FastQCFastQC Report
Thu 26 May 2016
SRR1926915_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926915_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578454
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC8980.15524138479464228No Hit
GTATCAACGCAGAGTACTTTTTTTT8770.15161101833507937No Hit
GTCCTACAGTGGACATTTCTAAATT7680.13276768766401478No Hit
CCCATGTACTCTGCGTTGATACCAC6780.11720897426588804No Hit
GTCCTAAAGTGTGTATTTCTCATTT5850.10113163708782376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC601.4024536E-615.8975411
CCTACAC551.8949235E-413.8706013
GTTCAGG1259.458745E-1112.9723941
TAATACT550.003007952812.1209674
TAGGACC1459.276846E-1111.8224224
TGTAGAA2350.011.7678772
GTGTTGG907.1099385E-611.6581971
GATATAC2400.011.5257181
TCCAACG1001.9677846E-611.37795618
TTAGGAC2450.011.2875553
GGGCTAC600.005693881311.1282791
GTCCTAC3600.011.1282791
CTAGGAC600.00570495411.1253773
AGGACGT3550.010.993385
GGCGAGG2000.010.90387519
GTCTAGC700.001439492610.9011721
GTATAAA1158.33219E-710.782681
AGGACCT2600.010.6169655
GGACGTG3600.010.5762866
TATACAC3000.010.4896413
Produced by FastQC (version 0.11.5)