##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926921_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 533234 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.640940000075013 31.0 30.0 33.0 25.0 34.0 2 29.969630593698078 31.0 30.0 34.0 26.0 34.0 3 30.0175889009328 31.0 30.0 34.0 26.0 34.0 4 33.58361619851697 35.0 35.0 37.0 28.0 37.0 5 33.55492710517334 37.0 35.0 37.0 28.0 37.0 6 33.45664004920916 37.0 35.0 37.0 28.0 37.0 7 33.449314934906624 37.0 35.0 37.0 28.0 37.0 8 33.44641564491386 37.0 35.0 37.0 28.0 37.0 9 34.967535828548066 39.0 35.0 39.0 28.0 39.0 10 34.8589306008244 39.0 35.0 39.0 28.0 39.0 11 34.858431757914914 39.0 35.0 39.0 27.0 39.0 12 34.772345724391165 39.0 35.0 39.0 27.0 39.0 13 34.77495433524494 39.0 35.0 39.0 27.0 39.0 14 35.85895873106366 40.0 36.0 41.0 27.0 41.0 15 35.82582693526669 40.0 36.0 41.0 27.0 41.0 16 35.80842744461156 40.0 36.0 41.0 27.0 41.0 17 35.78748166846075 40.0 36.0 41.0 27.0 41.0 18 35.75986902560602 40.0 36.0 41.0 27.0 41.0 19 35.82324645465218 40.0 36.0 41.0 27.0 41.0 20 35.784012272285715 40.0 36.0 41.0 27.0 41.0 21 35.737689269626465 40.0 36.0 41.0 27.0 41.0 22 35.64292411961728 40.0 36.0 41.0 27.0 41.0 23 35.564558899094955 40.0 36.0 41.0 27.0 41.0 24 35.47878417355233 40.0 36.0 41.0 27.0 41.0 25 35.359541964690926 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05827177740864897 1101 2 0.1075653786489994 1101 3 0.3668651243220822 1101 4 0.47905888972373134 1101 5 0.023771589314847574 1101 6 0.12588222522609982 1101 7 0.31435702293284606 1101 8 0.31881853586680364 1101 9 0.3939888103511464 1101 10 0.5033943546990898 1101 11 0.38831502205285773 1101 12 0.34911910341492103 1101 13 0.2665337317442251 1101 14 0.35251332934788593 1101 15 0.30230516137289953 1101 16 0.462712020997202 1101 17 0.4220383437823543 1101 18 0.5983734848799926 1101 19 0.33205247893366874 1101 20 0.5698783644058807 1101 21 0.6297604305943452 1101 22 0.5764914378059842 1101 23 0.5756195541861757 1101 24 0.7208098768149256 1101 25 0.4867346330789033 1102 1 -0.8447391927189969 1102 2 -0.656631466549257 1102 3 -0.3506837570666761 1102 4 -0.556728125556603 1102 5 -0.6274818343998021 1102 6 -0.658280157347015 1102 7 -0.48226127263875185 1102 8 -0.6120900717197202 1102 9 -0.7676342558731122 1102 10 -0.5894268811135603 1102 11 -0.6587554174642918 1102 12 -0.6023341655502037 1102 13 -0.6413570701526368 1102 14 -0.5019081874823144 1102 15 -0.4900361610235322 1102 16 -0.6735672496863074 1102 17 -0.7128073576459926 1102 18 -0.7066207476145578 1102 19 -0.5927800735316424 1102 20 -0.7601795954636756 1102 21 -0.68476561337161 1102 22 -0.7341848556105148 1102 23 -0.6918550379348929 1102 24 -0.6140234826264575 1102 25 -0.6736494875307955 1103 1 -0.48098760843509325 1103 2 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2214 12 0.012355041466250327 2214 13 -0.035957053752333934 2214 14 0.1503837552201972 2214 15 0.011403181583169442 2214 16 0.17761436355708327 2214 17 0.23432179763756267 2214 18 0.1715134730616228 2214 19 0.15681891022486383 2214 20 0.16742456445944498 2214 21 0.034687019237090055 2214 22 0.28344259562375385 2214 23 0.04885037661160396 2214 24 0.012777493911031002 2214 25 -0.14971583108266628 2215 1 0.35812539398611065 2215 2 0.22221774990456566 2215 3 0.2347884494505763 2215 4 0.4244690679770713 2215 5 0.4009108349513326 2215 6 0.1671544147848394 2215 7 0.07585407329979432 2215 8 0.1594306538067798 2215 9 -0.0598848237696572 2215 10 0.10866075694033128 2215 11 0.045436299230502186 2215 12 0.19396684623355753 2215 13 0.07073988154846234 2215 14 0.036876377240631086 2215 15 -0.13956163112965925 2215 16 -0.1765286557391761 2215 17 -0.009719065018508388 2215 18 -0.22008698096789203 2215 19 -0.25634794562076735 2215 20 -0.2502825865053637 2215 21 -0.04703829290819783 2215 22 -0.057079538314951606 2215 23 -0.07033237026693939 2215 24 0.23638702853078541 2215 25 0.13405261386625256 2216 1 -0.1740302238426743 2216 2 -0.03178843139790999 2216 3 -1.9013705971923045 2216 4 -1.4252581735314962 2216 5 -0.5625898584135953 2216 6 -0.3088976255954776 2216 7 -0.30375601706046496 2216 8 -0.4069659194762565 2216 9 -0.1975393890993118 2216 10 -0.23079183865620934 2216 11 -0.2277350654532384 2216 12 -0.28258981701652885 2216 13 -0.16955366478378409 2216 14 -0.2658462269803792 2216 15 -0.1861258702763351 2216 16 -0.28189128952976716 2216 17 -0.4210561714454357 2216 18 -0.40233579018899235 2216 19 -0.7216390671418225 2216 20 -0.2093727449176228 2216 21 -0.043693364171438986 2216 22 -0.2428856110907489 2216 23 -0.4054779769022758 2216 24 -0.5762523981127785 2216 25 -0.5301726392971418 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15372.0 3 11.0 4 233.0 5 1774.0 6 2257.0 7 1710.0 8 1282.0 9 1155.0 10 920.0 11 864.0 12 883.0 13 762.0 14 735.0 15 691.0 16 760.0 17 858.0 18 918.0 19 1059.0 20 1398.0 21 1793.0 22 2206.0 23 2713.0 24 3211.0 25 3769.0 26 4286.0 27 4933.0 28 5645.0 29 6729.0 30 8553.0 31 11138.0 32 14613.0 33 18772.0 34 25362.0 35 35325.0 36 52183.0 37 89789.0 38 208572.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.261499755923545 19.808306109421352 10.779730389395818 28.150463745259284 2 13.836018669059753 21.415912256921107 40.26426557225625 24.483803501762896 3 18.158457922802356 25.50121822898203 29.496334059206493 26.843989789009115 4 11.882963201896352 18.111748313123318 38.26912762502016 31.736160859960165 5 12.598977498208935 38.862757454023054 35.5625072674146 12.975757780353412 6 30.718190457372437 38.69628626000885 18.048004817417976 12.537518465200742 7 27.354937217930875 32.434901353181495 21.939022422971448 18.271139005916183 8 24.644071547667497 38.269037084828284 19.96837858671413 17.118512780790084 9 25.894298955518085 16.127670987259535 20.063655442877963 37.91437461434442 10 14.315748016728868 30.431912379737813 34.048498715327916 21.2038408882054 11 33.5926437850824 23.521733504123148 24.249778229549765 18.63584448124469 12 22.63521813247917 25.798950173564556 31.834608265460933 19.731223428495344 13 28.47058779403298 22.608963094793815 25.761442976871184 23.159006134302018 14 20.418278688678306 23.114473410310314 27.710775589657803 28.756472311353576 15 22.53443205857201 31.391310218594814 23.495191514260423 22.57906620857276 16 21.526545768242595 29.189843032087463 27.802824297207586 21.480786902462352 17 21.11440964831509 29.321141039658084 27.76328546807868 21.801163843948142 18 20.845898220661926 28.574429141536896 29.684077474720194 20.895595163080984 19 22.686415455965616 27.84928108080738 27.743135714165156 21.721167749061852 20 22.690771336946533 28.225718733004516 28.404065787746376 20.67944414230257 21 22.45544079059657 27.298388684971126 27.60354635601978 22.642624168412524 22 22.351350439019267 28.50605925353597 27.709035807919225 21.43355449952554 23 21.801726449788365 28.24693895539098 27.908250239576322 22.04308435524433 24 22.19761342602577 27.638949577389248 28.672081435320017 21.491355561264964 25 22.131930000918967 27.852972672901892 28.133351587657323 21.88174573852181 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 264.0 1 264.0 2 264.5 3 265.0 4 265.0 5 265.0 6 425.5 7 586.0 8 586.0 9 586.0 10 942.0 11 1298.0 12 1298.0 13 1298.0 14 2096.5 15 2895.0 16 2895.0 17 2895.0 18 4910.0 19 6925.0 20 6925.0 21 6925.0 22 10670.0 23 14415.0 24 14415.0 25 14415.0 26 20341.0 27 26267.0 28 26267.0 29 26267.0 30 34246.0 31 42225.0 32 42225.0 33 42225.0 34 47543.0 35 52861.0 36 52861.0 37 52861.0 38 57129.0 39 61397.0 40 61397.0 41 61397.0 42 64215.5 43 67034.0 44 67034.0 45 67034.0 46 69053.5 47 71073.0 48 71073.0 49 71073.0 50 68100.5 51 65128.0 52 65128.0 53 65128.0 54 56790.0 55 48452.0 56 48452.0 57 48452.0 58 40969.5 59 33487.0 60 33487.0 61 33487.0 62 27019.0 63 20551.0 64 20551.0 65 20551.0 66 15735.5 67 10920.0 68 10920.0 69 10920.0 70 7726.0 71 4532.0 72 4532.0 73 4532.0 74 3160.0 75 1788.0 76 1788.0 77 1788.0 78 1199.0 79 610.0 80 610.0 81 610.0 82 401.0 83 192.0 84 192.0 85 192.0 86 124.0 87 56.0 88 56.0 89 56.0 90 33.5 91 11.0 92 11.0 93 11.0 94 6.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11514644602557227 2 0.11027053788768158 3 0.015565399055574101 4 0.0 5 0.006001117708173147 6 0.3430013840077714 7 0.022504191405649302 8 0.009564281347400954 9 0.009564281347400954 10 0.004500838281129861 11 0.004688373209510271 12 5.626047851412326E-4 13 5.626047851412326E-4 14 1.8753492838041085E-4 15 0.0018753492838041086 16 7.501397135216434E-4 17 1.8753492838041085E-4 18 3.750698567608217E-4 19 5.626047851412326E-4 20 7.501397135216434E-4 21 0.012564840201487525 22 0.0 23 1.8753492838041085E-4 24 1.8753492838041085E-4 25 0.005063443066271093 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 533234.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.24677982244398 #Duplication Level Percentage of deduplicated Percentage of total 1 93.61155151025548 80.73694871942378 2 4.904240663369788 8.459499293798615 3 0.736161123171732 1.904745789121063 4 0.24918871992089964 0.8596689864501798 5 0.1310450071410085 0.565110493886058 6 0.07630136669239398 0.3948448303962278 7 0.055475993268541204 0.33492380438042807 8 0.04052724308557664 0.27962753689699155 9 0.028745682845980343 0.22313003213367644 >10 0.14215816766481723 2.354750735303917 >50 0.010451488135098401 0.6200928288750286 >100 0.01219338352505055 1.9568016908365915 >500 0.0017419119321408114 1.0261002340402117 >1k 2.1773899151760143E-4 0.28375502445731327 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1511 0.2833652767828008 No Hit GGTATCAACGCAGAGTACTTTTTTT 947 0.17759557717624908 No Hit TATCAACGCAGAGTACTTTTTTTTT 782 0.14665231399348128 No Hit GTCCTACAGTGGACATTTCTAAATT 720 0.1350251484338958 No Hit CTGTAGGACGTGGAATATGGCAAGA 658 0.12339798287431035 No Hit GTCCTACAGTGTGCATTTCTCATTT 634 0.11889714459318047 No Hit CTTTAGGACGTGAAATATGGCGAGG 583 0.10933286324577952 No Hit GTCCTAAAGTGTGTATTTCTCATTT 583 0.10933286324577952 No Hit GTACATGGGAAGCAGTGGTATCAAC 557 0.10445695510788884 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTGG 45 6.544713E-4 14.838656 1 GTCTTAC 40 0.0051311506 14.308703 1 TAATACC 40 0.0051795165 14.288492 4 TTAGTCT 40 0.0051795165 14.288492 4 TAAGACC 40 0.0051795165 14.288492 4 TAGGACC 160 0.0 13.693138 4 GTAATAG 50 0.0014515981 13.35479 1 TCTAATC 50 0.0014661094 13.337181 3 TCAGGAC 125 9.640644E-11 12.95612 3 AGGACCT 280 0.0 12.5874815 5 GGACCTG 260 0.0 12.456635 6 TAGACAG 85 3.8127764E-6 12.327327 5 CAGGACC 85 3.8127764E-6 12.327327 4 ATAATAC 70 1.06029634E-4 12.248433 3 TTGGACC 70 1.0612294E-4 12.247279 4 TAGCACT 70 1.0612294E-4 12.247279 4 TTGGGCT 55 0.003002591 12.1235695 4 TGTAGGA 465 0.0 12.104562 2 GTCTATA 95 9.85312E-7 12.049436 1 GGCGAGG 135 3.765308E-10 11.945781 19 >>END_MODULE