FastQCFastQC Report
Thu 26 May 2016
SRR1926916_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926916_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596656
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18550.31089941272693145No Hit
CTGTAGGACGTGGAATATGGCAAGA15800.26480920329301977No Hit
GTCCTAAAGTGTGTATTTCTCATTT15260.2557587621678153No Hit
CTTTAGGACGTGAAATATGGCGAGG12630.21167976187283793No Hit
GTACATGGGAAGCAGTGGTATCAAC12330.20665173902550213No Hit
GTCCTACAGTGTGCATTTCTCATTT10490.17581319889517577No Hit
GTATCAACGCAGAGTACTTTTTTTT10040.16827116462417205No Hit
CCCATGTACTCTGCGTTGATACCAC9040.15151108846638597No Hit
CTGTAGGACCTGGAATATGGCGAGA7220.12100774985921536No Hit
CTGAAGGACCTGGAATATGGCGAGA6810.11413611863452307No Hit
GTCCTTCAGTGTGCATTTCTCATTT6340.10625888284036362No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC350.002154420616.3011348
ACCGTGC350.002154420616.3011348
GCCCTAT456.544609E-414.839171
CAGTACT602.5121753E-514.2802984
ATAGCAC551.8974359E-413.8685713
TAGGACC2900.013.459594
AATCCCG957.526069E-812.977419
GTATTAA905.121983E-712.7192891
TAACACT751.4430871E-512.6935984
TATACTG853.857598E-612.3150745
GTGCTAC550.002970897112.1411391
GTCCTAA4250.012.1207351
TACTGGC550.003025770212.111527
TGTAGGA8750.011.7684742
CTGTAGG8600.011.6470241
GTAGGAC9050.011.5890683
GGACATG2550.011.56870656
AATGTCC1406.657501E-1011.5466368
TAGGACA3050.011.2369564
GTCTTAG855.0901835E-511.2229021
Produced by FastQC (version 0.11.5)