##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926920_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 667442 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.077320576169914 31.0 28.0 31.0 16.0 34.0 2 28.253873744834756 31.0 28.0 33.0 16.0 34.0 3 27.898842146583522 31.0 28.0 33.0 16.0 34.0 4 31.605141720179432 35.0 32.0 37.0 18.0 37.0 5 31.664643519586722 35.0 32.0 37.0 17.0 37.0 6 31.18325187806581 35.0 32.0 37.0 11.0 37.0 7 31.111357990656867 35.0 32.0 37.0 11.0 37.0 8 30.727393541311454 35.0 32.0 37.0 10.0 37.0 9 32.877069468208475 37.0 33.0 39.0 17.0 39.0 10 33.066560090614615 37.0 33.0 39.0 17.0 39.0 11 33.21884448386526 38.0 33.0 39.0 17.0 39.0 12 33.093326461325475 38.0 33.0 39.0 16.0 39.0 13 33.19316584811864 38.0 34.0 39.0 17.0 39.0 14 34.2185807905406 39.0 34.0 41.0 17.0 41.0 15 33.79681530380168 39.0 33.0 41.0 10.0 41.0 16 33.898840648325994 39.0 33.0 41.0 11.0 41.0 17 34.12353582783223 39.0 34.0 41.0 13.0 41.0 18 34.1732315317286 39.0 34.0 41.0 11.0 41.0 19 33.56260019597208 39.0 33.0 41.0 10.0 41.0 20 33.82369404382703 39.0 34.0 41.0 9.0 41.0 21 33.79594631443631 39.0 34.0 41.0 2.0 41.0 22 33.82241902667198 39.0 34.0 41.0 2.0 41.0 23 33.819047947237365 39.0 34.0 41.0 2.0 41.0 24 32.57673325921953 39.0 32.0 41.0 2.0 41.0 25 33.13123836977595 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.45429556034793E-4 1101 2 -0.2048299684833239 1101 3 -0.9813089766298155 1101 4 -0.24777557358124014 1101 5 -0.1721044029453047 1101 6 -1.8064707155388575 1101 7 -0.5650010745696576 1101 8 -0.27578631531110886 1101 9 -0.3808001665528664 1101 10 -8.785263654979751 1101 11 -3.9143727763530265 1101 12 -1.7068153676240811 1101 13 -0.9782570358227005 1101 14 -0.7354185998724034 1101 15 -1.9641099681408463 1101 16 -0.6450172053839935 1101 17 -0.24975405493421476 1101 18 -0.4170912431161966 1101 19 -0.9220097244274683 1101 20 -0.2646284883728498 1101 21 -0.2962712369450884 1101 22 -0.06925601966447914 1101 23 -0.24452222701245319 1101 24 -0.24617412072789335 1101 25 -0.3767277536927267 1102 1 0.01085438997360555 1102 2 -0.04932975099745818 1102 3 -0.16987574478988776 1102 4 -0.12819967101490448 1102 5 0.06664287848140305 1102 6 -0.6356442692578668 1102 7 -0.05813722072015537 1102 8 0.49937244936918646 1102 9 0.4871511166137239 1102 10 0.4898233393656142 1102 11 0.47993561860130285 1102 12 0.4863512265472991 1102 13 0.31366771406855776 1102 14 -0.05115370209076531 1102 15 0.5048221762697693 1102 16 0.4812768355033441 1102 17 0.4019880068317718 1102 18 0.28505099263848166 1102 19 0.8407849689174611 1102 20 0.7873615072774314 1102 21 0.6029458139466044 1102 22 0.6149327580649668 1102 23 0.25680443675440046 1102 24 -3.178901393455167 1102 25 -1.149263638860191 1103 1 0.041369740850800696 1103 2 0.07883910865166399 1103 3 0.4520760095960803 1103 4 0.25377401319562054 1103 5 0.12005296620070283 1103 6 0.5933140640754679 1103 7 0.41440663892896623 1103 8 0.5890983265621692 1103 9 0.4387848885435517 1103 10 0.2625755323480732 1103 11 0.154420267724106 1103 12 0.1003643844420381 1103 13 0.17536727547206254 1103 14 0.2849756838741513 1103 15 0.41863796574345 1103 16 0.3599500811173826 1103 17 -0.027112870361214902 1103 18 0.013943536498132403 1103 19 -1.6118685398544663 1103 20 -0.1571560365822151 1103 21 -0.14974058956217107 1103 22 -0.09241373316310586 1103 23 -0.030860036929809098 1103 24 1.4592236065448319 1103 25 0.8334995190345467 1104 1 -0.09280445593634923 1104 2 -0.19391440540456273 1104 3 -1.438812544620788 1104 4 -0.4784047016638162 1104 5 -0.3138200257671677 1104 6 -1.4960057150409973 1104 7 -0.06481022854091378 1104 8 0.16245425025694615 1104 9 0.2484108925596118 1104 10 0.272226636765744 1104 11 0.25516072957150016 1104 12 0.27097181416090876 1104 13 0.17216697426724892 1104 14 0.037146868613106676 1104 15 0.4951941906430477 1104 16 -6.297381597235585E-4 1104 17 0.006697370602644526 1104 18 0.05150628750214992 1104 19 -0.9366426362400091 1104 20 -0.014234349835227533 1104 21 0.09344465140168978 1104 22 0.08316357607384361 1104 23 0.0693282160822406 1104 24 1.3535885663841896 1104 25 0.5245135752594479 1105 1 0.4984214207581168 1105 2 0.6674924932925101 1105 3 1.0163407398178599 1105 4 0.795792384595817 1105 5 0.9461463541517148 1105 6 0.7281690375941707 1105 7 0.9914754903026832 1105 8 1.268369470223206 1105 9 0.27606869357499875 1105 10 0.7307369441322535 1105 11 0.5355264825536352 1105 12 0.5254574827538505 1105 13 0.6640351420725281 1105 14 0.3445761886738836 1105 15 0.8349115506491103 1105 16 0.38912191991248335 1105 17 0.6282640743590804 1105 18 0.7117640015759221 1105 19 -0.04721900327717776 1105 20 0.7201321130371099 1105 21 0.5677918914043971 1105 22 0.8223806230103463 1105 23 0.5200616363571839 1105 24 -3.9896064580033297 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11 -0.20316813484760132 1107 12 0.17592694626201677 1107 13 -0.3125314634003402 1107 14 -0.14118962275295388 1107 15 -1.3108996208045198 1107 16 -0.5149540979132823 1107 17 -0.3884321087390532 1107 18 -0.547792986746181 1107 19 0.11809560455700563 1107 20 0.3036871879479506 1107 21 -0.07245634129312606 1107 22 -0.0672839112046475 1107 23 -0.450379695683516 1107 24 -4.140068554681676 1107 25 -2.004099392933803 1108 1 0.31943832563480967 1108 2 -0.0942488184921153 1108 3 0.4740497362240603 1108 4 0.3232856186114823 1108 5 0.39787977148761655 1108 6 -3.0018725091031264 1108 7 -1.4720698278666475 1108 8 0.07127716279685359 1108 9 -0.07457544350029366 1108 10 0.19550388502376848 1108 11 0.32272499048362135 1108 12 0.30202057399716153 1108 13 0.38034591828315456 1108 14 0.27761551301664156 1108 15 -0.8065796358039421 1108 16 0.07878268911222364 1108 17 -0.049143012205185244 1108 18 0.08697504842591997 1108 19 0.1988140842590056 1108 20 -1.3404813105989817 1108 21 -0.4096878010386362 1108 22 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-0.3443513581584341 2215 1 0.7957600673406766 2215 2 0.4494915537614439 2215 3 0.6627279200608633 2215 4 1.0469198643504534 2215 5 1.111718741792373 2215 6 1.6116782286035551 2215 7 1.1567148795905027 2215 8 0.157368037141012 2215 9 0.4185868916801354 2215 10 0.24435037551887717 2215 11 0.2581779309690475 2215 12 0.05223029547710922 2215 13 0.10457582266339926 2215 14 0.24840507509171772 2215 15 0.2636443814833953 2215 16 0.4872018276243679 2215 17 0.038329459265249 2215 18 -0.7229585521816517 2215 19 0.5690250301483815 2215 20 -2.717888143796362 2215 21 -1.4179087175918212 2215 22 -0.5260019139443983 2215 23 -0.4048361917629961 2215 24 -0.11684835667158922 2215 25 -0.02358987360873499 2216 1 -0.8382010889604139 2216 2 -1.5063195628050856 2216 3 -0.533682302604543 2216 4 -0.8427496554434342 2216 5 -0.929741870372979 2216 6 -2.153108343645016 2216 7 -9.40246431063429 2216 8 -10.546622122860203 2216 9 -5.96505157947561 2216 10 -3.715737901013437 2216 11 -2.818735022498487 2216 12 -2.565427035676308 2216 13 -2.081179463045828 2216 14 -2.434634593668818 2216 15 -3.0976870553892617 2216 16 -2.4594981789558794 2216 17 -2.739202543508579 2216 18 -2.5343332668267493 2216 19 -1.882506395588475 2216 20 -2.7787149264103377 2216 21 -5.457400317654589 2216 22 -4.007574693997142 2216 23 -3.649940235577315 2216 24 -3.879848139017298 2216 25 -3.3753445215402635 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42218.0 3 10.0 4 318.0 5 1957.0 6 2559.0 7 2132.0 8 1633.0 9 1458.0 10 1324.0 11 1326.0 12 1287.0 13 1270.0 14 1304.0 15 1305.0 16 1350.0 17 1454.0 18 1678.0 19 2117.0 20 2656.0 21 3543.0 22 4443.0 23 5089.0 24 6024.0 25 6850.0 26 7803.0 27 8603.0 28 9355.0 29 11352.0 30 13495.0 31 16063.0 32 20739.0 33 27138.0 34 36090.0 35 50955.0 36 73798.0 37 119171.0 38 177575.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6359010711089 18.955563698839985 13.103362308741282 25.305172921309843 2 13.92982050770378 20.069138217920422 42.9837762250353 23.0172650493405 3 18.495789795881624 24.9479351094413 30.20558879839634 26.350686296280735 4 11.650211777053112 17.459574946587203 38.97897222534578 31.911241051013906 5 12.4697282133052 38.09464579012361 36.71638033352915 12.719245663042045 6 31.13114523172278 37.57241805108901 18.723943737955302 12.572492979232916 7 26.94485062615436 31.59190334995965 23.467601226910887 17.995644796975103 8 23.95899390198088 37.592078886547995 21.954972635552444 16.493954575918686 9 25.979889708086073 15.832883773901576 20.792573278187376 37.39465323982497 10 14.600606703716359 29.506513671816453 34.95515668183426 20.937722942632927 11 33.48682830341355 22.942577996223815 25.15209638265352 18.418497317709114 12 23.009559031779023 25.24279700956203 32.125929267060975 19.621714691597973 13 28.496911647031673 22.72674145046198 26.044510643654117 22.73183625885223 14 20.269549372430077 23.11294460361797 27.883554910869485 28.73395111308247 15 22.63123509032714 31.86788980927606 23.28021793596183 22.22065716443496 16 21.162505975420977 28.642758988606616 28.89481310981138 21.29992192616103 17 21.110340208023953 29.230497192530798 28.296322492571107 21.362840106874142 18 20.745304944474498 28.122981759224157 30.553187153387046 20.5785261429143 19 23.500506524879665 27.165019211979164 27.593017749351105 21.741456513790062 20 23.139882847796542 27.844552302659675 28.519473669225594 20.496091180318192 21 22.4638908160773 27.218959737829117 27.47549640294238 22.841653043151204 22 22.453689135041014 28.73191695962166 27.70275332739931 21.111640577938015 23 21.613529697362033 28.38122553691416 28.225381069692446 21.77986369603136 24 23.08089980579731 27.36504711213407 28.563829149584024 20.990223932484593 25 22.098299243275644 27.81493968682101 28.50513223945456 21.58162883044879 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 233.0 1 233.0 2 314.5 3 396.0 4 396.0 5 396.0 6 596.0 7 796.0 8 796.0 9 796.0 10 1188.0 11 1580.0 12 1580.0 13 1580.0 14 2546.0 15 3512.0 16 3512.0 17 3512.0 18 6071.5 19 8631.0 20 8631.0 21 8631.0 22 13569.0 23 18507.0 24 18507.0 25 18507.0 26 26527.0 27 34547.0 28 34547.0 29 34547.0 30 45004.0 31 55461.0 32 55461.0 33 55461.0 34 62383.5 35 69306.0 36 69306.0 37 69306.0 38 73968.5 39 78631.0 40 78631.0 41 78631.0 42 81777.5 43 84924.0 44 84924.0 45 84924.0 46 85879.5 47 86835.0 48 86835.0 49 86835.0 50 82380.0 51 77925.0 52 77925.0 53 77925.0 54 68061.5 55 58198.0 56 58198.0 57 58198.0 58 49306.0 59 40414.0 60 40414.0 61 40414.0 62 32565.5 63 24717.0 64 24717.0 65 24717.0 66 19080.5 67 13444.0 68 13444.0 69 13444.0 70 9593.5 71 5743.0 72 5743.0 73 5743.0 74 4064.5 75 2386.0 76 2386.0 77 2386.0 78 1646.0 79 906.0 80 906.0 81 906.0 82 579.5 83 253.0 84 253.0 85 253.0 86 165.0 87 77.0 88 77.0 89 77.0 90 47.0 91 17.0 92 17.0 93 17.0 94 10.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2382229467129728 2 0.14248429076983468 3 0.1447316770595797 4 0.06996862648739516 5 0.02307316590804894 6 0.020376302360354908 7 0.30429610363147663 8 0.2966549902463435 9 0.01827874182325955 10 0.03445992310942374 11 0.015282226770266182 12 0.01722996155471187 13 0.01438327225436817 14 0.015881529780864854 15 0.01348431773847016 16 0.018728219081208555 17 0.01722996155471187 18 0.013634143491119828 19 0.022473862897450264 20 0.015731704028215186 21 0.013634143491119828 22 0.015581878275565517 23 0.016480832791463527 24 0.014682923759667506 25 0.013783969243769498 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 667442.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.3114195890911 #Duplication Level Percentage of deduplicated Percentage of total 1 93.3776636994382 79.66181048111814 2 4.949760553064208 8.445421988159845 3 0.8382470261849465 2.1453613131051545 4 0.301703817594497 1.0295512389773898 5 0.13916458160178638 0.5936164006485154 6 0.09331021386342546 0.47762560841103097 7 0.060023081946409304 0.35844580302723666 8 0.036844070459273515 0.25145759634568954 9 0.024894984119342628 0.19114437922820984 >10 0.14797978601902864 2.35484722274209 >50 0.016346768443886526 0.9943353699541496 >100 0.012303740108430675 2.1349871529218234 >500 8.788385782623308E-4 0.5215258683736724 >1k 8.788385782623308E-4 0.8398695769870439 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1306 0.195672432960467 No Hit GTATCAACGCAGAGTACTTTTTTTT 1170 0.17529613060011207 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1087 0.16286059313018958 No Hit GTCCTACAGTGTGCATTTCTCATTT 1022 0.15312191920796114 No Hit CTGTAGGACGTGGAATATGGCAAGA 1016 0.15222296469206315 No Hit CTTTAGGACGTGAAATATGGCGAGG 934 0.13993725297479032 No Hit CTGTAGGACCTGGAATATGGCGAGA 729 0.10922297368160828 No Hit GGTATCAACGCAGAGTACTTTTTTT 700 0.10487802685476791 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 1.4982575264966844E-4 0.0 0.0 0.0 0.0 13 1.4982575264966844E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 300 0.0 14.284091 4 GTATAAT 65 5.1998548E-5 13.218141 1 GGTATCA 340 0.0 13.196544 1 AGGACCT 500 0.0 12.94117 5 CTAGACA 90 5.2354517E-7 12.69697 4 TTATACT 60 4.0049985E-4 12.69697 4 GGACTGC 60 4.0305045E-4 12.687422 6 GGACCTG 500 0.0 12.370236 6 CCTAGAC 85 3.7660375E-6 12.342106 3 TTAGAGT 55 0.0030108977 12.119835 4 TGTAGGA 705 0.0 11.769165 2 TCCCGTT 65 8.1410067E-4 11.670218 11 GTATTGG 85 5.054535E-5 11.231101 1 GGCGAGA 425 0.0 11.155357 19 AAGGTGC 60 0.0057985717 11.101495 6 CTGTAGG 715 0.0 11.081876 1 GTAGGAC 680 0.0 11.079845 3 GAAGGAC 220 0.0 10.837593 3 GCATTTC 560 0.0 10.836633 13 TGGCGAG 640 0.0 10.66731 18 >>END_MODULE