FastQCFastQC Report
Thu 26 May 2016
SRR1926922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926922_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636984
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15840.24867186616932294No Hit
GTCCTACAGTGGACATTTCTAAATT14450.22685028195370682No Hit
CTGTAGGACGTGGAATATGGCAAGA14070.2208846690026751No Hit
CTTTAGGACGTGAAATATGGCGAGG12750.20016201348856485No Hit
GTCCTAAAGTGTGTATTTCTCATTT12330.19356844127952977No Hit
GTCCTACAGTGTGCATTTCTCATTT10570.16593823392738277No Hit
GGTATCAACGCAGAGTACTTTTTTT9890.155262926541326No Hit
CTGTAGGACCTGGAATATGGCGAGA8460.13281338306770657No Hit
TATCAACGCAGAGTACTTTTTTTTT7820.12276603493965313No Hit
CTGAAGGACCTGGAATATGGCGAGA6430.10094445072403703No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG551.9770241E-413.80185218
TAGGACC3800.012.7780694
TTAGGGA550.003014942412.1175274
CAAAAGT550.003014942412.1175274
GACGAAA550.003092290412.07662119
TGTACTG802.7964606E-511.9011425
CTATACT1052.6336784E-711.7877984
AATGCCG658.091883E-411.67849118
GTGTAGA1001.8520568E-611.4359061
TATAGGA1251.7398634E-811.4359062
GTATAAA1354.474714E-911.2947231
CTACACT1354.5420165E-911.2840464
GATATAG600.005733364711.1182421
TGACGAA600.00592108711.07023618
TAAAGAG1053.3652377E-610.8810445
TTGTACT700.001462224410.8810444
TGTAGGA8700.010.8443942
GTCCTAC7800.010.7517071
GGACGTG6450.010.6279976
TGAACTG909.284496E-510.5787945