FastQCFastQC Report
Thu 26 May 2016
SRR1926917_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926917_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685145
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15610.22783498383553846No Hit
CTGTAGGACGTGGAATATGGCAAGA13070.19076253931649506No Hit
GTCCTAAAGTGTGTATTTCTCATTT11580.16901531792540267No Hit
GTCCTACAGTGTGCATTTCTCATTT9880.14420305190872004No Hit
CTTTAGGACGTGAAATATGGCGAGG8230.12012055842193989No Hit
GTATCAACGCAGAGTACTTTTTTTT8150.11895292237409599No Hit
CTGTAGGACCTGGAATATGGCGAGA7460.10888206146144248No Hit
GTGTATATCAATGAGTTACAATGAA7140.10421151727006692No Hit
GTACATGGGAAGCAGTGGTATCAAC6860.10012479110261333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACT456.6320307E-414.8137594
CCAACGA1006.7666406E-1014.22213619
TCCAACG1301.8189894E-1213.85746618
TAGACTG852.631732E-713.4365395
CTAGACA1207.0940587E-1012.6975074
CCGTTTC751.5057223E-512.64189713
GTCCTAA3350.012.5364851
CCCGTTT701.1092656E-412.19040112
CGTTTCC701.1092656E-412.19040114
GACGTGG3700.012.0898587
CGAAATC550.003110699112.067265513
ACGAAAT550.003110699112.067265512
TAGGACC3000.012.0626324
TCACGTC1052.674733E-711.7741428
ATCCCGT752.1028593E-411.38103110
GTCCTAC9000.011.34773351
CGTGGAA3850.011.332599
ACGTGGA3950.011.31555758
GGCGAGG2600.011.30477419
GGACGTG6250.011.2687786
Produced by FastQC (version 0.11.5)