FastQCFastQC Report
Thu 26 May 2016
SRR1926916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926916_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596656
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18540.3107318119653536No Hit
CTGTAGGACGTGGAATATGGCAAGA18280.3063741921643292No Hit
GTATCAACGCAGAGTACTTTTTTTT15470.25927837816095034No Hit
CTTTAGGACGTGAAATATGGCGAGG14600.2446971119036765No Hit
GTCCTAAAGTGTGTATTTCTCATTT14590.24452951114209862No Hit
GTACATGGGAAGCAGTGGTATCAAC12420.20816014587970288No Hit
CCCATGTACTCTGCGTTGATACCAC11880.19910970475449838No Hit
GTCCTACAGTGTGCATTTCTCATTT11330.18989166286771608No Hit
GGTATCAACGCAGAGTACTTTTTTT9340.1565391113137218No Hit
CTGTAGGACCTGGAATATGGCGAGA8800.14748867018851733No Hit
TATCAACGCAGAGTACTTTTTTTTT8500.14246064734118152No Hit
CTGAAGGACCTGGAATATGGCGAGA8440.14145504277171433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC704.320409E-714.9594243
CTAAAAC551.9229777E-413.8467393
TAGGACC3850.013.104954
TGTAGGA9900.012.4179452
CTGTAGG10000.012.1984641
TTAGACA550.003017594612.1158964
GACGTGG4650.012.0422247
GTAGGAC9350.012.0140833
GTCCTAA4150.011.9412671
GGCGAGG3500.011.93142219
GTCTTGG657.8011595E-411.7292931
GTCCTAC10750.011.6133641
CTATTAA752.0098721E-411.4360611
TGGACTG752.0304024E-411.423565
AGGACGT9500.011.2231465
ACGTGGA5200.011.1307628
CGTGGAA4950.011.1187939
GTGCTAT600.005732065511.1183911
TTCTTAG600.005732065511.1183912
AAGACCC600.005778938511.1062385
Produced by FastQC (version 0.11.5)