0.18931590456946168 2212 9 -0.2734113846534427 2212 10 -0.2084458703130565 2212 11 -0.03815002571810311 2212 12 -0.11222021621782119 2212 13 -0.18436108366817905 2212 14 -0.40176933232501 2212 15 -0.41646636978642704 2212 16 -0.15672073826724642 2212 17 -0.19742803037561885 2212 18 -0.21787020629066944 2212 19 -0.18952890440642278 2212 20 -0.046072093934334646 2212 21 -0.1479795725995814 2212 22 -0.27321732355053285 2212 23 -0.037225670442602166 2212 24 -0.15622072407741427 2212 25 -0.2016374942476986 2213 1 0.07853315221751345 2213 2 0.09980096720459031 2213 3 0.17456047130880492 2213 4 0.259973355383174 2213 5 0.19025889752931846 2213 6 0.2672933021766184 2213 7 0.12864182834927362 2213 8 0.03113127071642907 2213 9 0.09511140080482505 2213 10 0.0827685007400234 2213 11 0.1415564088270358 2213 12 0.1938388382283307 2213 13 0.11086847769040986 2213 14 0.04058857858128562 2213 15 -0.09472631175604818 2213 16 0.003583921230962517 2213 17 -0.09666851509997798 2213 18 -0.17144163486209862 2213 19 -0.010701459449947492 2213 20 0.11109209790727448 2213 21 -0.06389952480985528 2213 22 -0.1364533706575486 2213 23 0.06547956935941102 2213 24 0.3235283798652375 2213 25 0.3166122070665196 2214 1 0.34756615156271664 2214 2 0.28145015200174583 2214 3 0.23996860809980092 2214 4 0.10216540473599167 2214 5 0.39880339290249367 2214 6 0.2913503313707224 2214 7 0.19197849744569595 2214 8 0.13941014051047063 2214 9 0.23204735827178524 2214 10 -6.288710081250315E-4 2214 11 0.23875395601161387 2214 12 0.12136135462916542 2214 13 0.2359497096989145 2214 14 0.2713986748242263 2214 15 0.24151943102778972 2214 16 0.20097214228086102 2214 17 0.28454496138289187 2214 18 0.31718341301228037 2214 19 0.4431937646433255 2214 20 0.4508721022725908 2214 21 0.3333157307466621 2214 22 0.44942193769462335 2214 23 0.1684887571216933 2214 24 0.21707388420378493 2214 25 0.41743955867654137 2215 1 0.4701266045813668 2215 2 0.4992584769197599 2215 3 0.3722788934089216 2215 4 0.5143783190461733 2215 5 0.4913360813399663 2215 6 0.2650658912340731 2215 7 0.050416534842334215 2215 8 0.28565523754326705 2215 9 0.18187455578387812 2215 10 0.17207175773351224 2215 11 -0.17186263414131275 2215 12 0.03313778648657717 2215 13 0.3128669101314969 2215 14 0.3808145204502935 2215 15 0.5469855282380394 2215 16 0.5859353416773487 2215 17 0.5402273899601227 2215 18 0.5032562407262446 2215 19 0.35440599219988655 2215 20 0.18589502456439533 2215 21 0.24075678174229864 2215 22 0.37375012475261826 2215 23 0.36557272011179975 2215 24 0.3057017059215923 2215 25 0.3080579313649139 2216 1 -0.2898421700586198 2216 2 -0.18075690039320946 2216 3 -1.8901302451085016 2216 4 -1.3192791669985269 2216 5 -0.5354196586736109 2216 6 -0.11752165549609117 2216 7 -0.3110138690867288 2216 8 -0.43686036532977823 2216 9 -0.36828412082574147 2216 10 -0.36558814474608425 2216 11 -0.4101356677478023 2216 12 -0.2762600269536648 2216 13 -0.48782820717935493 2216 14 -0.559882401341163 2216 15 -0.5380808256519884 2216 16 -0.45871535402838504 2216 17 -0.38195242260926676 2216 18 -0.3675398098149003 2216 19 -0.16635220347641422 2216 20 -0.15818255241043744 2216 21 -0.11494400399131877 2216 22 -0.3135012197815712 2216 23 -0.43124590212803326 2216 24 -0.1722510716055652 2216 25 -0.21546111963520076 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17634.0 3 15.0 4 285.0 5 1965.0 6 2675.0 7 1917.0 8 1425.0 9 1290.0 10 1115.0 11 1034.0 12 1000.0 13 901.0 14 825.0 15 843.0 16 949.0 17 886.0 18 1099.0 19 1195.0 20 1630.0 21 2097.0 22 2618.0 23 3363.0 24 3760.0 25 4504.0 26 5363.0 27 5915.0 28 6854.0 29 8437.0 30 10532.0 31 13783.0 32 17581.0 33 23371.0 34 31211.0 35 43773.0 36 64142.0 37 111354.0 38 262365.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.212954489925686 19.851946952210554 10.720187934496087 28.21491062336768 2 13.806352925465726 21.051273802225285 40.89748881591327 24.244884456395717 3 18.06255495921647 25.38372903969193 29.564723005548988 26.988992995542617 4 11.81207992651272 17.949814008058134 38.43257451046375 31.805531554965395 5 12.629324262254823 38.96576971527061 35.506966266926796 12.897939755547775 6 30.871941194274566 38.612170278651895 18.085680781521155 12.430207745552384 7 27.558341217281612 32.21441913494893 22.102649006622517 18.12459064114693 8 24.50828494762215 38.59330230583812 20.05851765383624 16.839895092703486 9 26.129839652289395 15.938353225828457 20.12631342769951 37.80549369418264 10 14.575419214596035 30.00594222099605 34.26766673841797 21.150971825989938 11 33.69704778868382 23.126539583886004 24.693523325880168 18.482889301550006 12 23.034876247529194 25.402301638310515 32.03785334028595 19.524968773874342 13 28.891637600556614 22.711280682367672 25.703233121571373 22.693848595504342 14 20.585230390507288 22.931730194965635 27.76009313239534 28.72294628213174 15 22.497453341579657 31.78851214513528 23.36634894071137 22.347685572573702 16 21.44725952020761 28.992939236200588 28.229412674207445 21.330388569384358 17 21.033643825649342 29.45937957117196 28.063755769624304 21.443220833554392 18 20.99293166773533 28.33881307194298 29.97515548663565 20.69309977368604 19 23.032788997473105 27.44992822527713 27.672452603671655 21.84483017357811 20 22.98504023780398 28.04868251319154 28.299704564023507 20.66657268498097 21 22.450180026530226 27.31612658707599 27.389956414629523 22.84373697176426 22 22.624926861359455 28.689446511021576 27.384016516448234 21.30161011117073 23 21.68966175841966 28.46343674303402 27.92774357062085 21.91915792792547 24 22.50047748542533 27.452077137391505 28.650035015597858 21.39741036158531 25 22.1949526125263 27.982257741773136 28.21373781963048 21.609051826070083 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 252.0 1 252.0 2 251.0 3 250.0 4 250.0 5 250.0 6 445.5 7 641.0 8 641.0 9 641.0 10 1043.5 11 1446.0 12 1446.0 13 1446.0 14 2368.5 15 3291.0 16 3291.0 17 3291.0 18 5769.0 19 8247.0 20 8247.0 21 8247.0 22 12870.0 23 17493.0 24 17493.0 25 17493.0 26 25042.5 27 32592.0 28 32592.0 29 32592.0 30 42605.5 31 52619.0 32 52619.0 33 52619.0 34 59773.5 35 66928.0 36 66928.0 37 66928.0 38 71789.5 39 76651.0 40 76651.0 41 76651.0 42 80289.0 43 83927.0 44 83927.0 45 83927.0 46 85871.0 47 87815.0 48 87815.0 49 87815.0 50 83440.5 51 79066.0 52 79066.0 53 79066.0 54 69315.0 55 59564.0 56 59564.0 57 59564.0 58 50407.5 59 41251.0 60 41251.0 61 41251.0 62 33184.0 63 25117.0 64 25117.0 65 25117.0 66 19113.5 67 13110.0 68 13110.0 69 13110.0 70 9573.0 71 6036.0 72 6036.0 73 6036.0 74 4163.0 75 2290.0 76 2290.0 77 2290.0 78 1544.0 79 798.0 80 798.0 81 798.0 82 517.5 83 237.0 84 237.0 85 237.0 86 151.0 87 65.0 88 65.0 89 65.0 90 41.5 91 18.0 92 18.0 93 18.0 94 10.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11414175405407864 2 0.111261683234653 3 0.01909941701303308 4 0.0 5 0.004092732217078517 6 0.33636195517397144 7 0.02091840910951242 8 0.011520283277702492 9 0.011671865952409103 10 0.0030316534941322347 11 0.004699062915904963 12 3.031653494132235E-4 13 4.547480241198352E-4 14 0.0 15 0.002728488144719011 16 6.06330698826447E-4 17 1.5158267470661175E-4 18 4.547480241198352E-4 19 4.547480241198352E-4 20 4.547480241198352E-4 21 0.01227819665123555 22 0.0 23 0.0 24 0.0 25 0.005760141638851246 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 659706.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.56949618759595 #Duplication Level Percentage of deduplicated Percentage of total 1 92.56313447887825 78.28017648423425 2 5.715595869253962 9.66730126149424 3 0.8705390147235993 2.2086313766046284 4 0.3200540040661579 1.0826722350679103 5 0.14310263519933802 0.6051058880962675 6 0.08139617515960873 0.41301801149072526 7 0.05778716928676634 0.34209222548824025 8 0.04298067008589531 0.2907882891975554 9 0.021228717795854977 0.1615771771833697 >10 0.1558384812210742 2.4747427476744046 >50 0.013812762594855274 0.814204229795856 >100 0.012736191891743942 2.166019519059298 >500 0.0010762979057855856 0.754268738323894 >1k 7.175319371903904E-4 0.7394018162893523 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1563 0.23692372056643413 No Hit CTGTAGGACGTGGAATATGGCAAGA 1159 0.175684319984963 No Hit GTCCTACAGTGGACATTTCTAAATT 1147 0.17386532788848366 No Hit GGTATCAACGCAGAGTACTTTTTTT 1005 0.15234058808014478 No Hit CTTTAGGACGTGAAATATGGCGAGG 992 0.15037001330895883 No Hit GTCCTAAAGTGTGTATTTCTCATTT 990 0.1500668479595456 No Hit GTCCTACAGTGTGCATTTCTCATTT 964 0.1461256984171737 No Hit TATCAACGCAGAGTACTTTTTTTTT 798 0.12096297441587615 No Hit CTGTAGGACCTGGAATATGGCGAGA 726 0.1100490218370001 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 280 0.0 15.306256 4 TAGGTTG 45 6.627554E-4 14.814944 5 CCAACGA 40 0.0053200168 14.231692 19 TCCAACG 75 9.789292E-7 13.915434 18 CTACACC 65 5.3163698E-5 13.186928 4 CGGTTTC 60 4.1363187E-4 12.648477 13 GGACCTG 490 0.0 12.438423 6 AGGACCT 565 0.0 12.305207 5 GTAGGAC 790 0.0 12.17797 3 TGTAGGA 795 0.0 12.111518 2 AAGTGTC 55 0.0030896107 12.078122 7 GAGCAAT 80 2.9028812E-5 11.857946 11 CTGTAGG 795 0.0 11.75356 1 AATCCCG 105 2.7561146E-7 11.746794 19 GTGTAGC 90 7.1454488E-6 11.653614 1 GACCTGG 505 0.0 11.651032 7 AGGACGT 675 0.0 11.428671 5 TAGGACG 670 0.0 11.229664 4 GGACGTG 675 0.0 11.145634 6 GTGCAAG 60 0.005712034 11.123904 1 >>END_MODULE