FastQCFastQC Report
Thu 26 May 2016
SRR1926920_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926920_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667442
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17680.2648919306846138No Hit
GTCCTACAGTGGACATTTCTAAATT13410.20091633430320538No Hit
CTGTAGGACGTGGAATATGGCAAGA12710.19042853161772857No Hit
GGTATCAACGCAGAGTACTTTTTTT11670.17484665334216307No Hit
CTTTAGGACGTGAAATATGGCGAGG11250.168553971730877No Hit
GTCCTAAAGTGTGTATTTCTCATTT10770.16136233560369292No Hit
GTCCTACAGTGTGCATTTCTCATTT10580.15851564630334922No Hit
TATCAACGCAGAGTACTTTTTTTTT9650.14458185130693002No Hit
CTGTAGGACCTGGAATATGGCGAGA8070.12090938238828242No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCA400.00531233914.2348269
TAGAGTG903.5284756E-813.7508875
TAGGACC3500.012.78379254
GTAAGGC701.06733205E-412.2407213
TCCAACG951.053093E-611.98452818
GGATAAA802.7509708E-511.9204171
CTAGGAC907.2655366E-611.6362413
TATAGAA1406.366463E-1011.5772172
GTCTTTA600.00570521211.1257211
ATAATAC600.00577596811.1073213
CCTATAC600.00577596811.1073213
AGAGGTA600.00577920211.1064855
GGCGAGG2650.011.09887919
AGGACCT5750.011.0926895
TGCGTTG951.3752633E-510.9866412
TTAGACA803.6926655E-410.70982554
TGGCGAG7550.010.68157418
GTCCTAG1252.1166488E-710.6806921
GTATTAA909.13485E-510.5959251
GTAGGAC8400.010.4272813
Produced by FastQC (version 0.11.5)