FastQCFastQC Report
Thu 26 May 2016
SRR1926917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926917_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685145
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15430.22520780272788973No Hit
CTGTAGGACGTGGAATATGGCAAGA15390.22462398470396777No Hit
GTATCAACGCAGAGTACTTTTTTTT14130.20623371695042658No Hit
CTTTAGGACGTGAAATATGGCGAGG10810.15777682096490525No Hit
GTCCTACAGTGTGCATTTCTCATTT10520.15354414029147115No Hit
GTCCTAAAGTGTGTATTTCTCATTT10470.1528143677615687No Hit
GGTATCAACGCAGAGTACTTTTTTT8980.1310671463704763No Hit
CTGTAGGACCTGGAATATGGCGAGA8420.12289369403556913No Hit
GTACATGGGAAGCAGTGGTATCAAC8080.1179312408322326No Hit
TATCAACGCAGAGTACTTTTTTTTT7690.11223901509899364No Hit
GATATACACTGTTCTACAAATCCCG7310.10669274387173518No Hit
CCCATGTACTCTGCGTTGATACCAC6880.10041670011457428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1350.015.46054818
TAGGACC2850.013.3660344
CCAACGT957.494418E-812.98242119
GGCGAGG2850.012.64953919
GACCCTT550.003009689712.1205626
TATAGGT550.003009689712.1205624
ATAGGAC951.0058084E-612.0303113
AGGACCT6150.011.9234795
CTAGGAC802.7879281E-511.9049953
ACTGTTC3450.011.8254338
GTATTGG657.7590754E-411.7373221
TTGGACA657.8503205E-411.72098354
ATTAATG907.237182E-611.640443
AATCCCG1800.011.59541119
TAGGACA3000.011.4279584
GGACCTG5550.011.3250046
GTCCTAC7500.011.189581
TGGCGAG7750.011.13975518
CTAGACA600.005763880411.1105164
GATATAC3350.011.1022811
Produced by FastQC (version 0.11.5)