##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1926923_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	656140
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.703083183466944	31.0	28.0	31.0	16.0	34.0
2	27.869280946139543	31.0	28.0	31.0	16.0	34.0
3	27.48776023409638	31.0	27.0	31.0	10.0	34.0
4	31.155104093638553	35.0	32.0	37.0	16.0	37.0
5	31.24143627884293	35.0	32.0	37.0	16.0	37.0
6	30.7263846130399	35.0	32.0	37.0	10.0	37.0
7	30.66166366933886	35.0	32.0	37.0	10.0	37.0
8	30.2500441978846	35.0	30.0	37.0	10.0	37.0
9	32.39677812661932	37.0	32.0	39.0	11.0	39.0
10	32.587397506629685	37.0	32.0	39.0	10.0	39.0
11	32.760942786600424	37.0	33.0	39.0	10.0	39.0
12	32.63091108604871	37.0	33.0	39.0	10.0	39.0
13	32.75342762215381	38.0	33.0	39.0	10.0	39.0
14	33.72525985308013	39.0	33.0	40.0	10.0	41.0
15	33.28762306824763	39.0	33.0	40.0	8.0	41.0
16	33.386175206510806	39.0	33.0	40.0	8.0	41.0
17	33.615428109854605	39.0	33.0	41.0	2.0	41.0
18	33.67144664248484	39.0	33.0	41.0	2.0	41.0
19	33.06104642301948	39.0	32.0	40.0	2.0	41.0
20	33.31512786905234	39.0	33.0	41.0	2.0	41.0
21	33.291454567622765	39.0	33.0	41.0	2.0	41.0
22	33.31164538055903	39.0	33.0	41.0	2.0	41.0
23	33.298021763648	39.0	33.0	41.0	2.0	41.0
24	32.02740878471058	38.0	31.0	40.0	2.0	41.0
25	32.57950894626147	39.0	32.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.24315349726063218
1101	2	0.01070128984641272
1101	3	-0.9001478323456418
1101	4	-0.28408116703585407
1101	5	0.12504783532909158
1101	6	-1.6178457202260823
1101	7	-0.02358070089887221
1101	8	0.3723653444042867
1101	9	0.21239329896293668
1101	10	-8.9400366116171
1101	11	-3.162659224303301
1101	12	-0.8515410342736978
1101	13	-0.2123038461087674
1101	14	-0.03391173514015122
1101	15	-1.3073262791401454
1101	16	0.2484727072895936
1101	17	0.5263777393802869
1101	18	0.4156484235963944
1101	19	-0.24527233375672353
1101	20	0.33448363633739575
1101	21	0.5354388142565369
1101	22	0.8314656177286253
1101	23	0.673111014402366
1101	24	0.7262388972127525
1101	25	0.9134731711625435
1102	1	-0.27308799933801
1102	2	-0.38929871015358586
1102	3	-0.991878274522513
1102	4	-0.5992809969678277
1102	5	-0.2713382190926765
1102	6	-1.2084707202260851
1102	7	-0.3934361430757427
1102	8	-0.13917802294265513
1102	9	-0.2728532996765196
1102	10	-0.07562334631097656
1102	11	-0.2885308229427537
1102	12	-0.3470129730492104
1102	13	-0.5569892202584299
1102	14	-0.8008335038476346
1102	15	-0.17231352403810263
1102	16	-0.2832874967920418
1102	17	-0.6614198796673278
1102	18	-0.867663651233535
1102	19	-0.4522026058655655
1102	20	-0.5960223160435518
1102	21	-0.5621802333625112
1102	22	-0.5795462870332813
1102	23	-1.0790998699513779
1102	24	-4.306222837481123
1102	25	-2.9704341417626274
1103	1	-0.49585813185584726
1103	2	-0.868911350316683
1103	3	-0.17088885046542757
1103	4	-0.3573945290779186
1103	5	-0.7744154362180566
1103	6	0.26992186387177597
1103	7	0.04303811788246392
1103	8	0.04856726248038257
1103	9	-0.15927659223513757
1103	10	-0.2098040292875325
1103	11	-0.35288161295294884
1103	12	-0.23361784053137313
1103	13	-0.2577218910025678
1103	14	-0.3811979279047222
1103	15	0.031123657409395378
1103	16	0.086723971097868
1103	17	-0.08237426294969197
1103	18	-0.2562180600001014
1103	19	-1.7537507710031797
1103	20	-0.49088406425966014
1103	21	-0.398163923474641
1103	22	-0.3389435857081011
1103	23	-0.5297369749462817
1103	24	0.9344531309184703
1103	25	0.1922474331609223
1104	1	-0.1704481153393722
1104	2	-0.20864364362339316
1104	3	-1.1797887572928971
1104	4	-0.37849137738406924
1104	5	-0.09898450022539507
1104	6	-1.1641097939210674
1104	7	0.08383057476799749
1104	8	0.5637038859621555
1104	9	0.3340150899515919
1104	10	0.5081983015907596
1104	11	0.22713042748372203
1104	12	0.4252778406662472
1104	13	0.24590015196947945
1104	14	0.004024053300092589
1104	15	0.5298710972515579
1104	16	-0.08415110989336938
1104	17	0.026436227463342732
1104	18	-0.04872920053411889
1104	19	-1.0882078088679528
1104	20	-0.21509963607084615
1104	21	-0.018713839640234653
1104	22	0.015205930639872633
1104	23	-0.08023429506226165
1104	24	1.1276979659989195
1104	25	0.39511835738442613
1105	1	0.25159729774154727
1105	2	0.16306785580814775
1105	3	0.8181198372599638
1105	4	0.1993374572114277
1105	5	0.2933709954088357
1105	6	0.10793474301661377
1105	7	0.5208198992203279
1105	8	0.6478971281147494
1105	9	-0.17019091296957356
1105	10	0.3325274593284959
1105	11	0.2130647208638905
1105	12	0.26867496753487785
1105	13	0.21043902747570797
1105	14	-0.04593672640554303
1105	15	0.6435821204734751
1105	16	0.11173390300655228
1105	17	-0.0410082482473868
1105	18	0.05783043235155816
1105	19	-0.5570805011324325
1105	20	0.04655384206319013
1105	21	0.06893759141090072
1105	22	-0.012671916439117581
1105	23	-0.11331437146195356
1105	24	-4.509052016735907
1105	25	-2.097394740956986
1106	1	-0.3149562789078999
1106	2	-1.0415164520890698
1106	3	-0.4779333820493932
1106	4	-0.8173925561076132
1106	5	-0.5407602621034293
1106	6	-0.15232152667769583
1106	7	-3.2733420570542364
1106	8	-0.9519804422974936
1106	9	-1.1457699663431882
1106	10	-0.29961528179484986
1106	11	-0.6468305541255503
1106	12	-0.23340410208146523
1106	13	-0.5143816933767127
1106	14	-1.0069490952454885
1106	15	-0.2224143304897197
1106	16	-0.161916529050103
1106	17	-0.6573204173017402
1106	18	-0.9081542426313902
1106	19	-0.29144991769351947
1106	20	-0.2574806493768875
1106	21	-0.5586856097065933
1106	22	-0.8510516633773619
1106	23	-0.914516536618045
1106	24	-4.068017192319832
1106	25	-2.4507096793970327
1107	1	0.31258597144552525
1107	2	0.2830120468185271
1107	3	0.6881723562875806
1107	4	0.5743951318502347
1107	5	0.48564219259391095
1107	6	0.40267220806076764
1107	7	-1.466336209476804
1107	8	-1.2456645767275134
1107	9	-0.08600901016789209
1107	10	0.10736968157746674
1107	11	-0.07759539797595494
1107	12	0.04724748511812038
1107	13	-0.0632226199928283
1107	14	0.09108216680309766
1107	15	-1.3785759742373074
1107	16	-0.38520482746933027
1107	17	0.06476703932337102
1107	18	-0.14553134711667326
1107	19	0.5260261457944608
1107	20	0.5053430558023919
1107	21	0.11238815973177907
1107	22	0.11322429729607109
1107	23	0.09656414067278973
1107	24	-3.5773508255289315
1107	25	-1.7225216251623614
1108	1	0.4576027577908022
1108	2	-0.019092220478068356
1108	3	0.49799697129853016
1108	4	0.801410374713587
1108	5	0.6925813974264976
1108	6	-2.6195173033135966
1108	7	-0.9782874213451613
1108	8	0.5032414706659978
1108	9	0.18535097760961605
1108	10	0.4859732613586374
1108	11	0.4219012075390509
1108	12	0.6467462452398749
1108	13	0.6190247915409799
1108	14	0.5438198884827514
1108	15	-0.500327904569076
1108	16	0.5552633881637163
1108	17	0.3367303169895024
1108	18	0.5506162160231014
1108	19	0.6797602751570508
1108	20	-0.6727645726807197
1108	21	0.1273969544447695
1108	22	0.238208137745481
1108	23	0.655634643322955
1108	24	-0.8169744353178992
1108	25	-0.152731953955346
1109	1	0.21557479135966418
1109	2	0.3316315224045532
1109	3	-1.464140871421737
1109	4	-0.0969311907662771
1109	5	0.11591953284530732
1109	6	0.24533257434756095
1109	7	0.2965347871568156
1109	8	0.8233462018635471
1109	9	0.07419127396688907
1109	10	0.4433204521386358
1109	11	0.3028209212432884
1109	12	-0.2076476629716879
1109	13	-0.023219840413467807
1109	14	0.18434091475701564
1109	15	-1.3499540279140732
1109	16	-0.21579718671451786
1109	17	-0.2252280192022127
1109	18	-0.1311084186753959
1109	19	-1.6992471795089763
1109	20	-0.536308168756733
1109	21	-0.34241279150204207
1109	22	0.03116107730780726
1109	23	-0.10565025754827673
1109	24	-1.0427296204268615
1109	25	-0.3425029402122348
1110	1	0.05755546057760341
1110	2	0.1876243667694908
1110	3	0.5048608118130922
1110	4	0.18499460348656882
1110	5	0.3825424203101164
1110	6	0.4398952729579477
1110	7	0.4823529676026084
1110	8	0.9367683013027204
1110	9	0.6743371417386044
1110	10	0.5583408698526071
1110	11	0.3508912396992443
1110	12	0.41679849238408906
1110	13	0.16151329515864177
1110	14	-0.30102418869022074
1110	15	0.310893024160535
1110	16	0.1912682480959873
1110	17	-0.13450694555503162
1110	18	-0.12451511991253739
1110	19	-2.1453846084621233
1110	20	-0.4680758295132037
1110	21	-0.47490337519957393
1110	22	-0.22019664406021633
1110	23	-0.20781619581960342
1110	24	-1.1713914109962182
1110	25	-1.041302974933675
1111	1	0.7563043281926163
1111	2	0.4675678275640145
1111	3	1.029025577830808
1111	4	0.9183346542249531
1111	5	0.9865881187538719
1111	6	0.9436629838358108
1111	7	1.0606217228172277
1111	8	1.5088454296685647
1111	9	1.044268718376351
1111	10	0.9036198838631009
1111	11	0.6208602956903846
1111	12	0.8726981004517569
1111	13	0.709383281353432
1111	14	0.8808476695888601
1111	15	-0.10321898825473852
1111	16	0.31087352835302795
1111	17	0.3367949817124227
1111	18	0.635711187580128
1111	19	1.1308043251466842
1111	20	0.5104714782826889
1111	21	0.9061627711507043
1111	22	1.0323774718319711
1111	23	0.8397750494554543
1111	24	1.6081932650333854
1111	25	1.3968268995010718
1112	1	0.09638689738903139
1112	2	0.358556389941743
1112	3	-1.4999464743954078
1112	4	-0.18828341991031294
1112	5	-0.05109076119626721
1112	6	0.8417348326814462
1112	7	0.8366301899398287
1112	8	0.9342639455988149
1112	9	0.14665138732697613
1112	10	0.8013487699902626
1112	11	0.7220278440416763
1112	12	0.5456020174179059
1112	13	0.22210793576317656
1112	14	0.32599760514505505
1112	15	0.7702120955996463
1112	16	0.7731531609772659
1112	17	0.05848995509593635
1112	18	0.45952792010424304
1112	19	1.0431957894315431
1112	20	0.5848989979062367
1112	21	0.5481788387696795
1112	22	0.6419245582161679
1112	23	0.5839045787299071
1112	24	1.7422667955026725
1112	25	0.9229593679291455
1113	1	-0.5688203696393437
1113	2	0.20855776701323592
1113	3	-4.5707119573432635
1113	4	-1.3209851364521406
1113	5	-0.11794624953691368
1113	6	0.6027507383014097
1113	7	0.7004031703756404
1113	8	1.2185252622390799
1113	9	0.5865226711218625
1113	10	0.6045199435305904
1113	11	0.36151674617808993
1113	12	0.24350718072525268
1113	13	-0.2669399037004965
1113	14	-0.7039594746460196
1113	15	0.28615280867391846
1113	16	0.26261767562175464
1113	17	0.39751340706768445
1113	18	0.29692065289818004
1113	19	0.9979585466662755
1113	20	0.330036824994032
1113	21	0.29929848673690174
1113	22	0.6246223212301558
1113	23	0.40243942796723076
1113	24	1.721587623842268
1113	25	1.0853391962305423
1114	1	0.5296769421284111
1114	2	0.44434269465232745
1114	3	0.593486405083155
1114	4	0.34819089151029914
1114	5	0.6305063588494058
1114	6	0.951285518221237
1114	7	1.1113659210031237
1114	8	1.1580551101442218
1114	9	0.4988695900338911
1114	10	0.49853330803283313
1114	11	0.11272649375472099
1114	12	-0.4322890821065073
1114	13	-0.03635228248887046
1114	14	0.09995978019426133
1114	15	-2.114186213502059
1114	16	-0.16363638773474065
1114	17	-0.06783931897724926
1114	18	0.31555222985087283
1114	19	0.6545711156378218
1114	20	0.6672536389780106
1114	21	0.12679080791906472
1114	22	0.25523113132778263
1114	23	-0.22742088042581088
1114	24	0.8319067835909664
1114	25	0.44647705663540194
1115	1	-3.777440539610623
1115	2	-1.2731277064361137
1115	3	-0.5389081691941335
1115	4	0.025064573044563332
1115	5	0.41235911671897085
1115	6	0.9271356924133229
1115	7	0.5906329399478025
1115	8	0.9541421443435922
1115	9	0.37374676228134973
1115	10	-0.8458138667570729
1115	11	-0.028257433847343805
1115	12	-0.49230804739865164
1115	13	0.10527069300055558
1115	14	-0.18635085205085744
1115	15	0.22507262837824982
1115	16	-0.22350589827902922
1115	17	-0.22718041250499965
1115	18	-0.2671563711839724
1115	19	-2.080277265097287
1115	20	-0.935803140083209
1115	21	-0.7461331453823377
1115	22	-0.5639406488672307
1115	23	-0.7557107745362615
1115	24	-2.5044454211242453
1115	25	-1.6043289682805941
1116	1	0.04443483171221985
1116	2	-0.7358740526193408
1116	3	-0.02676697315265031
1116	4	-0.47024275495869716
1116	5	0.07395287679773332
1116	6	0.5122912660752874
1116	7	0.34865860578270613
1116	8	-2.102776767234893
1116	9	-0.6852676832381661
1116	10	-0.9334715198269592
1116	11	-0.2794982886961819
1116	12	-0.1364678817250109
1116	13	-0.5281364805324031
1116	14	-0.3499773394640755
1116	15	-1.1783494829422168
1116	16	-0.4480420630020845
1116	17	-0.43193928606002174
1116	18	-0.32738111698696315
1116	19	0.2460565265545256
1116	20	-0.12565987312117954
1116	21	-0.2703994114446999
1116	22	0.011635219816035658
1116	23	0.17695035835912165
1116	24	1.079379331468651
1116	25	0.4476451961369179
1201	1	0.680388646097235
1201	2	0.3453085085300671
1201	3	0.9827435407641119
1201	4	0.7265085465565875
1201	5	0.7356548806950087
1201	6	1.2449992903896288
1201	7	1.013132328261836
1201	8	1.3973599282250753
1201	9	0.7241875708118002
1201	10	0.7917996897824437
1201	11	0.6580522449977977
1201	12	0.8884368677151215
1201	13	0.6805771279368997
1201	14	0.8367195747086242
1201	15	1.2715094589767588
1201	16	0.8967416964139048
1201	17	0.6315339419887209
1201	18	0.4940335356029806
1201	19	0.3195463325633128
1201	20	0.7252024716422198
1201	21	0.9184991721576594
1201	22	0.9317992543679949
1201	23	1.008320512214226
1201	24	-0.07504582049598696
1201	25	0.28592865016943136
1202	1	0.5411663866269016
1202	2	0.44228023721483467
1202	3	0.19870286582836627
1202	4	0.14393172233041795
1202	5	0.45052581599504293
1202	6	1.126364806089704
1202	7	0.9705331551698713
1202	8	1.3727627665310322
1202	9	1.0789668757620774
1202	10	1.0707582326363934
1202	11	0.8449669840747873
1202	12	1.304796237477106
1202	13	0.9378572709696371
1202	14	1.0393857943979796
1202	15	1.4310088443829514
1202	16	1.2655940821553315
1202	17	0.8322424010344278
1202	18	0.8104174891173415
1202	19	0.618433359046719
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2211	2	0.34312865629440026
2211	3	-0.0022412470311046206
2211	4	0.42812547757137764
2211	5	0.3991885694109172
2211	6	-2.360769806449138
2211	7	-0.14662069477833484
2211	8	0.38530358436756273
2211	9	0.4730210364684311
2211	10	0.26161306418574526
2211	11	0.3528888505967629
2211	12	0.18865654428912393
2211	13	0.12762691532419979
2211	14	0.42734909534011933
2211	15	0.8966912791483992
2211	16	0.20557731108050348
2211	17	0.16353130746169597
2211	18	0.18060739687642524
2211	19	0.6430976909166901
2211	20	0.08363345559030932
2211	21	0.09289630521918468
2211	22	-2.794508408245399
2211	23	-1.1370739405543162
2211	24	0.7632212815441832
2211	25	0.4528798616738001
2212	1	-0.7432621989613608
2212	2	-0.21321584763004608
2212	3	-2.851135572074302
2212	4	-1.5962030779471128
2212	5	-0.5148998951755388
2212	6	0.185167462448117
2212	7	0.3671453070184185
2212	8	0.004224754835124855
2212	9	0.10611327282818905
2212	10	0.19307957270973475
2212	11	0.2555428587898234
2212	12	0.07593311922951074
2212	13	0.19963742757584413
2212	14	-0.1896517712675987
2212	15	-1.094906508972194
2212	16	-0.20010717664138156
2212	17	-0.10095613202138054
2212	18	-0.03094637251413701
2212	19	0.7452903319875404
2212	20	-0.6092344158552336
2212	21	-0.8552122484284226
2212	22	-0.15258065614815308
2212	23	0.036107286357470514
2212	24	1.2141573414265991
2212	25	0.5473330427948113
2213	1	-0.9486636321528898
2213	2	-1.0201312810593492
2213	3	-0.30221012112208356
2213	4	-6.969356667904091
2213	5	-2.1157591553537323
2213	6	-3.9081476415435574
2213	7	-1.7912880033990106
2213	8	-3.50411111992344
2213	9	-1.3838760962608454
2213	10	-1.0439044533558075
2213	11	-0.9043997997649562
2213	12	-0.9259785494444515
2213	13	-0.8277901504200642
2213	14	-0.9476503687454709
2213	15	-3.739905587167108
2213	16	-1.7381079450994505
2213	17	-1.181751916873793
2213	18	-1.1721057448596426
2213	19	-0.7176970401443086
2213	20	-2.055713723484846
2213	21	-1.115672151630278
2213	22	-1.1844277295462433
2213	23	-0.95114165098218
2213	24	-0.7383791732542271
2213	25	-0.6217845915948921
2214	1	0.2673399285899194
2214	2	0.17376435290947612
2214	3	0.22453501376577734
2214	4	0.3846998588880268
2214	5	0.4695964655920086
2214	6	0.2929650905847261
2214	7	0.683534577644977
2214	8	-2.308688158077789
2214	9	-1.1417159122891327
2214	10	-0.580071544509174
2214	11	-0.5510026697896038
2214	12	-1.6148339189951564
2214	13	-0.7773157968350084
2214	14	-0.5577028732170035
2214	15	-2.3374880735876538
2214	16	-0.9711816409361802
2214	17	-0.5476248346222832
2214	18	-0.5051918043866905
2214	19	0.549993340080384
2214	20	-1.3629423610885993
2214	21	-0.507225278407553
2214	22	-0.33095394469093975
2214	23	-0.7865323023838116
2214	24	-1.711994233877519
2214	25	-0.7053722048256894
2215	1	0.5231957844457753
2215	2	0.21340399254911802
2215	3	0.7569674461982103
2215	4	0.8036187778069497
2215	5	0.9443712403667845
2215	6	1.4389110365306728
2215	7	1.1493003434107436
2215	8	0.3030235536339241
2215	9	0.7970228264496058
2215	10	0.545153680716048
2215	11	0.46431264552571605
2215	12	0.0761570719958371
2215	13	0.46863014911093615
2215	14	0.533288117773985
2215	15	0.3462957101961308
2215	16	0.6189084491539063
2215	17	0.24516795817050507
2215	18	-0.4880746872695738
2215	19	0.67702036710741
2215	20	-2.9467261448723825
2215	21	-1.757675728858004
2215	22	-0.626449440186434
2215	23	-0.5730187888703
2215	24	-0.5957780176613028
2215	25	-0.13690373635721897
2216	1	-0.837378999930074
2216	2	-1.4550180707219695
2216	3	-0.6436230199339974
2216	4	-0.935395191757646
2216	5	-1.0867874494064722
2216	6	-2.634364370314895
2216	7	-10.175848073211917
2216	8	-11.102149825784572
2216	9	-6.441063039167343
2216	10	-3.8731699715330024
2216	11	-2.868513430994856
2216	12	-2.5498826444524063
2216	13	-2.3810826187190592
2216	14	-2.3904057359376303
2216	15	-3.310141010716613
2216	16	-2.360430036756515
2216	17	-2.778341874930799
2216	18	-2.5201854836787696
2216	19	-2.143436324042
2216	20	-3.0643745214905636
2216	21	-5.525116882267188
2216	22	-4.069092616222147
2216	23	-4.109398863438649
2216	24	-4.107072082596577
2216	25	-3.5965424158893278
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	46458.0
3	8.0
4	391.0
5	2043.0
6	2747.0
7	2232.0
8	1702.0
9	1546.0
10	1502.0
11	1367.0
12	1336.0
13	1393.0
14	1298.0
15	1435.0
16	1444.0
17	1541.0
18	1824.0
19	2268.0
20	2895.0
21	3822.0
22	4647.0
23	5418.0
24	6262.0
25	7236.0
26	8174.0
27	8857.0
28	9981.0
29	11314.0
30	13660.0
31	16477.0
32	20942.0
33	27580.0
34	36793.0
35	51074.0
36	73304.0
37	116642.0
38	158527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.34915785051369	19.22438223274644	13.110644311194285	25.315815605545584
2	13.54944065442669	20.350869160447477	43.42215710513865	22.67753307998718
3	18.30841245591088	25.33291005743223	30.536914288156265	25.821763198500626
4	11.26200117438021	17.903502550845324	39.711590522599195	31.122905752175274
5	12.144928154830685	38.36285087973366	37.08540017134303	12.40682079409263
6	30.735788196413438	37.975378198913425	19.086164108755437	12.202669495917707
7	26.573003218088566	31.764903724880185	23.883567874150568	17.778525182880685
8	23.72371454253062	37.65279043175717	22.270103521441108	16.3533915042711
9	25.851640478760054	15.845207826495212	20.981878822245392	37.32127287249934
10	14.223794241631918	29.833286325209823	35.45962510386254	20.483294329295717
11	33.24604177495545	23.21294923015248	25.30108728292068	18.23992171197139
12	22.591595583169216	25.376808589772516	32.64424513288905	19.38735069416922
13	28.30657183533981	22.910270988747673	26.18414674670219	22.599010429210324
14	19.965763917363912	23.275201095066862	28.382261258198906	28.37677372937032
15	22.506569681301862	31.939473571894567	23.66137027394504	21.89258647285853
16	20.87339200051218	29.026534373347033	29.058698173980247	21.041375452160537
17	20.790944261355495	29.48892269513708	28.49704127580309	21.22309176770433
18	20.54861755753	28.51860150689964	30.56570469583827	20.367076239732093
19	23.257884338642405	27.450059757555064	27.846563330812945	21.445492572989586
20	22.83841563331225	28.283704375528746	28.687646238386673	20.19023375277234
21	22.29831795075033	27.454214268838268	27.774161833410815	22.47330594700059
22	22.060984458169976	29.016852733691444	28.040229499600635	20.881933308537945
23	21.53831145174542	28.608404176022166	28.250799123821892	21.602485248410517
24	22.67940979208585	27.79373208950674	28.613194317419673	20.913663800987745
25	22.020730127276885	27.942382440362778	28.59309503848792	21.443792393872418
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	221.0
1	221.0
2	289.5
3	358.0
4	358.0
5	358.0
6	567.0
7	776.0
8	776.0
9	776.0
10	1128.5
11	1481.0
12	1481.0
13	1481.0
14	2545.0
15	3609.0
16	3609.0
17	3609.0
18	6333.5
19	9058.0
20	9058.0
21	9058.0
22	14053.0
23	19048.0
24	19048.0
25	19048.0
26	27549.5
27	36051.0
28	36051.0
29	36051.0
30	46497.0
31	56943.0
32	56943.0
33	56943.0
34	63877.0
35	70811.0
36	70811.0
37	70811.0
38	75021.0
39	79231.0
40	79231.0
41	79231.0
42	82183.0
43	85135.0
44	85135.0
45	85135.0
46	85482.0
47	85829.0
48	85829.0
49	85829.0
50	80574.0
51	75319.0
52	75319.0
53	75319.0
54	65090.0
55	54861.0
56	54861.0
57	54861.0
58	45818.5
59	36776.0
60	36776.0
61	36776.0
62	29211.0
63	21646.0
64	21646.0
65	21646.0
66	16453.0
67	11260.0
68	11260.0
69	11260.0
70	8079.0
71	4898.0
72	4898.0
73	4898.0
74	3409.5
75	1921.0
76	1921.0
77	1921.0
78	1271.0
79	621.0
80	621.0
81	621.0
82	414.0
83	207.0
84	207.0
85	207.0
86	134.0
87	61.0
88	61.0
89	61.0
90	36.5
91	12.0
92	12.0
93	12.0
94	8.5
95	5.0
96	5.0
97	5.0
98	3.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.23851617032950284
2	0.13868991373792178
3	0.1421952632060231
4	0.0723930868412229
5	0.022251348797512727
6	0.020727283811381718
7	0.30862315969152926
8	0.3011552412594873
9	0.0170695278446673
10	0.034291462187947694
11	0.015393056359923185
12	0.01585027585576249
13	0.014935836864083885
14	0.01630749535160179
15	0.014631023866857681
16	0.018745999329411407
17	0.017374340841893498
18	0.013868991373792178
19	0.023775413783643735
20	0.016002682354375592
21	0.014478617368244583
22	0.01585027585576249
23	0.016612308348827994
24	0.015240649861310088
25	0.013716584875179077
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	656140.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.39455735520262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.0163817710248	81.22503687245427
2	4.5549747106568885	7.87050047782688
3	0.7065756788556443	1.8313287903805555
4	0.2346000159885374	0.8107265814741259
5	0.13061518483252443	0.5642220538736962
6	0.06951656868339029	0.36035119081524264
7	0.05028707732223085	0.30411708501586876
8	0.03425052481587921	0.2367247144521013
9	0.030603916449471406	0.23796106334889175
>10	0.14286693642787554	2.291534568546575
>50	0.01572400455609064	0.9265020691366268
>100	0.011836907739359113	2.107410640717891
>500	0.0015900323825462092	1.0528658360209253
>1k	1.766702647273566E-4	0.1807180559363258
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	1184	0.1804492943579114	No Hit
GTATCAACGCAGAGTACTTTTTTTT	985	0.15012040113390435	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	930	0.1417380437101838	No Hit
CTGTAGGACGTGGAATATGGCAAGA	930	0.1417380437101838	No Hit
GTCCTACAGTGTGCATTTCTCATTT	885	0.13487975127259424	No Hit
CTTTAGGACGTGAAATATGGCGAGG	882	0.13442253177675495	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	310	0.0	12.902241	4
GGCGAGG	305	0.0	12.745899	19
TTAGACT	85	3.8281487E-6	12.32399	4
AGGACCT	420	0.0	12.009867	5
TTAGGAC	435	0.0	11.840795	3
ATTAGAC	65	7.7457144E-4	11.739591	3
GGACCTG	410	0.0	11.606406	6
TGGCGAG	710	0.0	11.351337	18
AGGACGT	625	0.0	11.268429	5
TAGGACA	280	0.0	11.223632	4
ACCTCTC	85	5.263408E-5	11.186499	8
GTCCTAT	120	1.18840035E-7	11.136694	1
GACAACT	60	0.0057907435	11.103462	7
AGGACTA	60	0.0057907435	11.103462	5
ACTGTCT	95	1.3433322E-5	11.009869	8
CTGTAGG	755	0.0	10.999724	1
TTTAGGA	460	0.0	10.989073	2
GTAGGAC	735	0.0	10.901048	3
GAACCAT	70	0.0014670243	10.876861	6
GGACGTG	615	0.0	10.832646	6
>>END_MODULE