FastQCFastQC Report
Thu 26 May 2016
SRR1926921_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926921_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533234
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15110.2833652767828008No Hit
GGTATCAACGCAGAGTACTTTTTTT9470.17759557717624908No Hit
TATCAACGCAGAGTACTTTTTTTTT7820.14665231399348128No Hit
GTCCTACAGTGGACATTTCTAAATT7200.1350251484338958No Hit
CTGTAGGACGTGGAATATGGCAAGA6580.12339798287431035No Hit
GTCCTACAGTGTGCATTTCTCATTT6340.11889714459318047No Hit
CTTTAGGACGTGAAATATGGCGAGG5830.10933286324577952No Hit
GTCCTAAAGTGTGTATTTCTCATTT5830.10933286324577952No Hit
GTACATGGGAAGCAGTGGTATCAAC5570.10445695510788884No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG456.544713E-414.8386561
GTCTTAC400.005131150614.3087031
TAATACC400.005179516514.2884924
TTAGTCT400.005179516514.2884924
TAAGACC400.005179516514.2884924
TAGGACC1600.013.6931384
GTAATAG500.001451598113.354791
TCTAATC500.001466109413.3371813
TCAGGAC1259.640644E-1112.956123
AGGACCT2800.012.58748155
GGACCTG2600.012.4566356
TAGACAG853.8127764E-612.3273275
CAGGACC853.8127764E-612.3273274
ATAATAC701.06029634E-412.2484333
TTGGACC701.0612294E-412.2472794
TAGCACT701.0612294E-412.2472794
TTGGGCT550.00300259112.12356954
TGTAGGA4650.012.1045622
GTCTATA959.85312E-712.0494361
GGCGAGG1353.765308E-1011.94578119
Produced by FastQC (version 0.11.5)