FastQCFastQC Report
Thu 26 May 2016
SRR1926918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926918_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663711
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA18240.2748184074092489No Hit
GTCCTACAGTGGACATTTCTAAATT18210.2743664034496942No Hit
GTCCTAAAGTGTGTATTTCTCATTT14680.22118060420875954No Hit
GTCCTACAGTGTGCATTTCTCATTT14570.219523256357059No Hit
CTTTAGGACGTGAAATATGGCGAGG14540.21907125239750436No Hit
GTATCAACGCAGAGTACTTTTTTTT11720.17658288019936386No Hit
CTGTAGGACCTGGAATATGGCGAGA11230.16920014885997067No Hit
GGTATCAACGCAGAGTACTTTTTTT7250.10923429022571571No Hit
CTGAAGGACCTGGAATATGGCGAGA6710.10109821895373138No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTAGA456.598268E-414.823622
TAATACT759.4373354E-713.96071154
GCCTTAG551.9079346E-413.8599981
ACTGTTC2300.013.6170838
CTAATAC500.001475124213.3271413
TCCAACG655.5013614E-513.13898118
ATAGAGC604.016831E-412.6925153
AATCCCG1352.7284841E-1112.65235219
GGTCTAC1251.3387762E-912.1967981
TATACCT550.003020509112.1146674
CTACACT802.8036446E-511.8983344
ATACACT3000.011.42239954
TGGCGAG9400.011.40730718
GGCGAGG4800.011.26850219
GATTAAG1104.7614049E-711.2612491
GTTTAGA855.1508905E-511.2102931
TTAATAC855.202751E-511.1992793
GTAATAC600.00578121911.1059513
TAGACTT600.00578447311.1051125
ATTTCTC11800.011.09761415