##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926915_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 578454 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.264081499998273 31.0 28.0 31.0 16.0 34.0 2 28.421264266475813 31.0 28.0 33.0 16.0 34.0 3 28.06210865513939 31.0 28.0 33.0 16.0 34.0 4 31.801802736259063 35.0 32.0 37.0 19.0 37.0 5 31.865330691809547 35.0 32.0 37.0 19.0 37.0 6 31.363354735207984 35.0 32.0 37.0 17.0 37.0 7 31.306124601091877 35.0 32.0 37.0 17.0 37.0 8 30.934513375307283 35.0 32.0 37.0 11.0 37.0 9 33.10419670362725 37.0 33.0 39.0 17.0 39.0 10 33.2976087986253 37.0 33.0 39.0 17.0 39.0 11 33.45894919907201 38.0 34.0 39.0 18.0 39.0 12 33.331421340331296 38.0 34.0 39.0 17.0 39.0 13 33.444858190971104 38.0 34.0 39.0 18.0 39.0 14 34.466341316682055 39.0 34.0 41.0 17.0 41.0 15 34.030908248538346 39.0 33.0 41.0 13.0 41.0 16 34.11977270448471 39.0 34.0 41.0 17.0 41.0 17 34.36177293267917 39.0 34.0 41.0 17.0 41.0 18 34.41635635677167 39.0 34.0 41.0 17.0 41.0 19 33.787870772784004 39.0 33.0 41.0 10.0 41.0 20 34.05684289502709 39.0 34.0 41.0 11.0 41.0 21 34.051480325142535 39.0 34.0 41.0 10.0 41.0 22 34.07858879011987 39.0 34.0 41.0 10.0 41.0 23 34.071350530897874 39.0 34.0 41.0 2.0 41.0 24 32.773817797093635 39.0 32.0 41.0 2.0 41.0 25 33.348871301780264 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.7319539882471595 1101 2 -1.0477809945858816 1101 3 -1.5217955564603471 1101 4 -0.9149402868408956 1101 5 -1.0172168900584246 1101 6 -2.5587594855423887 1101 7 -0.9974234378885178 1101 8 -0.654732930536646 1101 9 -1.115975233199471 1101 10 -9.53523904223259 1101 11 -4.565040221722125 1101 12 -2.266603839650152 1101 13 -1.6213626027829129 1101 14 -1.5152936911684023 1101 15 -2.845072834631928 1101 16 -1.270664329485193 1101 17 -1.2389223486928742 1101 18 -0.9768343600962837 1101 19 -1.8218048440696286 1101 20 -1.4868067101966815 1101 21 -1.2749906436665341 1101 22 -1.2181355827520122 1101 23 -1.539400136621694 1101 24 -1.377619308853479 1101 25 -1.1760469565028941 1102 1 0.014684420050123492 1102 2 0.061350269155653336 1102 3 -0.04295526213941514 1102 4 0.11272287673362058 1102 5 -0.028125288162108575 1102 6 -0.8947392596701249 1102 7 -0.03842588685448689 1102 8 0.5227454828226641 1102 9 0.41318498424534766 1102 10 0.35104327167172045 1102 11 0.2920130366820288 1102 12 0.1453926169283335 1102 13 -0.03927635078556335 1102 14 -0.25084569588554473 1102 15 0.3408205094929855 1102 16 0.27365694814911024 1102 17 0.11060670166181552 1102 18 -0.14899903185001762 1102 19 0.35964809781853546 1102 20 0.13634735215251226 1102 21 0.18921287221975547 1102 22 0.04455599911868546 1102 23 -0.24973238106215234 1102 24 -3.454185305079207 1102 25 -1.808001164594078 1103 1 0.29938192894691795 1103 2 0.0127144447191192 1103 3 0.42323690867909036 1103 4 0.4543598874097796 1103 5 -0.003214256133638571 1103 6 0.9237660784081676 1103 7 0.7231399494444481 1103 8 0.5096968469982279 1103 9 0.5306227066652767 1103 10 0.3735818244593858 1103 11 0.18287899160492316 1103 12 0.30909368454398845 1103 13 0.4957177180163299 1103 14 0.2805896540551416 1103 15 0.6148418618061555 1103 16 0.5844517286947806 1103 17 0.3383647087792525 1103 18 0.23059764667905114 1103 19 -1.3437919970806362 1103 20 0.014164671250973981 1103 21 0.03381548194692385 1103 22 0.07895694811038112 1103 23 0.3255938348334624 1103 24 1.7427304719077412 1103 25 1.2370759409812493 1104 1 -0.07175774875096863 1104 2 -0.05072113117197574 1104 3 -1.0721593518417265 1104 4 -0.23696765190962665 1104 5 -0.3579563902352021 1104 6 -1.6193379959617964 1104 7 -0.11982855468318476 1104 8 0.18701142644804136 1104 9 0.0776324951595555 1104 10 0.03211427658614241 1104 11 0.02165127394609101 1104 12 0.1996005397178493 1104 13 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1107 11 -0.24475308548338148 1107 12 0.09705567705460538 1107 13 -0.20903879733504027 1107 14 0.028134483418625678 1107 15 -1.3790396390991333 1107 16 -0.6843836443596487 1107 17 -0.4635717975125999 1107 18 -0.7365888134517462 1107 19 0.3821600830895022 1107 20 0.20157459076467887 1107 21 0.24427670732049478 1107 22 -0.08800793893118453 1107 23 -0.15951175111976568 1107 24 -3.977641101380744 1107 25 -1.5881974719749294 1108 1 0.5491308949948177 1108 2 0.0940750936242516 1108 3 0.6589946593122846 1108 4 0.7173793481516597 1108 5 0.7812799890011739 1108 6 -2.9740429049524195 1108 7 -1.1430791522194959 1108 8 0.2578908773274904 1108 9 0.3266292470134502 1108 10 0.4976483821202464 1108 11 0.3415863110239883 1108 12 0.517770546459289 1108 13 0.5693068943813131 1108 14 0.3349835497301754 1108 15 -0.6002060686866173 1108 16 0.25278169567243935 1108 17 0.13307600370407613 1108 18 0.11394476604040449 1108 19 0.15252296556884914 1108 20 -1.1284238959639268 1108 21 -0.2511308166648547 1108 22 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-0.07417220339989328 2215 5 -0.043785076063890926 2215 6 0.3991703082607927 2215 7 0.005429356289013754 2215 8 -1.4266736872370736 2215 9 -0.7628202024359965 2215 10 -1.0215107770389125 2215 11 -1.0521674427297683 2215 12 -1.292800024111287 2215 13 -0.6604028153283963 2215 14 -0.6023537876071643 2215 15 -0.628394256874806 2215 16 -0.6274185045277676 2215 17 -1.0018188911908226 2215 18 -1.8647539326582958 2215 19 -0.5089785359326555 2215 20 -4.805380852920887 2215 21 -2.942181867715906 2215 22 -1.9808537522697165 2215 23 -1.6979314698306354 2215 24 -1.256539260458073 2215 25 -1.2721770842860067 2216 1 -0.1532863846701673 2216 2 -0.9246107481808572 2216 3 0.009826959502383659 2216 4 -0.06508972642750877 2216 5 -0.20548806345495052 2216 6 -1.399986646859464 2216 7 -9.829194258151116 2216 8 -10.555306907613414 2216 9 -5.302570962370243 2216 10 -2.724984893357064 2216 11 -1.6481671121239323 2216 12 -1.2980070660155718 2216 13 -1.1018350732744224 2216 14 -1.148422792391763 2216 15 -1.9497247616915487 2216 16 -1.1681610819675896 2216 17 -1.4082987655606019 2216 18 -0.993423887016327 2216 19 -0.3911726566588811 2216 20 -1.426543299771403 2216 21 -3.978906665908596 2216 22 -2.499427371760767 2216 23 -2.5274031433390043 2216 24 -2.9240232582173 2216 25 -2.288137999329493 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33063.0 3 11.0 4 284.0 5 1552.0 6 2077.0 7 1686.0 8 1354.0 9 1213.0 10 1095.0 11 1078.0 12 1075.0 13 1046.0 14 1092.0 15 1110.0 16 1126.0 17 1245.0 18 1469.0 19 1792.0 20 2272.0 21 3001.0 22 3712.0 23 4503.0 24 5301.0 25 5886.0 26 6921.0 27 7429.0 28 8298.0 29 10113.0 30 11938.0 31 14276.0 32 18569.0 33 24071.0 34 32492.0 35 45472.0 36 64996.0 37 104928.0 38 150908.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.93223287111148 18.872788507515438 12.885733491334205 25.309245130038878 2 14.069069614873975 20.280695923712194 42.24338635328982 23.406848108124017 3 18.674017847096604 24.892978007045365 30.223391640773084 26.20961250508495 4 11.847691078119649 17.417508848531817 38.663153779352385 32.071646293996146 5 12.654478426191865 37.874902518860765 36.60189826686748 12.868720788079893 6 31.39644866050972 37.284395928184715 18.724851595232373 12.594303816073191 7 26.927425256024772 31.622917902140102 23.390069741391567 18.059587100443554 8 24.258707295800974 36.93814599231182 22.050217693232003 16.75292901865521 9 25.786472364102902 15.911346573480742 20.533525146020867 37.76865591639549 10 14.458306383832376 29.639666437235622 34.82521158961127 21.076815589320734 11 33.94103285865453 23.105837926813244 24.43529590771082 18.517833306821405 12 22.62962783652749 25.28650570244905 32.15347852849841 19.930387932525054 13 28.42124919599693 22.419236594761706 25.963939165496686 23.195575043744686 14 20.577265897462944 22.643326584213284 27.941529911973262 28.837877606350514 15 23.197795148704703 30.733227171265003 23.515762646513164 22.55321503351713 16 21.672312164015096 28.610481432106194 28.09849620549956 21.618710198379144 17 21.410453038788667 28.784156005387846 28.057073792360537 21.748317163462954 18 21.23413202020866 28.364737899530745 29.499832286130236 20.90129779413036 19 23.379542770387456 27.310659180472722 27.540812956395282 21.768985092744536 20 22.91743753782312 28.050488458545864 28.21457594881992 20.8174980548111 21 22.743541242336114 27.26423935182464 27.49419913479793 22.49802027104132 22 22.427750861940776 28.17292762463992 27.69656918177008 21.70275233164922 23 22.12402998907225 27.915911637364616 27.71153500338899 22.24852337017415 24 22.76057984245216 27.711441237698057 27.96284001078905 21.565138909060728 25 22.507261912995364 27.35787398851926 28.02251193028564 22.112352168199738 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 231.0 1 231.0 2 279.5 3 328.0 4 328.0 5 328.0 6 485.0 7 642.0 8 642.0 9 642.0 10 961.0 11 1280.0 12 1280.0 13 1280.0 14 2082.0 15 2884.0 16 2884.0 17 2884.0 18 5035.5 19 7187.0 20 7187.0 21 7187.0 22 11330.0 23 15473.0 24 15473.0 25 15473.0 26 22090.5 27 28708.0 28 28708.0 29 28708.0 30 37341.5 31 45975.0 32 45975.0 33 45975.0 34 51957.5 35 57940.0 36 57940.0 37 57940.0 38 61992.5 39 66045.0 40 66045.0 41 66045.0 42 69062.0 43 72079.0 44 72079.0 45 72079.0 46 74518.0 47 76957.0 48 76957.0 49 76957.0 50 73322.5 51 69688.0 52 69688.0 53 69688.0 54 61284.0 55 52880.0 56 52880.0 57 52880.0 58 44872.5 59 36865.0 60 36865.0 61 36865.0 62 29680.0 63 22495.0 64 22495.0 65 22495.0 66 17351.0 67 12207.0 68 12207.0 69 12207.0 70 8798.5 71 5390.0 72 5390.0 73 5390.0 74 3797.5 75 2205.0 76 2205.0 77 2205.0 78 1444.0 79 683.0 80 683.0 81 683.0 82 454.5 83 226.0 84 226.0 85 226.0 86 143.5 87 61.0 88 61.0 89 61.0 90 39.5 91 18.0 92 18.0 93 18.0 94 11.0 95 4.0 96 4.0 97 4.0 98 3.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21471024489414886 2 0.12913732120445187 3 0.1329405622573273 4 0.06776684057850754 5 0.023683819283815136 6 0.023683819283815136 7 0.3028762874835337 8 0.2982086734640957 9 0.019361954451002153 10 0.03664941378225408 11 0.016941710144626886 12 0.01832470689112704 13 0.016941710144626886 14 0.018497581484439557 15 0.01607733717806429 16 0.02022632741756475 17 0.01988057823093971 18 0.015212964211501692 19 0.025066816030315287 20 0.017978957704502 21 0.01590446258475177 22 0.018670456077752078 23 0.019016205264377115 24 0.01555871339812673 25 0.01625021177137681 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 578454.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.41725313741946 #Duplication Level Percentage of deduplicated Percentage of total 1 93.41551810733326 80.7271247524461 2 4.980138985892747 8.6073986280685 3 0.8132900371802431 2.108468730514391 4 0.2890455393519376 0.9991408616967333 5 0.1412585136677435 0.6103586366720509 6 0.08854682652338718 0.45911841133120307 7 0.04926007173846164 0.2979844062293057 8 0.033692744006458435 0.23293075101603125 9 0.026568522896000007 0.2066380891731862 >10 0.13787092822734143 2.190611287545672 >50 0.01240488851265392 0.7749488479401886 >100 0.010804210841481997 1.8486670419794478 >500 0.0016006238283070371 0.9366095553871715 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 898 0.15524138479464228 No Hit GTATCAACGCAGAGTACTTTTTTTT 877 0.15161101833507937 No Hit GTCCTACAGTGGACATTTCTAAATT 768 0.13276768766401478 No Hit CCCATGTACTCTGCGTTGATACCAC 678 0.11720897426588804 No Hit GTCCTAAAGTGTGTATTTCTCATTT 585 0.10113163708782376 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 1.7287459331251923E-4 0.0 13 0.0 0.0 0.0 1.7287459331251923E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAC 60 1.4024536E-6 15.897541 1 CCTACAC 55 1.8949235E-4 13.870601 3 GTTCAGG 125 9.458745E-11 12.972394 1 TAATACT 55 0.0030079528 12.120967 4 TAGGACC 145 9.276846E-11 11.822422 4 TGTAGAA 235 0.0 11.767877 2 GTGTTGG 90 7.1099385E-6 11.658197 1 GATATAC 240 0.0 11.525718 1 TCCAACG 100 1.9677846E-6 11.377956 18 TTAGGAC 245 0.0 11.287555 3 GGGCTAC 60 0.0056938813 11.128279 1 GTCCTAC 360 0.0 11.128279 1 CTAGGAC 60 0.005704954 11.125377 3 AGGACGT 355 0.0 10.99338 5 GGCGAGG 200 0.0 10.903875 19 GTCTAGC 70 0.0014394926 10.901172 1 GTATAAA 115 8.33219E-7 10.78268 1 AGGACCT 260 0.0 10.616965 5 GGACGTG 360 0.0 10.576286 6 TATACAC 300 0.0 10.489641 3 >>END_MODULE