##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926917_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 685145 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.76231454655584 31.0 30.0 33.0 25.0 34.0 2 30.058689766399812 31.0 30.0 34.0 26.0 34.0 3 30.098986345955964 31.0 30.0 34.0 26.0 34.0 4 33.677136956410685 35.0 35.0 37.0 28.0 37.0 5 33.70594837589123 37.0 35.0 37.0 28.0 37.0 6 33.66374271139686 37.0 35.0 37.0 29.0 37.0 7 33.65415058126382 37.0 35.0 37.0 28.0 37.0 8 33.703478825650045 37.0 35.0 37.0 29.0 37.0 9 35.21430937976633 39.0 35.0 39.0 29.0 39.0 10 35.10197111560327 39.0 35.0 39.0 29.0 39.0 11 35.14423078326486 39.0 35.0 39.0 29.0 39.0 12 35.03925738347357 39.0 35.0 39.0 28.0 39.0 13 35.047848265695585 39.0 35.0 39.0 28.0 39.0 14 36.1434192762116 40.0 36.0 41.0 28.0 41.0 15 36.13605149274971 40.0 36.0 41.0 28.0 41.0 16 36.11385181239008 40.0 36.0 41.0 28.0 41.0 17 36.09328098431719 40.0 36.0 41.0 28.0 41.0 18 36.06338804194733 40.0 36.0 41.0 28.0 41.0 19 36.127241678768726 40.0 37.0 41.0 28.0 41.0 20 36.08913295725722 40.0 37.0 41.0 27.0 41.0 21 36.026740325040684 40.0 36.0 41.0 27.0 41.0 22 35.94091615643404 40.0 36.0 41.0 27.0 41.0 23 35.87633712571791 40.0 36.0 41.0 27.0 41.0 24 35.79976793233549 40.0 36.0 41.0 27.0 41.0 25 35.67817177385809 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.39700720511865484 1101 2 -0.3907222318568344 1101 3 -0.14622207318478075 1101 4 -0.3635483693897896 1101 5 -0.3475783429083492 1101 6 -0.317451756538226 1101 7 -0.2233210084534889 1101 8 -0.2486502905193504 1101 9 -0.04224784194102682 1101 10 -0.017063914416056036 1101 11 -0.15985493613067092 1101 12 -0.2208778718766169 1101 13 -0.1846895607417025 1101 14 -0.12467650738039993 1101 15 -0.2200088470478221 1101 16 -0.18376043974472367 1101 17 -0.17015679961620123 1101 18 -0.3145782896827498 1101 19 -0.31763531529163913 1101 20 -0.14158172341652175 1101 21 -0.17806019354449631 1101 22 -0.2203384785002669 1101 23 -0.0871850766560982 1101 24 -0.10084219727976773 1101 25 -0.2919311172906802 1102 1 -0.4975532623377781 1102 2 -0.32737519880768673 1102 3 -0.2858752579191517 1102 4 -0.17065210827334454 1102 5 -0.2215865787219542 1102 6 -0.26285202866773005 1102 7 -0.2614131448698913 1102 8 -0.12227390864293142 1102 9 -0.16880804110305547 1102 10 -0.3031125820404412 1102 11 -0.28126225725347354 1102 12 -0.1888007565292682 1102 13 -0.391941552262125 1102 14 -0.24804288120481743 1102 15 -0.26165045528923514 1102 16 -0.24038215775701843 1102 17 -0.35417788265966266 1102 18 -0.3570790439330267 1102 19 -0.2666499947003089 1102 20 -0.41147045401829985 1102 21 -0.27518165481199475 1102 22 -0.32921125895649084 1102 23 -0.11445596938872171 1102 24 -0.19460000202185057 1102 25 -0.043530027973460506 1103 1 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0.22886779819429393 2214 7 0.14996449372623033 2214 8 0.2350076380286268 2214 9 0.010179146755810109 2214 10 -0.22546636480042537 2214 11 -0.12176781460846087 2214 12 -0.16694459823169439 2214 13 -0.06386448195902972 2214 14 -0.18717871553553778 2214 15 -0.2846619060212916 2214 16 -0.1857820327013755 2214 17 -0.12684335188679086 2214 18 -0.3209696600832217 2214 19 -0.31902440644514485 2214 20 -0.293917283719189 2214 21 -0.13090520216599089 2214 22 -0.2091240664210332 2214 23 -0.3192822165243072 2214 24 -0.15324936643780518 2214 25 -0.2250789512305147 2215 1 0.6213008843116867 2215 2 0.4275387386562848 2215 3 0.5663960655497036 2215 4 0.46021407615030085 2215 5 0.5373422378152313 2215 6 0.5959151632503534 2215 7 0.48603380382201067 2215 8 0.3942608732496211 2215 9 0.24296928066376466 2215 10 0.4296200064241589 2215 11 0.40301211340565146 2215 12 0.36608469931392307 2215 13 0.43914356317422687 2215 14 0.16808732441189989 2215 15 0.2161424593070862 2215 16 0.3545783111259482 2215 17 0.39281438955600123 2215 18 0.3153188340146471 2215 19 0.25415359878804367 2215 20 0.40081164442516126 2215 21 0.4243355302430487 2215 22 0.5336137060419972 2215 23 0.38981798948793767 2215 24 0.2543115196516297 2215 25 0.2612867974582258 2216 1 -0.01874100122407185 2216 2 0.33780813360484174 2216 3 -1.6353561746398135 2216 4 -1.1443388300878965 2216 5 -0.4983749089329166 2216 6 -0.08088743113515307 2216 7 -0.2926451996226689 2216 8 -0.29576541965158754 2216 9 0.06812363344068473 2216 10 -0.11840816903732332 2216 11 0.029270253046995265 2216 12 0.1252350008668941 2216 13 -0.03055880347298512 2216 14 -0.19431554348845026 2216 15 -0.04485177857071676 2216 16 0.01734968018787697 2216 17 0.038145611446346095 2216 18 0.02705179083592668 2216 19 -0.06423011735162021 2216 20 0.05937014112353012 2216 21 0.060850476283953014 2216 22 -0.09150359634639216 2216 23 -0.20732340852764963 2216 24 -0.15019289206258435 2216 25 -0.14062480231746832 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17598.0 3 13.0 4 299.0 5 1939.0 6 2489.0 7 1875.0 8 1446.0 9 1235.0 10 1087.0 11 1005.0 12 953.0 13 922.0 14 868.0 15 917.0 16 898.0 17 1022.0 18 1120.0 19 1265.0 20 1672.0 21 2184.0 22 2641.0 23 3288.0 24 3943.0 25 4560.0 26 5484.0 27 6125.0 28 6985.0 29 8666.0 30 10885.0 31 14344.0 32 18335.0 33 24251.0 34 32441.0 35 45518.0 36 67402.0 37 115780.0 38 273690.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.707567612587795 19.46719425112182 10.685776991336645 28.13946114495373 2 14.021762315080222 20.520069073728315 40.73333859275427 24.724830018437192 3 19.12163148521211 24.20075326267846 29.04776502875828 27.629850223351145 4 12.199607382378913 17.274299600814427 37.612914054689156 32.913178962117506 5 12.959486916007892 38.56172421443501 35.35830637910298 13.120482490454116 6 32.12087395489633 37.90447653651657 17.363402443078556 12.611247065508541 7 28.337666622384884 31.603618649152494 21.097448639210985 18.961266089251644 8 24.830089859692762 38.992333607758646 19.3402698710201 16.83730666152849 9 25.95928293354392 16.04144983935925 19.59028944529675 38.40897778180008 10 14.914328001366197 30.007312662290747 33.71998487840692 21.358374457936137 11 34.63717240423001 22.787455937411053 24.002014289780398 18.573357368578538 12 23.290422395422834 24.944244971830575 31.66973173366027 20.095600899086318 13 29.22526782847301 22.110663514026328 25.414221910850337 23.24984674665032 14 20.52064967364525 22.937367910979297 26.942803264715153 29.599179150660298 15 22.72603871404676 31.76598173182743 22.920893383114933 22.58708617101088 16 21.663956750318913 28.607521404446988 28.160312229069177 21.568209616164918 17 21.23075036890109 29.263380053507078 27.83141621530104 21.67445336229079 18 21.15649862728184 28.259895466162245 29.863662304035543 20.71994360252037 19 23.361614616553382 27.207392346976754 27.12624116787639 22.304751868593474 20 23.154785101373886 28.078763809281938 27.972507688243375 20.7939434011008 21 22.543770272124533 27.111147442391403 27.052319963097798 23.292762322386267 22 22.632143560852082 28.718738369250307 27.01807646556568 21.63104160433193 23 22.02208586808029 28.303247200588487 27.526622140747055 22.14804479058417 24 22.91339789387648 27.258463536915546 28.328456020258486 21.49968254894949 25 22.552620055466356 27.692453656400524 27.908772441979274 21.846153846153847 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 235.0 1 235.0 2 256.5 3 278.0 4 278.0 5 278.0 6 424.5 7 571.0 8 571.0 9 571.0 10 860.0 11 1149.0 12 1149.0 13 1149.0 14 1926.5 15 2704.0 16 2704.0 17 2704.0 18 4913.0 19 7122.0 20 7122.0 21 7122.0 22 11510.5 23 15899.0 24 15899.0 25 15899.0 26 23423.5 27 30948.0 28 30948.0 29 30948.0 30 41519.0 31 52090.0 32 52090.0 33 52090.0 34 58981.0 35 65872.0 36 65872.0 37 65872.0 38 71399.0 39 76926.0 40 76926.0 41 76926.0 42 81721.0 43 86516.0 44 86516.0 45 86516.0 46 89718.5 47 92921.0 48 92921.0 49 92921.0 50 89179.5 51 85438.0 52 85438.0 53 85438.0 54 75247.5 55 65057.0 56 65057.0 57 65057.0 58 55544.0 59 46031.0 60 46031.0 61 46031.0 62 37510.5 63 28990.0 64 28990.0 65 28990.0 66 22230.5 67 15471.0 68 15471.0 69 15471.0 70 11187.5 71 6904.0 72 6904.0 73 6904.0 74 4790.5 75 2677.0 76 2677.0 77 2677.0 78 1826.0 79 975.0 80 975.0 81 975.0 82 619.5 83 264.0 84 264.0 85 264.0 86 178.5 87 93.0 88 93.0 89 93.0 90 52.5 91 12.0 92 12.0 93 12.0 94 7.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11209306059301316 2 0.09618401944114019 3 0.018244313247560734 4 0.0 5 0.004816498697356035 6 0.3383225448627663 7 0.020141721825307053 8 0.011238496960497413 9 0.011092542454516926 10 0.004670544191375548 11 0.005838180239219435 12 7.297725299024294E-4 13 7.297725299024294E-4 14 1.4595450598048587E-4 15 0.0027731356136292316 16 0.0010216815418634013 17 2.9190901196097174E-4 18 0.001167636047843887 19 5.838180239219435E-4 20 0.001167636047843887 21 0.013281860044224216 22 0.0 23 1.4595450598048587E-4 24 0.0 25 0.006567952769121865 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 685145.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.22136346269673 #Duplication Level Percentage of deduplicated Percentage of total 1 91.83418858521446 75.50732197966752 2 6.137515898232107 10.092698508532434 3 1.0384697392339322 2.5615319362369506 4 0.3422208191868299 1.1255144943544864 5 0.17221035621270753 0.707968514510275 6 0.09796383484784381 0.4832832042734497 7 0.06916582513841314 0.3980835913531956 8 0.05170495991235007 0.34010018414219995 9 0.035071281531692276 0.25952477273278507 >10 0.1848621997326054 2.7615846571098746 >50 0.01849133837971206 1.036339139744097 >100 0.015290431029680572 2.5655652912225606 >500 0.0017779570922652646 1.0385057439851912 >1k 0.0010667742553591587 1.1219779821349019 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1543 0.22520780272788973 No Hit CTGTAGGACGTGGAATATGGCAAGA 1539 0.22462398470396777 No Hit GTATCAACGCAGAGTACTTTTTTTT 1413 0.20623371695042658 No Hit CTTTAGGACGTGAAATATGGCGAGG 1081 0.15777682096490525 No Hit GTCCTACAGTGTGCATTTCTCATTT 1052 0.15354414029147115 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1047 0.1528143677615687 No Hit GGTATCAACGCAGAGTACTTTTTTT 898 0.1310671463704763 No Hit CTGTAGGACCTGGAATATGGCGAGA 842 0.12289369403556913 No Hit GTACATGGGAAGCAGTGGTATCAAC 808 0.1179312408322326 No Hit TATCAACGCAGAGTACTTTTTTTTT 769 0.11223901509899364 No Hit GATATACACTGTTCTACAAATCCCG 731 0.10669274387173518 No Hit CCCATGTACTCTGCGTTGATACCAC 688 0.10041670011457428 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 1.459545059804859E-4 0.0 12 0.0 0.0 0.0 1.459545059804859E-4 0.0 13 0.0 1.459545059804859E-4 0.0 1.459545059804859E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 135 0.0 15.460548 18 TAGGACC 285 0.0 13.366034 4 CCAACGT 95 7.494418E-8 12.982421 19 GGCGAGG 285 0.0 12.649539 19 GACCCTT 55 0.0030096897 12.120562 6 TATAGGT 55 0.0030096897 12.120562 4 ATAGGAC 95 1.0058084E-6 12.030311 3 AGGACCT 615 0.0 11.923479 5 CTAGGAC 80 2.7879281E-5 11.904995 3 ACTGTTC 345 0.0 11.825433 8 GTATTGG 65 7.7590754E-4 11.737322 1 TTGGACA 65 7.8503205E-4 11.7209835 4 ATTAATG 90 7.237182E-6 11.64044 3 AATCCCG 180 0.0 11.595411 19 TAGGACA 300 0.0 11.427958 4 GGACCTG 555 0.0 11.325004 6 GTCCTAC 750 0.0 11.18958 1 TGGCGAG 775 0.0 11.139755 18 CTAGACA 60 0.0057638804 11.110516 4 GATATAC 335 0.0 11.102281 1 >>END_MODULE