##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926917_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 685145 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.250465229987814 31.0 28.0 31.0 16.0 34.0 2 28.424940705981946 31.0 28.0 33.0 16.0 34.0 3 28.064668062964774 31.0 28.0 33.0 16.0 34.0 4 31.78849440629356 35.0 32.0 37.0 19.0 37.0 5 31.85318144334411 35.0 32.0 37.0 19.0 37.0 6 31.399286282465756 35.0 32.0 37.0 17.0 37.0 7 31.31677236205475 35.0 32.0 37.0 17.0 37.0 8 30.918154551226383 35.0 32.0 37.0 11.0 37.0 9 33.1464186413095 37.0 33.0 39.0 17.0 39.0 10 33.32569164191521 37.0 34.0 39.0 17.0 39.0 11 33.490900466324646 38.0 34.0 39.0 18.0 39.0 12 33.35838545125485 38.0 34.0 39.0 17.0 39.0 13 33.477644878091496 38.0 34.0 39.0 18.0 39.0 14 34.516185624940704 39.0 34.0 41.0 18.0 41.0 15 34.1071043355786 39.0 33.0 41.0 16.0 41.0 16 34.193356150887766 39.0 34.0 41.0 17.0 41.0 17 34.435563274927205 39.0 34.0 41.0 17.0 41.0 18 34.482019134635735 39.0 34.0 41.0 17.0 41.0 19 33.86034635004269 39.0 33.0 41.0 10.0 41.0 20 34.130084872545225 39.0 34.0 41.0 11.0 41.0 21 34.11048902057229 39.0 34.0 41.0 10.0 41.0 22 34.14051478154259 39.0 34.0 41.0 10.0 41.0 23 34.13429711958783 39.0 34.0 41.0 2.0 41.0 24 32.86218975545322 39.0 32.0 41.0 2.0 41.0 25 33.43031912952733 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.043574021154707765 1101 2 0.0753092044394883 1101 3 -0.8002046894093624 1101 4 -0.16550793926215945 1101 5 -0.05719290375051145 1101 6 -1.8367012010575046 1101 7 -0.19458178250607006 1101 8 0.12016648358925863 1101 9 2.6468227018483503E-4 1101 10 -8.871557446002399 1101 11 -3.58862913794502 1101 12 -1.0716087051048788 1101 13 -0.3536729020702012 1101 14 -0.06977706286389207 1101 15 -1.5553897492026394 1101 16 0.07066026900350408 1101 17 0.19811034710217257 1101 18 0.20220403056717373 1101 19 -0.505950912749185 1101 20 0.12353803664142049 1101 21 0.08548611015541496 1101 22 0.302819876525497 1101 23 0.11227279103616894 1101 24 0.09315256886871737 1101 25 0.06912831645698247 1102 1 0.23534839742469416 1102 2 0.03859869468618271 1102 3 -0.047652726060928075 1102 4 0.14103136067370414 1102 5 0.44566125921150146 1102 6 -0.431163305725466 1102 7 0.4863433778845021 1102 8 0.9107975962632615 1102 9 0.7364698942894421 1102 10 0.6725946667974796 1102 11 0.31154808589448635 1102 12 0.28649334146083305 1102 13 0.4042117605751301 1102 14 0.08359848184466756 1102 15 0.38613037978913667 1102 16 0.4047341995165752 1102 17 0.26931357109751985 1102 18 0.010189892620289243 1102 19 0.5770196145466855 1102 20 0.24909023775351358 1102 21 0.1826824969469456 1102 22 0.41824913983776213 1102 23 -0.1550205194132488 1102 24 -3.3440458558970683 1102 25 -1.7481033552613283 1103 1 -0.14523405261594746 1103 2 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0.28486370485778423 1104 14 0.22680904089379794 1104 15 0.634433883347981 1104 16 0.24467615238783225 1104 17 0.15934585240891153 1104 18 0.04645256574980294 1104 19 -0.4875578161768317 1104 20 -0.0844645565641855 1104 21 0.32646661691101286 1104 22 0.24540492924909785 1104 23 0.3684510542760009 1104 24 1.4269236693603418 1104 25 0.83881629685078 1105 1 0.04398680777084252 1105 2 0.1618047609974802 1105 3 0.6542082485299439 1105 4 0.2761450077565364 1105 5 0.09722760034718547 1105 6 -0.15728124766121 1105 7 0.446838816408043 1105 8 0.5837794870380861 1105 9 -0.40662224580246686 1105 10 0.02115634346493067 1105 11 0.08258680117326378 1105 12 0.1641911000904841 1105 13 -0.036481345136770926 1105 14 -0.23281532559730778 1105 15 0.5713711783892208 1105 16 0.038302970102897405 1105 17 -0.18350561193713588 1105 18 -0.0481429113161127 1105 19 -0.862065087037081 1105 20 -0.025249989290394126 1105 21 -0.007323836565419128 1105 22 0.1426778242960154 1105 23 0.0532586710522196 1105 24 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-1.1020090924054529 2215 1 0.28253180533640787 2215 2 -0.16097167893777709 2215 3 0.42193027555874707 2215 4 0.5437683414846397 2215 5 0.7981161690311431 2215 6 1.129065027653624 2215 7 0.6148452446316952 2215 8 -0.3016186746346996 2215 9 0.07459477418621674 2215 10 -0.2243876540085168 2215 11 -0.1223934272688254 2215 12 -0.4643593914021551 2215 13 -0.05159540169461252 2215 14 -0.04004263600747748 2215 15 0.08071990129791118 2215 16 0.1953256564029502 2215 17 -0.3909367240132511 2215 18 -1.1601301996189832 2215 19 0.19648800337914452 2215 20 -3.5270922642367672 2215 21 -1.888885296173541 2215 22 -1.0264158751236678 2215 23 -0.9356399775075204 2215 24 -0.8685829437714467 2215 25 -0.6828341181569826 2216 1 -0.3047669209313142 2216 2 -0.8667801498868393 2216 3 -0.09729587200193279 2216 4 -0.29711812124753223 2216 5 -0.6054970306346874 2216 6 -1.9133934301346827 2216 7 -9.825205367057347 2216 8 -10.693479462448984 2216 9 -5.868100148050882 2216 10 -3.3695321934392872 2216 11 -2.467344703862139 2216 12 -2.0522024293623495 2216 13 -1.9938676061784264 2216 14 -2.0532935143448725 2216 15 -3.068605323865455 2216 16 -2.0033116331680105 2216 17 -2.2753731289525376 2216 18 -2.0164691062462836 2216 19 -1.3839943097095144 2216 20 -2.2937406202867088 2216 21 -4.6454055923477675 2216 22 -2.88874077985124 2216 23 -3.0958821926343774 2216 24 -2.9681905529771093 2216 25 -2.4147716961201553 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39372.0 3 6.0 4 359.0 5 1835.0 6 2303.0 7 2117.0 8 1561.0 9 1365.0 10 1301.0 11 1250.0 12 1121.0 13 1150.0 14 1240.0 15 1356.0 16 1362.0 17 1529.0 18 1699.0 19 2077.0 20 2681.0 21 3471.0 22 4427.0 23 5062.0 24 6027.0 25 6882.0 26 8110.0 27 8769.0 28 9748.0 29 11486.0 30 13916.0 31 16652.0 32 21594.0 33 28308.0 34 38060.0 35 53548.0 36 77993.0 37 125031.0 38 180377.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.28963936756604 18.43234065206041 12.774306017657958 25.503713962715587 2 13.927099118952388 19.53429714203879 43.113317424108466 23.425286314900358 3 19.32667618522908 23.839030100383322 29.64588901862842 27.18840469575917 4 11.650013218567926 16.765964159423234 38.4685157081866 33.11550691382224 5 12.618561336558717 37.853056207234346 36.67580071330237 12.852581742904567 6 32.33648325306464 36.96749300355771 18.17965491517407 12.516368828203584 7 27.727047124604148 31.125927682831662 22.425291243728214 18.72173394883598 8 24.180804855660913 37.9765737198772 21.32213111514188 16.520490309320003 9 25.82392236093756 15.70883015871162 20.21018487159424 38.257062608756584 10 14.611664802642146 29.640179997605482 34.60252475551104 21.14563044424133 11 34.46577494128071 22.564554718782436 24.372189028653843 18.59748131128301 12 23.199635635331695 24.74789351676136 32.089783191344004 19.962687656562945 13 29.170449867600134 21.98173553308829 25.574632944357024 23.27318165495456 14 20.253504555228403 22.613523065177503 27.517637733436295 29.615334646157798 15 22.900305378277853 31.6105930644681 23.039710854066552 22.449390703187493 16 21.51481663691028 28.46189892949999 28.60875874261139 21.41452569097834 17 21.189967694704002 29.05053377458115 28.191005609997855 21.568492920716995 18 21.120855764851516 28.187659842813932 29.99045322371571 20.70103116861884 19 23.583573850953528 26.963813594829638 27.17798011915659 22.274632435060244 20 23.23123209043573 27.983078335685995 28.110662312656654 20.675027261221622 21 22.647411736884976 27.19712606597436 27.022395314821896 23.13306688231877 22 22.53860369789705 28.55826976021768 27.48679635290305 21.416330188982215 23 22.215182759219708 28.10035224888946 27.640224399914164 22.044240591976667 24 23.246648030421362 27.33353283360947 28.11654708814749 21.303272047821675 25 22.52602722129123 27.524479893497134 28.175233412937995 21.774259472273638 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 162.0 1 162.0 2 228.5 3 295.0 4 295.0 5 295.0 6 460.5 7 626.0 8 626.0 9 626.0 10 879.0 11 1132.0 12 1132.0 13 1132.0 14 1964.5 15 2797.0 16 2797.0 17 2797.0 18 4995.0 19 7193.0 20 7193.0 21 7193.0 22 11900.0 23 16607.0 24 16607.0 25 16607.0 26 24527.5 27 32448.0 28 32448.0 29 32448.0 30 43312.0 31 54176.0 32 54176.0 33 54176.0 34 61178.5 35 68181.0 36 68181.0 37 68181.0 38 73297.5 39 78414.0 40 78414.0 41 78414.0 42 82656.5 43 86899.0 44 86899.0 45 86899.0 46 89397.0 47 91895.0 48 91895.0 49 91895.0 50 87596.5 51 83298.0 52 83298.0 53 83298.0 54 73482.0 55 63666.0 56 63666.0 57 63666.0 58 54117.5 59 44569.0 60 44569.0 61 44569.0 62 36056.0 63 27543.0 64 27543.0 65 27543.0 66 21172.0 67 14801.0 68 14801.0 69 14801.0 70 10715.0 71 6629.0 72 6629.0 73 6629.0 74 4584.0 75 2539.0 76 2539.0 77 2539.0 78 1717.5 79 896.0 80 896.0 81 896.0 82 585.5 83 275.0 84 275.0 85 275.0 86 172.5 87 70.0 88 70.0 89 70.0 90 48.5 91 27.0 92 27.0 93 27.0 94 16.5 95 6.0 96 6.0 97 6.0 98 3.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2005414912171876 2 0.12347751205949106 3 0.12668851119106175 4 0.0732691620022039 5 0.023644629968838714 6 0.022476993920994826 7 0.310299279714513 8 0.30154200935568387 9 0.018828131271482678 10 0.03605076297718001 11 0.015471177633931505 12 0.01707667719971685 13 0.014449496092068102 14 0.015763086645892474 15 0.013573769056185188 16 0.020141721825307053 17 0.017222631705697336 18 0.014157587080107132 19 0.023936538980799685 20 0.015471177633931505 21 0.013281860044224216 22 0.015617132139911989 23 0.017222631705697336 24 0.01459545059804859 25 0.013573769056185188 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 685145.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.9334863320593 #Duplication Level Percentage of deduplicated Percentage of total 1 93.50267402082892 79.41508085959069 2 4.870402823404745 8.273205832665399 3 0.7853287756396942 2.0010213249590048 4 0.2855635891071096 0.9701564476944997 5 0.1520938500611898 0.6458930467681171 6 0.08479265985836505 0.4321041730283642 7 0.054531687651043735 0.3242096443441836 8 0.0394086752066443 0.2677692941622471 9 0.033290705528963474 0.25447461147259476 >10 0.15994251068474516 2.5145808669844536 >50 0.01650125748407817 0.9519990872911447 >100 0.013234969223558943 2.315483692724189 >500 0.0017188271699565781 1.0462815469458497 >1k 5.156481509869734E-4 0.5877395713693304 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1561 0.22783498383553846 No Hit CTGTAGGACGTGGAATATGGCAAGA 1307 0.19076253931649506 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1158 0.16901531792540267 No Hit GTCCTACAGTGTGCATTTCTCATTT 988 0.14420305190872004 No Hit CTTTAGGACGTGAAATATGGCGAGG 823 0.12012055842193989 No Hit GTATCAACGCAGAGTACTTTTTTTT 815 0.11895292237409599 No Hit CTGTAGGACCTGGAATATGGCGAGA 746 0.10888206146144248 No Hit GTGTATATCAATGAGTTACAATGAA 714 0.10421151727006692 No Hit GTACATGGGAAGCAGTGGTATCAAC 686 0.10012479110261333 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.459545059804859E-4 0.0 5 0.0 0.0 0.0 1.459545059804859E-4 0.0 6 0.0 0.0 0.0 1.459545059804859E-4 0.0 7 0.0 0.0 0.0 1.459545059804859E-4 0.0 8 0.0 0.0 0.0 1.459545059804859E-4 0.0 9 0.0 0.0 0.0 1.459545059804859E-4 0.0 10 0.0 0.0 0.0 2.919090119609718E-4 0.0 11 0.0 0.0 0.0 2.919090119609718E-4 0.0 12 0.0 0.0 0.0 2.919090119609718E-4 0.0 13 0.0 0.0 0.0 2.919090119609718E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGACT 45 6.6320307E-4 14.813759 4 CCAACGA 100 6.7666406E-10 14.222136 19 TCCAACG 130 1.8189894E-12 13.857466 18 TAGACTG 85 2.631732E-7 13.436539 5 CTAGACA 120 7.0940587E-10 12.697507 4 CCGTTTC 75 1.5057223E-5 12.641897 13 GTCCTAA 335 0.0 12.536485 1 CCCGTTT 70 1.1092656E-4 12.190401 12 CGTTTCC 70 1.1092656E-4 12.190401 14 GACGTGG 370 0.0 12.089858 7 CGAAATC 55 0.0031106991 12.0672655 13 ACGAAAT 55 0.0031106991 12.0672655 12 TAGGACC 300 0.0 12.062632 4 TCACGTC 105 2.674733E-7 11.774142 8 ATCCCGT 75 2.1028593E-4 11.381031 10 GTCCTAC 900 0.0 11.3477335 1 CGTGGAA 385 0.0 11.33259 9 ACGTGGA 395 0.0 11.3155575 8 GGCGAGG 260 0.0 11.304774 19 GGACGTG 625 0.0 11.268778 6 >>END_MODULE