FastQCFastQC Report
Thu 26 May 2016
SRR1926923_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926923_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656140
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11840.1804492943579114No Hit
GTATCAACGCAGAGTACTTTTTTTT9850.15012040113390435No Hit
GTCCTAAAGTGTGTATTTCTCATTT9300.1417380437101838No Hit
CTGTAGGACGTGGAATATGGCAAGA9300.1417380437101838No Hit
GTCCTACAGTGTGCATTTCTCATTT8850.13487975127259424No Hit
CTTTAGGACGTGAAATATGGCGAGG8820.13442253177675495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3100.012.9022414
GGCGAGG3050.012.74589919
TTAGACT853.8281487E-612.323994
AGGACCT4200.012.0098675
TTAGGAC4350.011.8407953
ATTAGAC657.7457144E-411.7395913
GGACCTG4100.011.6064066
TGGCGAG7100.011.35133718
AGGACGT6250.011.2684295
TAGGACA2800.011.2236324
ACCTCTC855.263408E-511.1864998
GTCCTAT1201.18840035E-711.1366941
GACAACT600.005790743511.1034627
AGGACTA600.005790743511.1034625
ACTGTCT951.3433322E-511.0098698
CTGTAGG7550.010.9997241
TTTAGGA4600.010.9890732
GTAGGAC7350.010.9010483
GAACCAT700.001467024310.8768616
GGACGTG6150.010.8326466
Produced by FastQC (version 0.11.5)