##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926922_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 636984 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.1180484910139 31.0 28.0 31.0 16.0 34.0 2 28.29970140537282 31.0 28.0 33.0 16.0 34.0 3 27.930386006555896 31.0 28.0 33.0 16.0 34.0 4 31.63355751478844 35.0 32.0 37.0 19.0 37.0 5 31.698056780076108 35.0 32.0 37.0 18.0 37.0 6 31.218214272258017 35.0 32.0 37.0 13.0 37.0 7 31.138353553621442 35.0 32.0 37.0 11.0 37.0 8 30.73386929656004 35.0 32.0 37.0 10.0 37.0 9 32.93669385730254 37.0 33.0 39.0 17.0 39.0 10 33.121057985757886 37.0 33.0 39.0 17.0 39.0 11 33.2870495962222 38.0 34.0 39.0 17.0 39.0 12 33.168352737274404 38.0 33.0 39.0 17.0 39.0 13 33.27337735327732 38.0 34.0 39.0 17.0 39.0 14 34.2942177511523 39.0 34.0 41.0 17.0 41.0 15 33.87573628223001 39.0 33.0 41.0 10.0 41.0 16 33.97045137711465 39.0 33.0 41.0 11.0 41.0 17 34.20272251736307 39.0 34.0 41.0 16.0 41.0 18 34.25328736671565 39.0 34.0 41.0 13.0 41.0 19 33.64439138188715 39.0 33.0 41.0 10.0 41.0 20 33.91160531504716 39.0 34.0 41.0 10.0 41.0 21 33.886835462115215 39.0 34.0 41.0 8.0 41.0 22 33.920663627343856 39.0 34.0 41.0 2.0 41.0 23 33.917147055499036 39.0 34.0 41.0 2.0 41.0 24 32.66276232998003 39.0 32.0 41.0 2.0 41.0 25 33.233486241412656 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.1413887331122332 1101 2 -0.21935182741203363 1101 3 -0.9809649548701316 1101 4 -0.39027688629059654 1101 5 -0.45503620491470187 1101 6 -2.366896059648788 1101 7 -0.5491285179950012 1101 8 -0.22010515755297178 1101 9 -0.5055564108743482 1101 10 -8.971763861605648 1101 11 -4.245928637697897 1101 12 -1.5522619880539779 1101 13 -0.888488480129233 1101 14 -0.5595352914023266 1101 15 -2.0453202815981655 1101 16 -0.5392791433834816 1101 17 -0.45940175711162823 1101 18 -0.3257457056755868 1101 19 -0.9405132390122475 1101 20 -0.13139761567811092 1101 21 -0.32978149175725946 1101 22 -0.012936489175714883 1101 23 -0.2656493691668018 1101 24 -0.18573422980873744 1101 25 -0.24791315109195722 1102 1 -0.12299510167750682 1102 2 -0.4011388445806716 1102 3 -0.6518077554850876 1102 4 -0.5097050351806125 1102 5 -0.2852499178285015 1102 6 -0.9080684971647273 1102 7 -0.30131701030456526 1102 8 0.1272233725243197 1102 9 0.034358272440698556 1102 10 0.15014118555721012 1102 11 0.05659576273360045 1102 12 -0.012732236024604049 1102 13 -0.180751946289206 1102 14 -0.46381961171195485 1102 15 0.17308902788892766 1102 16 0.3593365079927935 1102 17 0.09133780235850253 1102 18 -0.2132100498064986 1102 19 0.15603665346439755 1102 20 0.2829358681957288 1102 21 0.06119286409709446 1102 22 -0.13010068050411405 1102 23 -0.680542114908576 1102 24 -4.4344361104730226 1102 25 -2.3323324430523975 1103 1 0.194864452286037 1103 2 -0.2991821861653463 1103 3 0.23263363176113927 1103 4 0.05292705782213858 1103 5 0.08896738400589399 1103 6 0.7351800569171338 1103 7 0.5026772733018667 1103 8 0.465949850979964 1103 9 0.495967492909287 1103 10 0.2002037086118591 1103 11 0.09067372747477265 1103 12 0.0063077160913209696 1103 13 0.0961915463251799 1103 14 -0.09406940233512273 1103 15 0.5147317022516944 1103 16 0.4770028088783249 1103 17 0.18597265147958097 1103 18 0.10396047457517454 1103 19 -1.4818852611562576 1103 20 -0.44240348467325674 1103 21 -0.30621070076100665 1103 22 -0.01252949818039184 1103 23 0.004717523965688031 1103 24 1.528546529229466 1103 25 0.6469305504911418 1104 1 -0.09416336709141149 1104 2 -0.22482804902202957 1104 3 -0.9151984416609196 1104 4 -0.29131035684886086 1104 5 -0.12685519425985348 1104 6 -1.3550418096589318 1104 7 -0.37319278074541984 1104 8 0.2090195938865378 1104 9 0.4795096690167142 1104 10 0.6132787713093961 1104 11 0.2535979137479245 1104 12 0.31393468698446014 1104 13 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0.41938167131045745 2215 2 -0.08312419351265277 2215 3 0.2550645297435743 2215 4 0.7225909283209297 2215 5 0.7982084896381743 2215 6 1.2913162374958809 2215 7 0.4310783498592414 2215 8 -0.5582746544519352 2215 9 -0.07741128633752226 2215 10 -0.03262343320689354 2215 11 0.022351624651754776 2215 12 -0.2812215061549139 2215 13 -0.02503117139283262 2215 14 -0.13405329326492677 2215 15 0.08065708975340868 2215 16 0.40682226942092825 2215 17 -0.07822395775759361 2215 18 -0.8146592531422527 2215 19 0.2763215242984316 2215 20 -3.397296123613991 2215 21 -1.8327081567066301 2215 22 -0.8378973546917976 2215 23 -0.7604063390679272 2215 24 -0.4923088231073294 2215 25 -0.4033513582372237 2216 1 -1.1668568607996335 2216 2 -1.693215936631919 2216 3 -0.886219873926148 2216 4 -1.433372374431361 2216 5 -1.3467456388021901 2216 6 -2.543546147825218 2216 7 -10.62121522812241 2216 8 -11.978458140690467 2216 9 -6.805851653309997 2216 10 -4.343632607518817 2216 11 -3.3556300267243877 2216 12 -2.8812215061549153 2216 13 -2.6057651163469586 2216 14 -2.990016596017224 2216 15 -3.7523704331823673 2216 16 -2.880333693881827 2216 17 -3.2736368017942894 2216 18 -2.9302555834174804 2216 19 -2.183311503224502 2216 20 -3.136745664898392 2216 21 -5.7886714594589215 2216 22 -4.541567079462439 2216 23 -4.422791660168844 2216 24 -4.384051942373386 2216 25 -3.771241266494105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39436.0 3 9.0 4 363.0 5 1807.0 6 2342.0 7 1982.0 8 1522.0 9 1376.0 10 1344.0 11 1231.0 12 1227.0 13 1121.0 14 1184.0 15 1287.0 16 1368.0 17 1302.0 18 1543.0 19 1985.0 20 2656.0 21 3309.0 22 4153.0 23 4816.0 24 5712.0 25 6395.0 26 7446.0 27 8119.0 28 8998.0 29 10628.0 30 12771.0 31 15359.0 32 19606.0 33 26062.0 34 35077.0 35 48801.0 36 71486.0 37 114824.0 38 168337.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.1424941431172 19.212935647451058 13.16850672695834 25.476063482473403 2 13.549434726291828 20.225040556345025 43.51931613828142 22.70620857908173 3 18.3436036092684 24.95567013550476 30.423491684220455 26.27723457100638 4 11.36563226101396 17.586833675551777 39.62245476668138 31.425079296752877 5 12.141368746182268 38.18579811598368 37.1258622774331 12.546970860400956 6 30.847562488419346 37.96915385074383 19.024461479999623 12.158822180837198 7 26.85058477644328 31.565330087287354 23.73403419371399 17.850050942555377 8 23.52565111957925 38.05215255251472 22.17837684628224 16.243819481623785 9 25.852043884676323 15.836514349510326 20.948699146268122 37.36274261954523 10 14.246317611233348 29.595585059686186 35.45615448521941 20.70194284386106 11 33.21138313507267 22.928469142936102 25.608756547626527 18.251391174364706 12 22.939905537131946 25.291270977791175 32.554988192141494 19.213835292935382 13 28.605591076385835 22.787938549661472 25.972251881068726 22.63421849288397 14 19.786673114939948 23.383993103697343 28.31817832092616 28.511155460436544 15 22.225257859028165 32.47006103200137 23.45296359277037 21.851717516200093 16 20.722803028637646 28.855436253835258 29.39245101703059 21.029309700496505 17 20.630187577136503 29.562162993948416 28.815214694641504 20.992434734273573 18 20.50496168823012 28.329198593141562 31.014947870870497 20.15089184775782 19 23.225967532732646 27.27354096723438 27.937301554898823 21.56318994513415 20 22.95933989149977 28.20016801048888 28.758842140799068 20.08164995721228 21 22.146470752640223 27.460518341032348 27.582833760940083 22.810177145387343 22 22.116322194988296 29.25515294354098 27.99304095504727 20.635483906423456 23 21.18775937682045 28.835244000672045 28.553864802948205 21.42313181955931 24 22.724396467124635 27.50963689892051 29.10916584887144 20.656800785083416 25 21.861923536495944 28.023847544038976 28.89559869300134 21.218630226463738 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 225.0 1 225.0 2 279.0 3 333.0 4 333.0 5 333.0 6 501.5 7 670.0 8 670.0 9 670.0 10 1010.0 11 1350.0 12 1350.0 13 1350.0 14 2365.5 15 3381.0 16 3381.0 17 3381.0 18 5888.5 19 8396.0 20 8396.0 21 8396.0 22 13392.5 23 18389.0 24 18389.0 25 18389.0 26 26694.0 27 34999.0 28 34999.0 29 34999.0 30 45442.5 31 55886.0 32 55886.0 33 55886.0 34 62651.0 35 69416.0 36 69416.0 37 69416.0 38 73639.0 39 77862.0 40 77862.0 41 77862.0 42 80363.0 43 82864.0 44 82864.0 45 82864.0 46 83003.5 47 83143.0 48 83143.0 49 83143.0 50 77728.5 51 72314.0 52 72314.0 53 72314.0 54 62544.5 55 52775.0 56 52775.0 57 52775.0 58 44003.5 59 35232.0 60 35232.0 61 35232.0 62 28184.5 63 21137.0 64 21137.0 65 21137.0 66 16043.5 67 10950.0 68 10950.0 69 10950.0 70 7906.0 71 4862.0 72 4862.0 73 4862.0 74 3368.5 75 1875.0 76 1875.0 77 1875.0 78 1262.0 79 649.0 80 649.0 81 649.0 82 421.0 83 193.0 84 193.0 85 193.0 86 129.5 87 66.0 88 66.0 89 66.0 90 40.0 91 14.0 92 14.0 93 14.0 94 8.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2208846690026751 2 0.13061552566469486 3 0.1324994034387049 4 0.07080240633987667 5 0.024019441618627783 6 0.022920512917121934 7 0.3069150873491328 8 0.3020484030996069 9 0.020879645328611078 10 0.03720658603669794 11 0.01758285922409354 12 0.01883877774010022 13 0.01758285922409354 14 0.01899576755460106 15 0.016483930522587696 16 0.020408675885108574 17 0.019780716627105233 18 0.016326940708086858 19 0.024804390691131956 20 0.018681787925599387 21 0.01664092033708853 22 0.01805382866759605 23 0.01899576755460106 24 0.017111889780591034 25 0.016483930522587696 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 636984.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.44031759915869 #Duplication Level Percentage of deduplicated Percentage of total 1 94.3507638587556 81.55709993674056 2 4.307157157126177 7.446240652229523 3 0.6466385305777564 1.6768691986498367 4 0.23522824689957783 0.8133281748117129 5 0.11470364236048354 0.4957509637710255 6 0.06851217336516614 0.35533284150561495 7 0.04410728842557011 0.2668853613960784 8 0.03422050036364714 0.23664247358686274 9 0.026358346997225265 0.20505814972460842 >10 0.14285955663146258 2.4001712793096464 >50 0.014543637710603761 0.8856648483317027 >100 0.013270919969291094 2.310156497582364 >500 9.089671207569818E-4 0.5938426911238123 >1k 7.271736966055854E-4 0.7569569312366773 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1461 0.2293621189857202 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1181 0.18540497092548636 No Hit CTGTAGGACGTGGAATATGGCAAGA 1150 0.18053828667596045 No Hit GTCCTACAGTGTGCATTTCTCATTT 1025 0.16091455986335607 No Hit GTATCAACGCAGAGTACTTTTTTTT 994 0.15604787561383018 No Hit CTTTAGGACGTGAAATATGGCGAGG 951 0.14929731359029427 No Hit CTGTAGGACCTGGAATATGGCGAGA 722 0.113346646069603 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.569898145008352E-4 0.0 9 0.0 0.0 0.0 1.569898145008352E-4 0.0 10 0.0 0.0 0.0 1.569898145008352E-4 0.0 11 0.0 0.0 0.0 1.569898145008352E-4 0.0 12 0.0 0.0 0.0 1.569898145008352E-4 1.569898145008352E-4 13 0.0 0.0 0.0 1.569898145008352E-4 1.569898145008352E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGTGC 35 0.0021943361 16.256647 12 GTCCTAT 90 1.2551027E-10 15.89742 1 AGGCGTG 45 6.657431E-4 14.806028 7 GTATTGG 80 1.2224336E-7 14.307679 1 GTCTATA 50 0.0014529265 13.353833 1 TAGGACC 295 0.0 13.233844 4 GTCTAAA 65 5.2401185E-5 13.2070875 1 GTCTTAC 85 3.7592272E-6 12.34388 1 GCTGCTA 55 0.0030393575 12.104486 8 TTCTAGA 95 9.975847E-7 12.038116 2 GGACCTG 390 0.0 11.9587145 6 GTCCTAG 150 1.6370905E-10 11.446142 1 AATCCCG 100 1.965287E-6 11.379653 19 CTGTAGG 745 0.0 11.266897 1 GTTCTAA 85 5.0969717E-5 11.221709 1 AGGACCT 445 0.0 11.122347 5 AAGGCGT 60 0.0057863235 11.104521 6 TAGCTGC 60 0.0057863235 11.104521 6 TTAGGAC 395 0.0 11.098432 3 CTAAATT 385 0.0 11.084077 19 >>END_MODULE