FastQCFastQC Report
Thu 26 May 2016
SRR1926923_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926923_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656140
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16770.25558569817417015No Hit
CTGTAGGACGTGGAATATGGCAAGA11620.1770963513884232No Hit
GTCCTACAGTGGACATTTCTAAATT11510.1754198799036791No Hit
GGTATCAACGCAGAGTACTTTTTTT10810.16475142500076204No Hit
CTTTAGGACGTGAAATATGGCGAGG10370.1580455390617856No Hit
GTCCTAAAGTGTGTATTTCTCATTT10170.15499740908952359No Hit
GTCCTACAGTGTGCATTTCTCATTT8590.13091718230865365No Hit
TATCAACGCAGAGTACTTTTTTTTT8200.12497332886274272No Hit
GTACATGGGAAGCAGTGGTATCAAC7330.11171396348340294No Hit
CTGTAGGACCTGGAATATGGCGAGA6680.10180754107355137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTTCG250.005905684519.0677811
TTAGACT400.00518837414.2855174
GCATTAT500.001458259413.3474461
TAGGACC2750.013.1599914
GGCGAGG3200.012.45124519
GTCTTAG1155.0258677E-912.4355091
TGTAGGA7750.012.4229092
GGACCTG4700.012.1569586
AGGACCT5100.011.7645445
AGTTGCC658.0920244E-411.6785648
CTGTAGG7700.011.6387751
AATCCCG1157.183553E-811.54898219
GCTATAA752.0029461E-411.4406681
TAGGAGT1001.8670053E-611.4284154
TGTAGAA3100.011.3774172
GTAGGAC7800.011.3560243
GGACGTG7050.011.3464946
TTAGGAC4450.011.3436763
AGGACGT7550.011.2265885
GGTATCA5550.011.1658161
Produced by FastQC (version 0.11.5)