FastQCFastQC Report
Thu 26 May 2016
SRR1926915_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926915_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578454
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14420.24928516355665273No Hit
GTACATGGGAAGCAGTGGTATCAAC9230.15956324962745524No Hit
GGTATCAACGCAGAGTACTTTTTTT8730.15091951996182928No Hit
CCCATGTACTCTGCGTTGATACCAC8130.14054704436307813No Hit
GTCCTACAGTGGACATTTCTAAATT7590.1312118163242021No Hit
TATCAACGCAGAGTACTTTTTTTTT7580.13103894173088956No Hit
CTGTAGGACGTGGAATATGGCAAGA7530.13017456876432698No Hit
GTCCTAAAGTGTGTATTTCTCATTT6250.10804662082032453No Hit
CTTTAGGACGTGAAATATGGCGAGG6040.10441625436076162No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT402.6818554E-416.6855951
TACACCC400.005189690314.2845615
ACCGTCT400.005325666614.2289948
TAGGACC1400.013.6043444
GGACCAC500.001470460713.3322576
TTATACA655.3186137E-513.1857494
TTCTAGG1001.3698082E-712.3950132
CCTGTCC853.9966053E-612.2759948
TAACACC550.003009320512.12023454
CTTACAC802.7890253E-511.9038013
GTTCTAG1052.5833106E-711.8047741
GTCCTAC3500.011.441551
CATGGAC1001.8672454E-611.42764953
GTATAGA855.115973E-511.2172061
TAATACT855.1797564E-511.2035774
CCTTGTA600.00571125411.1237292
TCCAACG600.005921443511.06986518
TGTAGAA3200.011.024412
AGGACGT3900.010.9881245
GTCTTAT700.00144441610.8967141