##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926918_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 663711 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.343443155228705 31.0 28.0 33.0 16.0 34.0 2 28.52728220565879 31.0 30.0 33.0 18.0 34.0 3 28.176855589254963 31.0 28.0 33.0 16.0 34.0 4 31.902373171455647 35.0 32.0 37.0 19.0 37.0 5 31.96670840169893 35.0 33.0 37.0 19.0 37.0 6 31.49072563208987 35.0 32.0 37.0 17.0 37.0 7 31.428462086661213 35.0 32.0 37.0 17.0 37.0 8 31.074344104587688 35.0 32.0 37.0 11.0 37.0 9 33.24446332816542 37.0 33.0 39.0 17.0 39.0 10 33.40355365513002 37.0 34.0 39.0 17.0 39.0 11 33.580951649136445 38.0 34.0 39.0 19.0 39.0 12 33.443711193576725 38.0 34.0 39.0 18.0 39.0 13 33.567591918771875 38.0 34.0 39.0 19.0 39.0 14 34.59753718109237 39.0 34.0 41.0 19.0 41.0 15 34.191503530904264 39.0 34.0 41.0 16.0 41.0 16 34.27813310311265 39.0 34.0 41.0 17.0 41.0 17 34.51355635208698 39.0 34.0 41.0 17.0 41.0 18 34.57367739874735 39.0 35.0 41.0 18.0 41.0 19 33.94537833484755 39.0 34.0 41.0 10.0 41.0 20 34.229642118331626 39.0 34.0 41.0 16.0 41.0 21 34.200882613065026 39.0 34.0 41.0 11.0 41.0 22 34.22809475811008 39.0 34.0 41.0 10.0 41.0 23 34.22629879571078 39.0 34.0 41.0 2.0 41.0 24 32.95647201869488 39.0 32.0 41.0 2.0 41.0 25 33.52570621851981 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.0013571297663403925 1101 2 -0.13271154317029854 1101 3 -0.8151582260041081 1101 4 -0.31755192819850464 1101 5 0.0013280431507141088 1101 6 -2.0711419869195424 1101 7 -0.5806172222190291 1101 8 -0.03709763414131473 1101 9 0.03067779182518393 1101 10 -9.568593851632347 1101 11 -3.99472011209658 1101 12 -1.369715476944421 1101 13 -0.49091076583808047 1101 14 -0.2015490535967075 1101 15 -1.5789045026215973 1101 16 0.017629929726815874 1101 17 0.1133387112951425 1101 18 0.147134157197506 1101 19 -0.5579826871487299 1101 20 0.21818626127496543 1101 21 0.2569636033717586 1101 22 0.29953681165797263 1101 23 0.18517155096451177 1101 24 0.13280998137408773 1101 25 0.28340185441297905 1102 1 -0.2319048144139586 1102 2 -0.37600427708444784 1102 3 -0.4248915018977506 1102 4 -0.27149467973620034 1102 5 -0.141974111155303 1102 6 -1.2210564095394894 1102 7 -0.34246759887593115 1102 8 0.20514192113150997 1102 9 0.1698603770135776 1102 10 0.090127578912373 1102 11 -0.33755780932941803 1102 12 -0.2034508528445187 1102 13 -0.4107538157587456 1102 14 -0.584469988988694 1102 15 0.012729977706186446 1102 16 -0.05807403287104762 1102 17 -0.12131478234714876 1102 18 -0.4423061127549346 1102 19 0.10950394171641165 1102 20 -0.09602720480474147 1102 21 -0.175888584329023 1102 22 -0.2299304916437208 1102 23 -0.7251537277573661 1102 24 -3.5967432697724995 1102 25 -1.9082777167792528 1103 1 -0.2003960243415719 1103 2 -0.30730417367183094 1103 3 -0.1049504470890632 1103 4 -0.43328785450352214 1103 5 -0.5673536354572697 1103 6 0.4865826804294855 1103 7 0.31531420050359316 1103 8 0.24233737097639008 1103 9 -0.1392781958923166 1103 10 -0.15895618530686306 1103 11 -0.3273147896810187 1103 12 -0.23750462740501632 1103 13 -0.19908783850952005 1103 14 -0.24309460119138748 1103 15 -0.022958750318629484 1103 16 -0.07179688705926424 1103 17 -0.17966845349502591 1103 18 -0.35699173840126264 1103 19 -2.049939698407684 1103 20 -0.7513715688171558 1103 21 -0.5212298459629423 1103 22 -0.5069749590687422 1103 23 -0.33037399663016487 1103 24 0.9606093672492193 1103 25 0.2748163887016162 1104 1 0.023216220556388834 1104 2 -0.05630417367183327 1104 3 -1.1784402430074294 1104 4 -0.09067560960556165 1104 5 -0.018904655865430442 1104 6 -1.3642132379378609 1104 7 -0.02703273827192021 1104 8 0.3434394117927191 1104 9 0.43906874288319386 1104 10 0.6361050391829366 1104 11 0.44135867970673104 1104 12 0.631066801166412 1104 13 0.20995297781700373 1104 14 0.2646605008494305 1104 15 0.7233065558038163 1104 16 0.21983576600196386 1104 17 0.22780093426007397 1104 18 0.19090622078240926 1104 19 -0.5865927596321754 1104 20 0.15262843118284763 1104 21 0.37397423566971355 1104 22 0.4224944286863561 1104 23 0.4809729421453426 1104 24 1.7459154896981985 1104 25 1.2280000621710059 1105 1 0.5163663518960533 1105 2 0.2946859253380687 1105 3 0.7014950974653935 1105 4 0.5514844227242008 1105 5 0.5635968595922378 1105 6 0.2245925814195857 1105 7 0.5942745965431939 1105 8 0.7185353907783707 1105 9 -0.023327700842813215 1105 10 0.13304381469313853 1105 11 0.22996243804175265 1105 12 0.4960597290306268 1105 13 0.26286265653997987 1105 14 0.2665093592046546 1105 15 0.7763778833447361 1105 16 0.30949024165360584 1105 17 0.12116322967328585 1105 18 -0.010427381965619986 1105 19 -0.58809811424927 1105 20 0.07094526286601166 1105 21 0.38802757977963154 1105 22 0.3970448429114555 1105 23 0.3156062013896346 1105 24 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-3.4296770333633972 2216 11 -2.5352476518718348 2216 12 -2.1736530372990117 2216 13 -1.9758334215483941 2216 14 -2.0783949545482727 2216 15 -3.0821848987285243 2216 16 -2.181408194479758 2216 17 -2.433830997664643 2216 18 -2.146747922146851 2216 19 -1.680441465192132 2216 20 -2.7318238656369402 2216 21 -4.816971895432907 2216 22 -3.458925489104079 2216 23 -3.382487070835115 2216 24 -4.095185685754316 2216 25 -3.108392091863756 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37492.0 3 6.0 4 315.0 5 1723.0 6 2213.0 7 1789.0 8 1431.0 9 1316.0 10 1184.0 11 1199.0 12 1121.0 13 1161.0 14 1127.0 15 1222.0 16 1286.0 17 1408.0 18 1656.0 19 1990.0 20 2604.0 21 3375.0 22 4039.0 23 5085.0 24 5784.0 25 6775.0 26 7763.0 27 8275.0 28 9441.0 29 11115.0 30 13279.0 31 16012.0 32 20714.0 33 27059.0 34 36778.0 35 50600.0 36 73767.0 37 120046.0 38 181561.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.69921159488571 18.84833356739346 12.717015265250527 25.735439572470302 2 13.862137536568845 19.85316638276621 43.091713677463936 23.19298240320101 3 18.838030028471373 24.319108711757153 29.88392672582234 26.95893453394913 4 11.679337287813587 17.06176286990323 38.702741498219275 32.55615834406391 5 12.417565856893122 38.12827777442884 36.856501296039546 12.597655072638494 6 31.91402788578042 37.17079287001706 18.614481798289237 12.300697445913281 7 27.60505998570438 31.02467581727594 23.209533639293923 18.160730557725756 8 23.982941863610467 38.04169934581261 21.68021434497391 16.29514444560301 9 26.174046574570397 15.25058734351948 20.601403596546618 37.97396248536351 10 14.564407727450174 29.439312204481705 35.03172040822786 20.96455965984026 11 34.21955997588909 22.272905364677516 25.08785412899337 18.419680530440026 12 23.380038457133555 24.654307741457153 32.48075636381172 19.484897437597574 13 29.36331292466776 22.251724288622636 25.666245232494532 22.718717554215072 14 20.019620430564473 22.975946135739626 27.783855791342297 29.220577642353607 15 22.63039799398134 32.54114282183775 22.958606275137395 21.869852909043512 16 21.179168939670216 28.169822750932056 29.46792126416169 21.183087045236036 17 20.996532450446896 29.135252516271564 28.709230674175423 21.15898435910612 18 20.87245065566409 27.907540965819873 30.901654907610283 20.318353470905752 19 23.826912295719374 26.85276724517292 27.19109996563926 22.129220493468445 20 23.283910022348238 27.971162786492197 28.35167054206909 20.393256649090475 21 22.478887757008785 27.098716689069857 27.0256300520196 23.396765501901758 22 22.657898226621175 29.15707241799178 27.320100302595574 20.864929052791474 23 21.511605127293222 28.65672271566911 28.106680972775195 21.72499118426248 24 23.38828167265176 27.171474081251613 28.683997570800173 20.756246675296456 25 22.15978444651398 27.802692610240598 28.47584517039033 21.561677772855088 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 150.0 1 150.0 2 202.0 3 254.0 4 254.0 5 254.0 6 383.5 7 513.0 8 513.0 9 513.0 10 794.5 11 1076.0 12 1076.0 13 1076.0 14 1857.5 15 2639.0 16 2639.0 17 2639.0 18 4826.0 19 7013.0 20 7013.0 21 7013.0 22 11731.5 23 16450.0 24 16450.0 25 16450.0 26 24740.0 27 33030.0 28 33030.0 29 33030.0 30 44375.5 31 55721.0 32 55721.0 33 55721.0 34 62877.5 35 70034.0 36 70034.0 37 70034.0 38 74912.5 39 79791.0 40 79791.0 41 79791.0 42 82518.0 43 85245.0 44 85245.0 45 85245.0 46 86285.5 47 87326.0 48 87326.0 49 87326.0 50 82134.5 51 76943.0 52 76943.0 53 76943.0 54 67585.5 55 58228.0 56 58228.0 57 58228.0 58 49572.0 59 40916.0 60 40916.0 61 40916.0 62 33269.0 63 25622.0 64 25622.0 65 25622.0 66 19607.0 67 13592.0 68 13592.0 69 13592.0 70 9790.0 71 5988.0 72 5988.0 73 5988.0 74 4071.5 75 2155.0 76 2155.0 77 2155.0 78 1433.0 79 711.0 80 711.0 81 711.0 82 469.5 83 228.0 84 228.0 85 228.0 86 148.5 87 69.0 88 69.0 89 69.0 90 39.5 91 10.0 92 10.0 93 10.0 94 7.0 95 4.0 96 4.0 97 4.0 98 3.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2241939639391241 2 0.13861454759677028 3 0.14162790732713484 4 0.0748819892995596 5 0.022750865964252513 6 0.0221481940181796 7 0.2968159334409103 8 0.29741860538698317 9 0.019586838247369714 10 0.03887234052170297 11 0.01672414650352337 12 0.017326818449596285 13 0.015669470597895772 14 0.01762815442263274 15 0.016272142543968686 16 0.018833498314778572 17 0.019586838247369714 18 0.016422810530486914 19 0.024558881802471257 20 0.018682830328260343 21 0.017326818449596285 22 0.0179294903956692 23 0.018833498314778572 24 0.01777882240915097 25 0.01762815442263274 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 663711.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.57193419642704 #Duplication Level Percentage of deduplicated Percentage of total 1 92.16043440384851 75.1770489070486 2 5.638887974708309 9.199499976278599 3 1.0098951801698801 2.4713730954631883 4 0.3897053758620235 1.2715608510324337 5 0.20271302367824512 0.8267846714120283 6 0.12215536886060288 0.5978669826262422 7 0.07525669802771343 0.42971840935499134 8 0.07240819029240293 0.4725180907051406 9 0.03950213859704218 0.29000392652304585 >10 0.25079606567452184 3.723133972760341 >50 0.020508649991632565 1.1791857233420173 >100 0.016258852765699244 2.9275816322294514 >500 5.542790713257088E-4 0.33955425565410685 >1k 9.237984522095145E-4 1.094169505569825 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1771 0.2668330041237828 No Hit CTGTAGGACGTGGAATATGGCAAGA 1499 0.22585131179082463 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1463 0.22042726427616838 No Hit GTCCTACAGTGTGCATTTCTCATTT 1406 0.2118391890446294 No Hit CTTTAGGACGTGAAATATGGCGAGG 1121 0.16889881288693423 No Hit CTGTAGGACCTGGAATATGGCGAGA 948 0.1428332512192807 No Hit GTATCAACGCAGAGTACTTTTTTTT 736 0.11089163807741624 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 3.0133597303645714E-4 0.0 12 0.0 0.0 0.0 3.0133597303645714E-4 1.5066798651822857E-4 13 0.0 0.0 0.0 3.0133597303645714E-4 1.5066798651822857E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGAACG 40 0.0051966426 14.282122 4 ACTGTTC 185 0.0 13.365267 8 TAGGACC 435 0.0 13.351869 4 TAGGACT 130 1.2732926E-11 13.183498 4 CCAACGA 60 4.152612E-4 12.642603 19 CTAGAGT 130 1.891749E-10 12.451082 4 GTATTAA 95 9.816104E-7 12.054416 1 GTCCTAG 135 3.4197E-10 12.017211 1 TGTAGGA 985 0.0 11.905903 2 TGACAGT 90 7.276454E-6 11.634644 6 GTGTAGA 165 5.456968E-12 11.567369 1 GTAGGAC 935 0.0 11.522861 3 GTCCTAT 75 1.9851557E-4 11.451695 1 ATAGGAC 75 2.0020094E-4 11.441282 3 TATTAAG 100 1.8419869E-6 11.441282 2 CCTATAC 100 1.8419869E-6 11.441282 3 GATATAC 260 0.0 11.378286 1 AGGACCT 680 0.0 11.339111 5 CTGTAGG 950 0.0 11.250789 1 TCCAACG 85 5.4158587E-5 11.155237 18 >>END_MODULE