##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926916_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 596656 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.258928092569253 31.0 28.0 33.0 16.0 34.0 2 28.455726582821594 31.0 29.0 33.0 16.0 34.0 3 28.09768777989327 31.0 28.0 33.0 16.0 34.0 4 31.780310597731358 35.0 32.0 37.0 19.0 37.0 5 31.86453836046231 35.0 32.0 37.0 19.0 37.0 6 31.40640000268161 35.0 32.0 37.0 17.0 37.0 7 31.335994274757983 35.0 32.0 37.0 17.0 37.0 8 30.954268456195866 35.0 32.0 37.0 11.0 37.0 9 33.118051272424985 37.0 33.0 39.0 17.0 39.0 10 33.28556823362205 38.0 34.0 39.0 17.0 39.0 11 33.45675397549007 38.0 34.0 39.0 17.0 39.0 12 33.32401249631278 38.0 34.0 39.0 17.0 39.0 13 33.43103563862594 38.0 34.0 39.0 17.0 39.0 14 34.46542899090934 39.0 34.0 41.0 17.0 41.0 15 34.051902603845434 39.0 34.0 41.0 11.0 41.0 16 34.144046150545705 39.0 34.0 41.0 16.0 41.0 17 34.37382847067657 39.0 34.0 41.0 17.0 41.0 18 34.43857767289695 39.0 34.0 41.0 17.0 41.0 19 33.81621068086133 39.0 33.0 41.0 10.0 41.0 20 34.0862507039232 39.0 34.0 41.0 10.0 41.0 21 34.06312850285592 39.0 34.0 41.0 10.0 41.0 22 34.09542181759674 39.0 34.0 41.0 8.0 41.0 23 34.090576479579525 39.0 34.0 41.0 2.0 41.0 24 32.85698627014561 39.0 32.0 41.0 2.0 41.0 25 33.424851170523716 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.036309822888696885 1101 2 -0.10134753322786594 1101 3 -0.7434625230070004 1101 4 -0.2891688026368193 1101 5 -0.3051707752497741 1101 6 -2.063112322866502 1101 7 -0.4699796895884987 1101 8 -0.10920024374213355 1101 9 -0.4674913149612081 1101 10 -8.810934934072232 1101 11 -4.057705548385165 1101 12 -1.6754885833643378 1101 13 -0.9741449420576487 1101 14 -1.023830512269548 1101 15 -2.087376097872852 1101 16 -0.6392129319164326 1101 17 -0.4383754029773428 1101 18 -0.3516269576772686 1101 19 -0.8927030188699234 1101 20 -0.15935563347621695 1101 21 -0.2600669671719942 1101 22 -0.2343885424546528 1101 23 -0.4886245332138017 1101 24 -0.4887450138617595 1101 25 -0.12505187899066783 1102 1 -0.2912823366318271 1102 2 -0.3110216435411317 1102 3 -0.6157974081959843 1102 4 -0.5292540231844427 1102 5 -0.2456479051059013 1102 6 -0.9145734344159919 1102 7 -0.43035213494476565 1102 8 -0.08436266253273672 1102 9 -0.08027176684053217 1102 10 -0.019985671861235232 1102 11 -0.12461682958667097 1102 12 -0.1498934861760901 1102 13 -0.2680269484228859 1102 14 -0.6585894485908597 1102 15 -0.05167552710560841 1102 16 0.038101042675215524 1102 17 -0.06169795222903218 1102 18 -0.14605605891612328 1102 19 0.09738877733723683 1102 20 0.0526620418966246 1102 21 -0.07051700531081906 1102 22 -0.13896520149355496 1102 23 -0.5807679351970236 1102 24 -3.775293844709495 1102 25 -2.0840855200648676 1103 1 0.2918784639202805 1103 2 0.050845757881681664 1103 3 0.4149705053767683 1103 4 0.16944406779871102 1103 5 0.02370560288768786 1103 6 0.7129295881507751 1103 7 0.38591991163130146 1103 8 0.4793758763610789 1103 9 0.32081895167418395 1103 10 0.22293010619674902 1103 11 -0.08541686279598792 1103 12 -0.14134987040789326 1103 13 0.0830448304030611 1103 14 -0.08997329122021114 1103 15 0.24211017939667556 1103 16 0.24277940524831365 1103 17 -0.012470328303791689 1103 18 -0.16227673482950422 1103 19 -1.3405253661996568 1103 20 -0.4429039953497025 1103 21 -0.17956810095884634 1103 22 -0.11511416603741509 1103 23 -0.07433881892808358 1103 24 1.3544170785614185 1103 25 0.7427642129633512 1104 1 -0.21257680303498816 1104 2 -0.217413363948868 1104 3 -1.5872713004364662 1104 4 -0.598925855928357 1104 5 -0.5096509215402669 1104 6 -1.3732742873388126 1104 7 -0.07954081811514513 1104 8 0.01553852323801408 1104 9 -0.16683823241157114 1104 10 -0.020339322786966818 1104 11 -0.10509321818663153 1104 12 -0.19432348409578992 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-0.09785183510443218 2215 3 0.4553925681114883 2215 4 0.825884378041998 2215 5 0.5011657421496771 2215 6 1.370532642470085 2215 7 0.8526881513436848 2215 8 -0.4822869813163706 2215 9 0.26326089243215023 2215 10 0.07483838751844729 2215 11 0.22542115938079377 2215 12 -0.26910916010936603 2215 13 0.13532821920062332 2215 14 0.26218669523671423 2215 15 0.10268780926984533 2215 16 0.32835024131031076 2215 17 -0.22521685455017604 2215 18 -0.6406724153020917 2215 19 0.364260149840419 2215 20 -3.4381104603849586 2215 21 -1.7741468623913832 2215 22 -0.891755929488049 2215 23 -1.093470636684657 2215 24 -0.6473069902409563 2215 25 -0.41506158926323167 2216 1 -0.6242862892009953 2216 2 -1.1710694904310834 2216 3 -0.5482396798831068 2216 4 -0.677631389525196 2216 5 -0.622546178457263 2216 6 -1.9927406905008667 2216 7 -10.044560580960994 2216 8 -10.709856733678983 2216 9 -5.6791900110677 2216 10 -3.3976120500596956 2216 11 -2.1877019138388043 2216 12 -2.0543037212499407 2216 13 -1.8069285502535521 2216 14 -1.8481047387926495 2216 15 -2.9251413061777924 2216 16 -2.071010669411322 2216 17 -2.3547081456968897 2216 18 -2.0223302423985388 2216 19 -1.6386732155897406 2216 20 -2.2732168846687983 2216 21 -5.123044569280033 2216 22 -3.3695664080673 2216 23 -2.923341265756619 2216 24 -3.8042100086370993 2216 25 -3.173902453703313 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35501.0 3 4.0 4 278.0 5 1671.0 6 2204.0 7 1752.0 8 1470.0 9 1236.0 10 1206.0 11 1162.0 12 1091.0 13 1029.0 14 1116.0 15 1048.0 16 1168.0 17 1266.0 18 1433.0 19 1811.0 20 2384.0 21 3017.0 22 3716.0 23 4414.0 24 5217.0 25 5807.0 26 6798.0 27 7402.0 28 8210.0 29 9518.0 30 11612.0 31 14153.0 32 17946.0 33 23801.0 34 32407.0 35 45326.0 36 66462.0 37 108730.0 38 163290.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.687295431785955 18.981409916055622 12.761192023603895 25.57010262855453 2 13.612148026801659 19.978045734695797 43.590798007331514 22.81900823117103 3 18.68562930536719 25.003357011454124 29.932456929543044 26.378556753635646 4 11.433281955208786 17.24074313229895 39.11759328987158 32.208381622620685 5 12.038671541681545 38.501217915512036 36.94852232398061 12.511588218825805 6 31.13261002219555 37.64023094099739 18.809889424592132 12.417269612214927 7 27.072707405228208 31.43464739009834 23.537194250651424 17.955450954022023 8 23.846963339519622 38.36725705371312 21.676046961994675 16.10973264477259 9 26.10060784464926 15.585867695722289 20.705777038693522 37.607747420934935 10 14.569525320116764 29.663845961674852 35.11702265003194 20.649606068176446 11 33.58449639431539 22.52672833152854 25.355120541506444 18.533654732649634 12 23.520310320781032 25.0497867718056 32.36056351249866 19.069339394914707 13 29.07107727277299 22.701915308048452 25.72121590044287 22.505791518735688 14 19.828915694965033 23.512106318351584 27.821566747353106 28.83741123933028 15 22.596623681832888 32.90225573992527 22.959030586673414 21.542089991568425 16 20.509850333932356 28.56362148968699 29.88761633988681 21.038911836493845 17 20.735309401915718 29.760719350653268 28.720383278181778 20.783587969249233 18 20.60375549394367 27.917279898619768 31.277343998819912 20.20162060861665 19 23.498555763617983 27.167905262116747 27.67334608530634 21.660192888958925 20 23.34982834459822 28.07018426134535 28.589006007939062 19.990981386117372 21 22.00661787631209 27.359252790135542 27.407864343593165 23.2262649899592 22 22.422726640304013 29.40502991362013 27.467559353684766 20.704684092391094 23 21.12595781346127 29.002532935707688 28.478176688609835 21.393332562221204 24 23.036254282859844 27.405944710039492 29.01314863116112 20.54465237593955 25 21.734021492975643 28.178058793060963 28.73373820543208 21.354181508531315 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 235.0 1 235.0 2 293.5 3 352.0 4 352.0 5 352.0 6 528.0 7 704.0 8 704.0 9 704.0 10 1006.0 11 1308.0 12 1308.0 13 1308.0 14 2134.0 15 2960.0 16 2960.0 17 2960.0 18 5115.0 19 7270.0 20 7270.0 21 7270.0 22 11697.0 23 16124.0 24 16124.0 25 16124.0 26 23780.0 27 31436.0 28 31436.0 29 31436.0 30 41542.0 31 51648.0 32 51648.0 33 51648.0 34 58016.0 35 64384.0 36 64384.0 37 64384.0 38 67977.0 39 71570.0 40 71570.0 41 71570.0 42 73862.5 43 76155.0 44 76155.0 45 76155.0 46 78048.5 47 79942.0 48 79942.0 49 79942.0 50 74907.0 51 69872.0 52 69872.0 53 69872.0 54 59680.5 55 49489.0 56 49489.0 57 49489.0 58 41752.5 59 34016.0 60 34016.0 61 34016.0 62 27327.5 63 20639.0 64 20639.0 65 20639.0 66 15746.5 67 10854.0 68 10854.0 69 10854.0 70 7884.5 71 4915.0 72 4915.0 73 4915.0 74 3399.0 75 1883.0 76 1883.0 77 1883.0 78 1246.5 79 610.0 80 610.0 81 610.0 82 412.5 83 215.0 84 215.0 85 215.0 86 133.5 87 52.0 88 52.0 89 52.0 90 36.0 91 20.0 92 20.0 93 20.0 94 10.5 95 1.0 96 1.0 97 1.0 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2514011423667909 2 0.14614786409589445 3 0.1488294762811402 4 0.07089512214743504 5 0.024302110428789787 6 0.023464106620900486 7 0.30268697540961625 8 0.3003405647475262 9 0.020447292912498995 10 0.03905097744764152 11 0.01642487463463034 12 0.018771285296720387 13 0.0157544715883189 14 0.018100882250408946 15 0.014916467780429595 16 0.02145289748196616 17 0.01977688986618755 18 0.015084068542007455 19 0.024637311951945507 20 0.018771285296720387 21 0.015084068542007455 22 0.01759807996567536 23 0.01927408758145397 24 0.015419270065163176 25 0.014916467780429595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 596656.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.74337337783203 #Duplication Level Percentage of deduplicated Percentage of total 1 93.35147955009556 77.24216327786608 2 4.902533613854781 8.113043386171167 3 0.827801130182725 2.054851739919017 4 0.2937411692157228 0.9722054096342997 5 0.14445017876839067 0.5976147538163769 6 0.10116864050694803 0.5022620757356437 7 0.06763889410059222 0.3917669188600259 8 0.04374170167038701 0.2895468762795657 9 0.034002767885555354 0.25321533471308366 >10 0.1912855543174793 2.9088091703524213 >50 0.022497168967964463 1.3728071672685833 >100 0.017024834602608948 3.028352926400522 >500 0.001216059614427997 0.678520098571139 >1k 0.001418736216832663 1.5948408644121437 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1855 0.31089941272693145 No Hit CTGTAGGACGTGGAATATGGCAAGA 1580 0.26480920329301977 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1526 0.2557587621678153 No Hit CTTTAGGACGTGAAATATGGCGAGG 1263 0.21167976187283793 No Hit GTACATGGGAAGCAGTGGTATCAAC 1233 0.20665173902550213 No Hit GTCCTACAGTGTGCATTTCTCATTT 1049 0.17581319889517577 No Hit GTATCAACGCAGAGTACTTTTTTTT 1004 0.16827116462417205 No Hit CCCATGTACTCTGCGTTGATACCAC 904 0.15151108846638597 No Hit CTGTAGGACCTGGAATATGGCGAGA 722 0.12100774985921536 No Hit CTGAAGGACCTGGAATATGGCGAGA 681 0.11413611863452307 No Hit GTCCTTCAGTGTGCATTTCTCATTT 634 0.10625888284036362 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 1.676007615778606E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGATC 35 0.0021544206 16.301134 8 ACCGTGC 35 0.0021544206 16.301134 8 GCCCTAT 45 6.544609E-4 14.83917 1 CAGTACT 60 2.5121753E-5 14.280298 4 ATAGCAC 55 1.8974359E-4 13.868571 3 TAGGACC 290 0.0 13.45959 4 AATCCCG 95 7.526069E-8 12.9774 19 GTATTAA 90 5.121983E-7 12.719289 1 TAACACT 75 1.4430871E-5 12.693598 4 TATACTG 85 3.857598E-6 12.315074 5 GTGCTAC 55 0.0029708971 12.141139 1 GTCCTAA 425 0.0 12.120735 1 TACTGGC 55 0.0030257702 12.11152 7 TGTAGGA 875 0.0 11.768474 2 CTGTAGG 860 0.0 11.647024 1 GTAGGAC 905 0.0 11.589068 3 GGACATG 255 0.0 11.5687065 6 AATGTCC 140 6.657501E-10 11.546636 8 TAGGACA 305 0.0 11.236956 4 GTCTTAG 85 5.0901835E-5 11.222902 1 >>END_MODULE