FastQCFastQC Report
Thu 26 May 2016
SRR1926922_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926922_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636984
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14610.2293621189857202No Hit
GTCCTAAAGTGTGTATTTCTCATTT11810.18540497092548636No Hit
CTGTAGGACGTGGAATATGGCAAGA11500.18053828667596045No Hit
GTCCTACAGTGTGCATTTCTCATTT10250.16091455986335607No Hit
GTATCAACGCAGAGTACTTTTTTTT9940.15604787561383018No Hit
CTTTAGGACGTGAAATATGGCGAGG9510.14929731359029427No Hit
CTGTAGGACCTGGAATATGGCGAGA7220.113346646069603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTGC350.002194336116.25664712
GTCCTAT901.2551027E-1015.897421
AGGCGTG456.657431E-414.8060287
GTATTGG801.2224336E-714.3076791
GTCTATA500.001452926513.3538331
TAGGACC2950.013.2338444
GTCTAAA655.2401185E-513.20708751
GTCTTAC853.7592272E-612.343881
GCTGCTA550.003039357512.1044868
TTCTAGA959.975847E-712.0381162
GGACCTG3900.011.95871456
GTCCTAG1501.6370905E-1011.4461421
AATCCCG1001.965287E-611.37965319
CTGTAGG7450.011.2668971
GTTCTAA855.0969717E-511.2217091
AGGACCT4450.011.1223475
AAGGCGT600.005786323511.1045216
TAGCTGC600.005786323511.1045216
TTAGGAC3950.011.0984323
CTAAATT3850.011.08407719
Produced by FastQC (version 0.11.5)