FastQCFastQC Report
Thu 26 May 2016
SRR1926921_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926921_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533234
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8910.16709362118694607No Hit
GTCCTACAGTGGACATTTCTAAATT7150.13408747379199376No Hit
GTCCTACAGTGTGCATTTCTCATTT5830.10933286324577952No Hit
GTCCTAAAGTGTGTATTTCTCATTT5520.1035192804659868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGG402.7446944E-416.6346238
CGAAATC350.002192068416.25846513
CCTTATA551.0888272E-515.5999072
ACGAAAT456.8382965E-414.75305112
GTATTAG653.2169064E-614.6776491
TTAGGAC2150.014.6324723
GTCTTAC500.001450065513.3566611
GGCGAGG2000.012.80474119
CTTTAGG2350.012.5853031
TAGGACC1900.012.5260734
AGGACGT2700.012.3335355
TAGGACG2800.012.2397634
GTTACAA1650.012.07067815
GTCTATA802.7363343E-511.92559051
GTCCTAC4050.011.7783591
TTTAGGA2950.011.6338292
GACGTGA1800.011.6276687
GGACGTG2700.011.2763756
TGTCCAC1354.818503E-911.24041810
TTACAAT1701.2732926E-1111.15776916
Produced by FastQC (version 0.11.5)