FastQCFastQC Report
Thu 26 May 2016
SRR1926918_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926918_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663711
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17710.2668330041237828No Hit
CTGTAGGACGTGGAATATGGCAAGA14990.22585131179082463No Hit
GTCCTAAAGTGTGTATTTCTCATTT14630.22042726427616838No Hit
GTCCTACAGTGTGCATTTCTCATTT14060.2118391890446294No Hit
CTTTAGGACGTGAAATATGGCGAGG11210.16889881288693423No Hit
CTGTAGGACCTGGAATATGGCGAGA9480.1428332512192807No Hit
GTATCAACGCAGAGTACTTTTTTTT7360.11089163807741624No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG400.005196642614.2821224
ACTGTTC1850.013.3652678
TAGGACC4350.013.3518694
TAGGACT1301.2732926E-1113.1834984
CCAACGA604.152612E-412.64260319
CTAGAGT1301.891749E-1012.4510824
GTATTAA959.816104E-712.0544161
GTCCTAG1353.4197E-1012.0172111
TGTAGGA9850.011.9059032
TGACAGT907.276454E-611.6346446
GTGTAGA1655.456968E-1211.5673691
GTAGGAC9350.011.5228613
GTCCTAT751.9851557E-411.4516951
ATAGGAC752.0020094E-411.4412823
TATTAAG1001.8419869E-611.4412822
CCTATAC1001.8419869E-611.4412823
GATATAC2600.011.3782861
AGGACCT6800.011.3391115
CTGTAGG9500.011.2507891
TCCAACG855.4158587E-511.15523718
Produced by FastQC (version 0.11.5)