FastQCFastQC Report
Thu 26 May 2016
SRR1926919_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926919_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660808
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16520.24999697340225904No Hit
GTCCTAAAGTGTGTATTTCTCATTT13010.1968801830486314No Hit
CTGTAGGACGTGGAATATGGCAAGA12330.18658975072941006No Hit
GTCCTACAGTGTGCATTTCTCATTT10740.16252829868887786No Hit
CTTTAGGACGTGAAATATGGCGAGG10220.1546591445624145No Hit
GTATCAACGCAGAGTACTTTTTTTT9480.14346073292090894No Hit
GTACATGGGAAGCAGTGGTATCAAC8240.12469582692703478No Hit
CTGTAGGACCTGGAATATGGCGAGA7640.11561603370419243No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG402.7241788E-416.6519815
TATGTCG508.830035E-515.17396216
CGAAATC551.9874173E-413.79346713
CCGGTTT707.388462E-613.54612812
TGTCGAG500.001517433213.27721718
CCAACGT655.5314988E-513.13131119
CTGGACT801.9484305E-613.08966354
TGCCGGT802.0314765E-613.04111110
TCCAACG1403.6379788E-1212.8707718
CTAGGAC603.9668093E-412.7113583
CCAACGA751.5017769E-512.64496719
GATATAC3400.012.3506521
GTATTAA701.04338826E-412.27045251
TAGGACC3200.012.1971864
CCCTTAT550.002961654212.1465091
ATTAGAG550.00298540212.1335693
TGGACTG802.8134074E-511.8942725
CGGTTTC802.913402E-511.8537613
GCCGGTT802.9156598E-511.85286311
TAGGACT1302.5174813E-911.7166214
Produced by FastQC (version 0.11.5)