##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926922_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 636984 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.68478172136192 31.0 30.0 33.0 25.0 34.0 2 30.022805910352535 31.0 30.0 34.0 26.0 34.0 3 30.07616046870879 31.0 30.0 34.0 26.0 34.0 4 33.63882923275938 35.0 35.0 37.0 28.0 37.0 5 33.548671552189695 37.0 35.0 37.0 28.0 37.0 6 33.449485387388066 37.0 35.0 37.0 28.0 37.0 7 33.44542092109064 37.0 35.0 37.0 28.0 37.0 8 33.471399595594235 37.0 35.0 37.0 28.0 37.0 9 34.98567938912123 39.0 35.0 39.0 28.0 39.0 10 34.90674020069578 39.0 35.0 39.0 28.0 39.0 11 34.91423489444005 39.0 35.0 39.0 28.0 39.0 12 34.812202504301524 39.0 35.0 39.0 27.0 39.0 13 34.82109126759856 39.0 35.0 39.0 27.0 39.0 14 35.902071323612525 40.0 36.0 41.0 27.0 41.0 15 35.875014129083304 40.0 36.0 41.0 27.0 41.0 16 35.864811361038896 40.0 36.0 41.0 27.0 41.0 17 35.84802914986876 40.0 36.0 41.0 27.0 41.0 18 35.82529545483089 40.0 36.0 41.0 27.0 41.0 19 35.88914007259209 40.0 36.0 41.0 27.0 41.0 20 35.84780465443402 40.0 36.0 41.0 27.0 41.0 21 35.78876863469098 40.0 36.0 41.0 27.0 41.0 22 35.69896418120393 40.0 36.0 41.0 27.0 41.0 23 35.618637202818284 40.0 36.0 41.0 27.0 41.0 24 35.55502963967698 40.0 36.0 41.0 27.0 41.0 25 35.43712714919056 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03007204732338309 1101 2 0.076253463071847 1101 3 0.34025004791263314 1101 4 0.2459612986490356 1101 5 0.19641668447477656 1101 6 0.3210210275511258 1101 7 0.3162261373878863 1101 8 0.36939605765569894 1101 9 0.31259612379875534 1101 10 0.3321780936077232 1101 11 0.47955588056723997 1101 12 0.307782263055735 1101 13 0.4324103706643285 1101 14 0.11864329289677045 1101 15 0.2154319989309812 1101 16 0.2555407557513334 1101 17 0.2424108049320779 1101 18 0.27985817975268645 1101 19 0.15544422114251688 1101 20 0.1530269261460191 1101 21 0.1292874986096848 1101 22 0.03599999667149234 1101 23 0.056410493620710156 1101 24 0.05652735145758214 1101 25 -0.12859706218308276 1102 1 -1.2976577910597662 1102 2 -1.1833715740757782 1102 3 -1.0700293724206311 1102 4 -1.3003474560222017 1102 5 -1.1000283756517248 1102 6 -1.0425647068252246 1102 7 -1.0711914065867134 1102 8 -1.1890514875591052 1102 9 -1.1998655878233606 1102 10 -0.8738783146206828 1102 11 -0.9329254871205279 1102 12 -0.8335881391214102 1102 13 -0.8353134335331802 1102 14 -1.1053867153634584 1102 15 -0.926061253656485 1102 16 -1.0602503189908603 1102 17 -0.9737474170363853 1102 18 -1.196325220699876 1102 19 -1.0858012246172706 1102 20 -1.1024469371366834 1102 21 -1.291126495573053 1102 22 -1.0902878851300315 1102 23 -0.8934899390467308 1102 24 -1.027900130530206 1102 25 -0.9497221847409918 1103 1 -0.6161344556925954 1103 2 -0.5361408002050787 1103 3 -0.3133890528222629 1103 4 -0.29938351059705326 1103 5 -0.4602748487250494 1103 6 -0.4984946531644354 1103 7 -0.6564037181765627 1103 8 -0.5008478131986607 1103 9 -0.7052421065303989 1103 10 -0.48383885201735666 1103 11 -0.5326630990878343 1103 12 -0.4056798890941451 1103 13 -0.5037521667395382 1103 14 -0.6528636375123895 1103 15 -0.5469569457136174 1103 16 -0.48792773250836774 1103 17 -0.46454411619028946 1103 18 -0.5470018384694129 1103 19 -0.5499960459915698 1103 20 -0.4813702878156221 1103 21 -0.18215542321566858 1103 22 -0.3778080978244205 1103 23 -0.39538651037057093 1103 24 -0.45260450900000393 1103 25 -0.39191329881134607 1104 1 -0.06715885073401395 1104 2 -0.20130913359056635 1104 3 0.25048420204042543 1104 4 0.13384417198577125 1104 5 0.05716870114016359 1104 6 0.13969474033468998 1104 7 0.013778182911089232 1104 8 -0.11205603160513533 1104 9 -0.13692749677506555 1104 10 -0.08602577784873944 1104 11 0.08952445260131014 1104 12 0.06613863003698128 1104 13 0.29722953586179557 1104 14 0.04729685029902697 1104 15 0.0324621688764708 1104 16 -0.06763071829692535 1104 17 -0.058407133808188405 1104 18 0.04692600013081005 1104 19 0.042161509229195815 1104 20 0.16230877232484175 1104 21 0.034766876543372405 1104 22 0.027662793084104464 1104 23 -0.0572780313390453 1104 24 0.09502773697997924 1104 25 0.06790663297015698 1105 1 0.07263096197655372 1105 2 -0.009731460399979852 1105 3 0.32718987748424766 1105 4 0.04683123523330579 1105 5 0.23687173849270238 1105 6 -0.060189587855049353 1105 7 0.09150512433772207 1105 8 -0.23852746088300592 1105 9 -0.10961561934418285 1105 10 0.25375478971714216 1105 11 0.020034290080268136 1105 12 0.3039441766937898 1105 13 0.3856915952930251 1105 14 0.188360557946055 1105 15 0.07740324720434444 1105 16 -0.05946704053536678 1105 17 0.21636609139278562 1105 18 0.340372267564895 1105 19 -0.09888822319911128 1105 20 0.042867953076019205 1105 21 0.13611731267769756 1105 22 -0.008844778259387454 1105 23 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15 0.2936138910594934 2211 16 0.35908830940987 2211 17 0.509561524053936 2211 18 0.5193474844338795 2211 19 0.5586726169838343 2211 20 0.2992433755099526 2211 21 0.2830686946273815 2211 22 0.22291388399658274 2211 23 0.40227182372322545 2211 24 0.25834597306398877 2211 25 0.2752927673639576 2212 1 -0.20674927501287144 2212 2 -0.19460945933404972 2212 3 -0.2313562080226923 2212 4 -0.08549654270030516 2212 5 -0.21307857146894804 2212 6 -0.21687049365635858 2212 7 -0.1916168927572528 2212 8 -0.20920232373449466 2212 9 -0.2670989791922267 2212 10 -0.320096027964496 2212 11 -0.5044029902717355 2212 12 -0.22579170472334198 2212 13 -0.5056164548939108 2212 14 -0.5374756572759267 2212 15 -0.34552008988245575 2212 16 -0.3795219800858334 2212 17 -0.18027662520970011 2212 18 -0.2429166519572803 2212 19 -0.33894821189863933 2212 20 -0.4099295159378329 2212 21 -0.4558954143837326 2212 22 -0.29360476335237706 2212 23 -0.5002282437627841 2212 24 -0.5665239717517352 2212 25 -0.6173630192722683 2213 1 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0.041329282858882266 2214 13 0.010668650339660246 2214 14 0.1380797117168484 2214 15 0.03743265538878404 2214 16 -0.015090051570197716 2214 17 -0.1081315831965739 2214 18 0.020451990409178222 2214 19 0.0997020388660701 2214 20 -0.005718347965633086 2214 21 -0.21127024738976985 2214 22 -0.020935561054244545 2214 23 -0.15805670172410657 2214 24 -0.2490638860464145 2214 25 -0.26593654406767797 2215 1 0.6641142526350059 2215 2 0.31936084016975386 2215 3 0.37352723894835904 2215 4 0.4755786723784041 2215 5 0.5384599999086248 2215 6 0.6083909920824055 2215 7 0.5768281149177525 2215 8 0.3762223005470915 2215 9 0.4276891548883768 2215 10 0.5777960221550913 2215 11 0.32179734528676107 2215 12 0.5424980179054373 2215 13 0.4225900920664287 2215 14 0.44612659881945405 2215 15 0.5127153722244202 2215 16 0.5750901642757142 2215 17 0.5418916538036171 2215 18 0.5923455477357251 2215 19 0.6067609231458064 2215 20 0.706418251170291 2215 21 0.4891979351325588 2215 22 0.6514279000539034 2215 23 0.6898442225921215 2215 24 0.9222633769792097 2215 25 0.6941405683477626 2216 1 -0.5395554721356355 2216 2 -0.42284099469263126 2216 3 -2.4292250546296223 2216 4 -1.8060727037683826 2216 5 -0.9652097248620137 2216 6 -0.6136273565414498 2216 7 -0.4607865639813298 2216 8 -0.5375391673428211 2216 9 -0.5346961662125409 2216 10 -0.7185342530742673 2216 11 -0.5488448565481079 2216 12 -0.3125478536541948 2216 13 -0.27007045839228283 2216 14 -0.5391945020979776 2216 15 -0.642330499335209 2216 16 -0.4909648815958505 2216 17 -0.7415945847284888 2216 18 -0.5975627091450093 2216 19 -0.7638812786890554 2216 20 -0.577067987361815 2216 21 -0.5575910556931234 2216 22 -0.3201317329736213 2216 23 -0.5358438508023724 2216 24 -0.6832412101767531 2216 25 -0.7379695233953569 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18130.0 3 17.0 4 311.0 5 2130.0 6 2664.0 7 1982.0 8 1564.0 9 1349.0 10 1073.0 11 1022.0 12 994.0 13 879.0 14 870.0 15 869.0 16 899.0 17 999.0 18 1086.0 19 1247.0 20 1656.0 21 2085.0 22 2672.0 23 3206.0 24 3744.0 25 4586.0 26 5137.0 27 5755.0 28 6655.0 29 8138.0 30 10153.0 31 13021.0 32 16859.0 33 22290.0 34 30055.0 35 41958.0 36 61245.0 37 105865.0 38 253819.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.7865744218722 20.263469741213747 10.922269825453654 28.0276860114604 2 13.395976445184136 21.257482677277963 41.37258507346343 23.973955804074475 3 18.004544119886884 25.578495981107512 29.77152215454718 26.645437744458416 4 11.713951998794318 18.147080617409543 38.75089484194266 31.388072541853486 5 12.48178803788088 39.18036750483559 35.54975005651988 12.788094400763647 6 30.54255091239036 38.781631212823875 18.27096355479383 12.404854319991934 7 27.312326388071046 32.30135811832541 22.235831146745557 18.150484346857986 8 24.178389750675127 38.96125102053633 20.18196947811342 16.678389750675123 9 26.09515495690975 15.96294034249253 20.452679226086833 37.48922547451089 10 14.535832089136317 30.048339212851104 34.53341434351142 20.882414354501165 11 33.22845870404474 23.220781504385737 25.171795004639293 18.378964786930236 12 23.178326580144212 25.496912790451237 31.96301942124044 19.36174120816411 13 28.585669879745048 22.98470909604697 25.792646550912117 22.636974473295865 14 20.09924896072743 23.65616718787285 27.730209863984022 28.5143739874157 15 21.925640776058856 32.571173790587196 23.45116349169966 22.05202194165429 16 20.863573638774156 29.050474032977437 29.070411833319987 21.01554049492842 17 20.66378433367243 29.858206799542845 28.25895155922284 21.219057307561886 18 20.42440693079721 28.358213641494356 30.89470205641281 20.322677371295622 19 22.941078582821547 27.49409718297477 27.90666641548296 21.65815781872072 20 22.954095889980845 28.306383245941785 28.556312600081636 20.18320826399573 21 22.036945445410083 27.38155239956978 27.533228133807512 23.04827402121262 22 22.37230448488502 29.327738216344525 27.56034688469412 20.739610414076335 23 21.193938937241754 29.090369616819263 28.275121510116424 21.440569935822566 24 22.439531353270027 27.60670221968247 29.049911850080772 20.903854576966733 25 21.728980032718834 28.375425077950585 28.57811229279903 21.317482596531555 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 241.0 1 241.0 2 243.0 3 245.0 4 245.0 5 245.0 6 430.0 7 615.0 8 615.0 9 615.0 10 1010.5 11 1406.0 12 1406.0 13 1406.0 14 2323.0 15 3240.0 16 3240.0 17 3240.0 18 5689.5 19 8139.0 20 8139.0 21 8139.0 22 12833.5 23 17528.0 24 17528.0 25 17528.0 26 25430.5 27 33333.0 28 33333.0 29 33333.0 30 43783.0 31 54233.0 32 54233.0 33 54233.0 34 60997.5 35 67762.0 36 67762.0 37 67762.0 38 72250.0 39 76738.0 40 76738.0 41 76738.0 42 79469.5 43 82201.0 44 82201.0 45 82201.0 46 83138.5 47 84076.0 48 84076.0 49 84076.0 50 79462.5 51 74849.0 52 74849.0 53 74849.0 54 64551.5 55 54254.0 56 54254.0 57 54254.0 58 45424.0 59 36594.0 60 36594.0 61 36594.0 62 29377.5 63 22161.0 64 22161.0 65 22161.0 66 16761.0 67 11361.0 68 11361.0 69 11361.0 70 8226.5 71 5092.0 72 5092.0 73 5092.0 74 3480.5 75 1869.0 76 1869.0 77 1869.0 78 1306.5 79 744.0 80 744.0 81 744.0 82 485.5 83 227.0 84 227.0 85 227.0 86 145.0 87 63.0 88 63.0 89 63.0 90 37.5 91 12.0 92 12.0 93 12.0 94 6.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11083480903758963 2 0.10691006367506876 3 0.018367808296597714 4 0.0 5 0.006279592580033407 6 0.34035391783781066 7 0.023077502731622773 8 0.010047348128053453 9 0.010204337942554287 10 0.00423872499152255 11 0.005180663878527561 12 1.569898145008352E-4 13 6.279592580033408E-4 14 0.0 15 0.003453775919018374 16 4.7096944350250556E-4 17 0.0 18 1.569898145008352E-4 19 0.0 20 6.279592580033408E-4 21 0.015541991635582683 22 0.0 23 0.0 24 1.569898145008352E-4 25 0.006593572209035078 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 636984.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.95515717287782 #Duplication Level Percentage of deduplicated Percentage of total 1 92.86932820837733 77.96859046273893 2 5.488024391931897 9.214959007864591 3 0.8399979218178579 2.1156647265332698 4 0.26624605296197584 0.8941091689232412 5 0.13706248295443305 0.5753551149472156 6 0.08090744043282325 0.4075558126795755 7 0.05878076013057652 0.34544635688526476 8 0.038564859742182704 0.25901750888039493 9 0.027185080896336117 0.20540949654683666 >10 0.15818583826929197 2.5035236405121086 >50 0.018700125522528005 1.0760551045207989 >100 0.01477285846297537 2.501395594920736 >500 0.0011219892499157152 0.6768038917679183 >1k 0.0011219892499157152 1.2561141122790802 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1584 0.24867186616932294 No Hit GTCCTACAGTGGACATTTCTAAATT 1445 0.22685028195370682 No Hit CTGTAGGACGTGGAATATGGCAAGA 1407 0.2208846690026751 No Hit CTTTAGGACGTGAAATATGGCGAGG 1275 0.20016201348856485 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1233 0.19356844127952977 No Hit GTCCTACAGTGTGCATTTCTCATTT 1057 0.16593823392738277 No Hit GGTATCAACGCAGAGTACTTTTTTT 989 0.155262926541326 No Hit CTGTAGGACCTGGAATATGGCGAGA 846 0.13281338306770657 No Hit TATCAACGCAGAGTACTTTTTTTTT 782 0.12276603493965313 No Hit CTGAAGGACCTGGAATATGGCGAGA 643 0.10094445072403703 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 1.569898145008352E-4 13 0.0 0.0 0.0 0.0 1.569898145008352E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 55 1.9770241E-4 13.801852 18 TAGGACC 380 0.0 12.778069 4 TTAGGGA 55 0.0030149424 12.117527 4 CAAAAGT 55 0.0030149424 12.117527 4 GACGAAA 55 0.0030922904 12.076621 19 TGTACTG 80 2.7964606E-5 11.901142 5 CTATACT 105 2.6336784E-7 11.787798 4 AATGCCG 65 8.091883E-4 11.678491 18 GTGTAGA 100 1.8520568E-6 11.435906 1 TATAGGA 125 1.7398634E-8 11.435906 2 GTATAAA 135 4.474714E-9 11.294723 1 CTACACT 135 4.5420165E-9 11.284046 4 GATATAG 60 0.0057333647 11.118242 1 TGACGAA 60 0.005921087 11.070236 18 TAAAGAG 105 3.3652377E-6 10.881044 5 TTGTACT 70 0.0014622244 10.881044 4 TGTAGGA 870 0.0 10.844394 2 GTCCTAC 780 0.0 10.751707 1 GGACGTG 645 0.0 10.627997 6 TGAACTG 90 9.284496E-5 10.578794 5 >>END_MODULE