##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926920_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 667442 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.77259597088586 31.0 30.0 33.0 25.0 34.0 2 30.052437515169856 31.0 30.0 34.0 26.0 34.0 3 30.11263450606944 31.0 30.0 34.0 26.0 34.0 4 33.682655571570265 35.0 35.0 37.0 28.0 37.0 5 33.63598934439247 37.0 35.0 37.0 28.0 37.0 6 33.58264987819166 37.0 35.0 37.0 28.0 37.0 7 33.579064547930756 37.0 35.0 37.0 28.0 37.0 8 33.60252875905322 37.0 35.0 37.0 28.0 37.0 9 35.11819453975027 39.0 35.0 39.0 29.0 39.0 10 35.01255389981451 39.0 35.0 39.0 28.0 39.0 11 35.009659266273324 39.0 35.0 39.0 28.0 39.0 12 34.913084582630404 39.0 35.0 39.0 27.0 39.0 13 34.91045963544398 39.0 35.0 39.0 27.0 39.0 14 36.01510543238214 40.0 36.0 41.0 27.0 41.0 15 36.00950794226315 40.0 36.0 41.0 27.0 41.0 16 35.99529846788185 40.0 36.0 41.0 27.0 41.0 17 35.97931655484671 40.0 36.0 41.0 27.0 41.0 18 35.95588680364736 40.0 36.0 41.0 27.0 41.0 19 36.01288501472787 40.0 36.0 41.0 27.0 41.0 20 35.96996443136632 40.0 36.0 41.0 27.0 41.0 21 35.91870754312734 40.0 36.0 41.0 27.0 41.0 22 35.83165128955025 40.0 36.0 41.0 27.0 41.0 23 35.76256513674596 40.0 36.0 41.0 27.0 41.0 24 35.6994120837466 40.0 36.0 41.0 27.0 41.0 25 35.579873606995065 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.08179263954830063 1101 2 0.025723885097917787 1101 3 0.09887767545729531 1101 4 0.05937760058147035 1101 5 -0.04506617616156916 1101 6 -0.17773904757654435 1101 7 -0.24446557671120672 1101 8 -0.24841237836196228 1101 9 -0.03256644421630028 1101 10 -0.09192945099088234 1101 11 -0.08013999507672764 1101 12 -0.17610854664067688 1101 13 0.05385492669480385 1101 14 5.353351503742942E-4 1101 15 0.0490986989715978 1101 16 -0.04547276127355815 1101 17 -0.03992335129216684 1101 18 -0.053463289948425086 1101 19 0.012154441982836772 1101 20 -0.031776504331659794 1101 21 -0.13214491927799088 1101 22 -0.19324843646909073 1101 23 -0.06082678525128671 1101 24 -0.1692520452203965 1101 25 -0.015540417803485695 1102 1 -0.5420731963121135 1102 2 -0.3491651971117413 1102 3 -0.23325151114557485 1102 4 -0.2880321427852124 1102 5 -0.41262598477401013 1102 6 -0.15738019590190078 1102 7 -2.72449264492991E-4 1102 8 -0.02318619306400649 1102 9 0.02024129828043897 1102 10 -0.08156842445760759 1102 11 -0.27313260055737487 1102 12 -0.08445782893733167 1102 13 -0.08410723076495685 1102 14 -0.06775305110452479 1102 15 0.05853323572670632 1102 16 0.022822149557235605 1102 17 -0.061493599226047024 1102 18 -0.11589478190144575 1102 19 -0.06217570155783392 1102 20 -0.16919494713287264 1102 21 -0.08267340992610173 1102 22 -0.09829193364177513 1102 23 -0.04120956037090906 1102 24 0.13795543629330353 1102 25 0.053757102857673544 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0.368283646688468 2214 5 0.5612336643487978 2214 6 0.3892864707647661 2214 7 0.2023591296828755 2214 8 0.3275155613219525 2214 9 0.3169079649471058 2214 10 0.21036140010379967 2214 11 0.3412533643549054 2214 12 0.41747199562407644 2214 13 0.29571733063854566 2214 14 0.21049256293056828 2214 15 0.17046306028810676 2214 16 0.17843618464495137 2214 17 0.19587482182657823 2214 18 0.32778942862486815 2214 19 0.5120348247579543 2214 20 0.5255418949723918 2214 21 0.4448704497230267 2214 22 0.30276069793716687 2214 23 0.32370272033085 2214 24 0.4456747345389189 2214 25 0.4577921905769671 2215 1 0.7060399008019296 2215 2 0.6197772188082986 2215 3 0.6653194671984899 2215 4 0.6618341396874357 2215 5 0.748230347673875 2215 6 0.5871824082131738 2215 7 0.4341924555124237 2215 8 0.5039904311312071 2215 9 0.2943208084330635 2215 10 0.48556137609167394 2215 11 0.19972589298186705 2215 12 0.3108363946455839 2215 13 0.3490204987384402 2215 14 0.21846458880363429 2215 15 0.22567294127800608 2215 16 0.15698360109026055 2215 17 0.46890845381073376 2215 18 0.3998820854165501 2215 19 0.30629667699333396 2215 20 0.40487645892718405 2215 21 0.45455859223499573 2215 22 0.32685686650229684 2215 23 0.5099407705462085 2215 24 0.37597803770702143 2215 25 0.22942861697229944 2216 1 0.12687194761278775 2216 2 0.06611531185297892 2216 3 -1.9810327986839482 2216 4 -1.4755411519946264 2216 5 -0.5135976446930357 2216 6 -0.2265460098450447 2216 7 -0.3066167122889141 2216 8 -0.17259047299460661 2216 9 -0.08666531737111427 2216 10 -0.28147766501462 2216 11 -0.08606938037674183 2216 12 -0.07141516310114326 2216 13 -0.09014006432816046 2216 14 -0.20619412420146688 2216 15 -0.06717838059219616 2216 16 -0.11451735304542865 2216 17 -0.24215293988539344 2216 18 -0.20009463063571076 2216 19 -0.1650817833303151 2216 20 -0.1531385339104716 2216 21 -0.14926503692258564 2216 22 -0.18812331683348305 2216 23 -0.1758271694824458 2216 24 -0.18423880671065263 2216 25 -0.23574416510513174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18284.0 3 11.0 4 302.0 5 2122.0 6 2738.0 7 2041.0 8 1563.0 9 1345.0 10 1143.0 11 1041.0 12 1017.0 13 923.0 14 831.0 15 839.0 16 954.0 17 985.0 18 1091.0 19 1287.0 20 1647.0 21 2203.0 22 2727.0 23 3180.0 24 3832.0 25 4512.0 26 5371.0 27 5847.0 28 6806.0 29 8235.0 30 10491.0 31 13383.0 32 17614.0 33 22894.0 34 30883.0 35 43211.0 36 63759.0 37 110550.0 38 271780.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.23280047880407 19.73521774935087 11.142394710355038 27.889587061490023 2 13.82980797933539 21.07517171756567 40.70085067509491 24.394169628004025 3 18.025903498006212 25.473721121555826 29.59286582359667 26.90750955684129 4 11.936018410588485 18.018644316659724 38.172305608577226 31.87303166417457 5 12.655940497631113 38.976359075939236 35.316495905016765 13.051204521412881 6 30.900617149386235 38.51147476903879 17.895979132683358 12.691928948891611 7 27.411890939280504 32.24826089693061 22.124298289519825 18.21554987426907 8 24.524069603837113 38.62884096994652 19.846054029431638 17.001035396784726 9 26.004492213546467 15.960244359634808 20.202224529763537 37.83303889705519 10 14.875324760942982 30.02723946456023 33.981906283056695 21.115529491440096 11 33.53446028354555 23.179743664276316 24.81188362106424 18.473912431113895 12 23.215524461484755 25.498136902808806 31.520577972153173 19.765760663553262 13 28.698268293194534 22.756407375111248 25.743069726744515 22.802254604949702 14 20.460054956086072 23.264942871440514 27.453921089772592 28.821081082700818 15 22.27759265474421 32.1112216522031 23.340784868389512 22.270400824663184 16 21.40508064455715 28.80969682440987 28.448612973547988 21.336609557484998 17 20.931382390574104 29.72485833890189 28.06133303767541 21.282426232848593 18 20.934922501816654 28.277959651501643 30.249537408137122 20.537580438544577 19 23.037276758959607 27.4805225937912 27.647129329977226 21.835071317271964 20 23.03055728273165 28.141616786653383 28.378643613235745 20.44918231737922 21 22.30669989166413 27.16353321775509 27.511017143439386 23.01874974714139 22 22.542516866659376 28.872514574321922 27.291550863671844 21.293417695346857 23 21.64284430061129 28.584442047225217 28.06244756082944 21.710266091334052 24 22.58375284669783 27.51183626992689 28.709397099364736 21.19501378401055 25 22.05013102870645 27.947846229684558 28.292761132195903 21.70926160941309 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 290.0 1 290.0 2 294.0 3 298.0 4 298.0 5 298.0 6 520.0 7 742.0 8 742.0 9 742.0 10 1135.0 11 1528.0 12 1528.0 13 1528.0 14 2442.0 15 3356.0 16 3356.0 17 3356.0 18 5877.5 19 8399.0 20 8399.0 21 8399.0 22 13053.0 23 17707.0 24 17707.0 25 17707.0 26 25571.5 27 33436.0 28 33436.0 29 33436.0 30 43844.5 31 54253.0 32 54253.0 33 54253.0 34 60851.5 35 67450.0 36 67450.0 37 67450.0 38 72335.5 39 77221.0 40 77221.0 41 77221.0 42 81005.5 43 84790.0 44 84790.0 45 84790.0 46 86315.5 47 87841.0 48 87841.0 49 87841.0 50 83645.5 51 79450.0 52 79450.0 53 79450.0 54 69666.5 55 59883.0 56 59883.0 57 59883.0 58 50841.5 59 41800.0 60 41800.0 61 41800.0 62 33559.0 63 25318.0 64 25318.0 65 25318.0 66 19570.0 67 13822.0 68 13822.0 69 13822.0 70 9965.0 71 6108.0 72 6108.0 73 6108.0 74 4293.5 75 2479.0 76 2479.0 77 2479.0 78 1677.5 79 876.0 80 876.0 81 876.0 82 581.0 83 286.0 84 286.0 85 286.0 86 187.5 87 89.0 88 89.0 89 89.0 90 53.5 91 18.0 92 18.0 93 18.0 94 10.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11701391281939105 2 0.1191114733564864 3 0.017829264565310546 4 0.0 5 0.0059930301059867375 6 0.34265149630979175 7 0.021574908381552255 8 0.009738673922228448 9 0.009738673922228448 10 0.004195121074190716 11 0.005393727095388064 12 7.491287632483422E-4 13 0.0011986060211973475 14 0.0 15 0.002696863547694032 16 0.001048780268547679 17 5.993030105986738E-4 18 0.001048780268547679 19 2.996515052993369E-4 20 0.001048780268547679 21 0.010937279943425797 22 1.4982575264966844E-4 23 2.996515052993369E-4 24 2.996515052993369E-4 25 0.004644598332139722 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 667442.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.57169127582651 #Duplication Level Percentage of deduplicated Percentage of total 1 91.79838979040997 75.79948301391717 2 6.127461460172346 10.119097119877521 3 1.050877643477669 2.603182330377184 4 0.3648487505142722 1.2050471355934211 5 0.1877403628131595 0.7751019639109933 6 0.10646256272600178 0.5274476317106841 7 0.07641393684168027 0.441673960144321 8 0.048230618206366614 0.31859869732626894 9 0.03413778328498658 0.2536933052024084 >10 0.17055558276971863 2.5617002741478774 >50 0.019076019985391945 1.1060421371668598 >100 0.013443749093602875 2.3502047636016745 >500 0.0010900337099007513 0.6175906689210874 >1k 0.0012717059948842099 1.3211369981025058 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1768 0.2648919306846138 No Hit GTCCTACAGTGGACATTTCTAAATT 1341 0.20091633430320538 No Hit CTGTAGGACGTGGAATATGGCAAGA 1271 0.19042853161772857 No Hit GGTATCAACGCAGAGTACTTTTTTT 1167 0.17484665334216307 No Hit CTTTAGGACGTGAAATATGGCGAGG 1125 0.168553971730877 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1077 0.16136233560369292 No Hit GTCCTACAGTGTGCATTTCTCATTT 1058 0.15851564630334922 No Hit TATCAACGCAGAGTACTTTTTTTTT 965 0.14458185130693002 No Hit CTGTAGGACCTGGAATATGGCGAGA 807 0.12090938238828242 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTGCA 40 0.005312339 14.234826 9 TAGAGTG 90 3.5284756E-8 13.750887 5 TAGGACC 350 0.0 12.7837925 4 GTAAGGC 70 1.06733205E-4 12.240721 3 TCCAACG 95 1.053093E-6 11.984528 18 GGATAAA 80 2.7509708E-5 11.920417 1 CTAGGAC 90 7.2655366E-6 11.636241 3 TATAGAA 140 6.366463E-10 11.577217 2 GTCTTTA 60 0.005705212 11.125721 1 ATAATAC 60 0.005775968 11.107321 3 CCTATAC 60 0.005775968 11.107321 3 AGAGGTA 60 0.005779202 11.106485 5 GGCGAGG 265 0.0 11.098879 19 AGGACCT 575 0.0 11.092689 5 TGCGTTG 95 1.3752633E-5 10.98664 12 TTAGACA 80 3.6926655E-4 10.7098255 4 TGGCGAG 755 0.0 10.681574 18 GTCCTAG 125 2.1166488E-7 10.680692 1 GTATTAA 90 9.13485E-5 10.595925 1 GTAGGAC 840 0.0 10.427281 3 >>END_MODULE