FastQCFastQC Report
Thu 26 May 2016
SRR1926920_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926920_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667442
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13060.195672432960467No Hit
GTATCAACGCAGAGTACTTTTTTTT11700.17529613060011207No Hit
GTCCTAAAGTGTGTATTTCTCATTT10870.16286059313018958No Hit
GTCCTACAGTGTGCATTTCTCATTT10220.15312191920796114No Hit
CTGTAGGACGTGGAATATGGCAAGA10160.15222296469206315No Hit
CTTTAGGACGTGAAATATGGCGAGG9340.13993725297479032No Hit
CTGTAGGACCTGGAATATGGCGAGA7290.10922297368160828No Hit
GGTATCAACGCAGAGTACTTTTTTT7000.10487802685476791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3000.014.2840914
GTATAAT655.1998548E-513.2181411
GGTATCA3400.013.1965441
AGGACCT5000.012.941175
CTAGACA905.2354517E-712.696974
TTATACT604.0049985E-412.696974
GGACTGC604.0305045E-412.6874226
GGACCTG5000.012.3702366
CCTAGAC853.7660375E-612.3421063
TTAGAGT550.003010897712.1198354
TGTAGGA7050.011.7691652
TCCCGTT658.1410067E-411.67021811
GTATTGG855.054535E-511.2311011
GGCGAGA4250.011.15535719
AAGGTGC600.005798571711.1014956
CTGTAGG7150.011.0818761
GTAGGAC6800.011.0798453
GAAGGAC2200.010.8375933
GCATTTC5600.010.83663313
TGGCGAG6400.010.6673118
Produced by FastQC (version 0.11.5)