##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926915_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 578454 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.752644116904715 31.0 30.0 33.0 25.0 34.0 2 30.004029706770115 31.0 30.0 33.0 26.0 34.0 3 30.101263021778742 31.0 30.0 34.0 26.0 34.0 4 33.70645894055534 35.0 35.0 37.0 28.0 37.0 5 33.67005500869559 37.0 35.0 37.0 28.0 37.0 6 33.6389617843424 37.0 35.0 37.0 29.0 37.0 7 33.62353618438112 37.0 35.0 37.0 28.0 37.0 8 33.654430602952004 37.0 35.0 37.0 29.0 37.0 9 35.17987255684981 39.0 35.0 39.0 29.0 39.0 10 35.05177421195117 39.0 35.0 39.0 28.0 39.0 11 35.09801297942447 39.0 35.0 39.0 29.0 39.0 12 35.001336320606306 39.0 35.0 39.0 28.0 39.0 13 35.00957379497765 39.0 35.0 39.0 28.0 39.0 14 36.083386060084294 40.0 36.0 41.0 28.0 41.0 15 36.06051993762685 40.0 36.0 41.0 27.0 41.0 16 36.03862709912975 40.0 36.0 41.0 27.0 41.0 17 36.01975956601562 40.0 36.0 41.0 27.0 41.0 18 35.99117820950326 40.0 36.0 41.0 27.0 41.0 19 36.06240081320209 40.0 36.0 41.0 27.0 41.0 20 36.02259125185408 40.0 36.0 41.0 27.0 41.0 21 35.96575700055666 40.0 36.0 41.0 27.0 41.0 22 35.88253344259007 40.0 36.0 41.0 27.0 41.0 23 35.81132985509652 40.0 36.0 41.0 27.0 41.0 24 35.73570932174382 40.0 36.0 41.0 27.0 41.0 25 35.61384656342596 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2078292636547019 1101 2 -0.10045445882075299 1101 3 0.05116428968079134 1101 4 0.05266561636303635 1101 5 -0.152346288327891 1101 6 0.020093216542505843 1101 7 -0.029097698472746458 1101 8 -0.06619204026635828 1101 9 -0.08897094850318155 1101 10 -7.693910252726255E-5 1101 11 0.10974200223824226 1101 12 -0.0624719763761874 1101 13 0.13470748258334453 1101 14 0.09551374357712206 1101 15 -0.033572834843354826 1101 16 -0.333976450937854 1101 17 -0.2034696212845688 1101 18 -0.055083275312668434 1101 19 -0.1066691012680252 1101 20 -0.16717436066385005 1101 21 -0.0039027369854736094 1101 22 -0.11442249873942956 1101 23 -0.09794411311348483 1101 24 -0.051259881038944854 1101 25 0.14415835474009953 1102 1 -0.9855643942870778 1102 2 -0.8408028767275475 1102 3 -0.6932420968219439 1102 4 -0.8506662845824735 1102 5 -0.8414611143762158 1102 6 -0.9994581818434867 1102 7 -0.9007863446437483 1102 8 -0.6785392232601595 1102 9 -0.6108093000612129 1102 10 -0.7612533884217285 1102 11 -0.662452707939643 1102 12 -0.4948889987061449 1102 13 -0.7570012170171196 1102 14 -0.6485449759794264 1102 15 -0.5067155632501112 1102 16 -0.7239447298056803 1102 17 -0.772745041648669 1102 18 -0.5743182967776264 1102 19 -0.7645689982195449 1102 20 -0.8367795083597187 1102 21 -0.9104957538862593 1102 22 -0.8880788007590965 1102 23 -0.9173450412451629 1102 24 -0.7619439644289514 1102 25 -0.9382401021749232 1103 1 -0.6142713931008394 1103 2 -0.502724584912599 1103 3 -0.32906653335812663 1103 4 -0.33465204970703155 1103 5 -0.6362416600464371 1103 6 -0.6210477429348273 1103 7 -0.6730283375263113 1103 8 -0.44959497652232017 1103 9 -0.49455781726168624 1103 10 -0.3792842306518551 1103 11 -0.5094277969076089 1103 12 -0.6692662229054278 1103 13 -0.5565267211689573 1103 14 -0.4456980008904523 1103 15 -0.41537511247905456 1103 16 -0.27910487215443425 1103 17 -0.22826105588355006 1103 18 -0.4794191271453627 1103 19 -0.3292190575315246 1103 20 -0.2246798642316037 1103 21 -0.546320190422442 1103 22 -0.5369518731197118 1103 23 -0.6421374493115977 1103 24 -0.41437575565077367 1103 25 -0.2822495920918868 1104 1 -0.22434958807989958 1104 2 -0.2362483621577809 1104 3 0.01573378297243977 1104 4 -0.18171590490510425 1104 5 -0.06699593091255451 1104 6 0.09843483064027225 1104 7 -0.06524596004388172 1104 8 0.016807005385508944 1104 9 -0.02112716386153579 1104 10 -0.06238071954397384 1104 11 0.015733394496592723 1104 12 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10 0.2977428997962335 2214 11 0.37954639281419844 2214 12 0.17173461894203967 2214 13 0.328331504063506 2214 14 0.4882121892905431 2214 15 0.5138511053298487 2214 16 0.3768413931040513 2214 17 0.4793918637965504 2214 18 0.38034032450580924 2214 19 0.5147760827126149 2214 20 0.5621928159046163 2214 21 0.5349934264604315 2214 22 0.34169892280880276 2214 23 0.5989250961290367 2214 24 0.5900535100286319 2214 25 0.756275692031636 2215 1 0.2502372436497886 2215 2 0.21812437792924655 2215 3 0.14786591510457114 2215 4 -0.0998585373334464 2215 5 -0.15846495095300384 2215 6 0.09900683121227871 2215 7 -0.2603594408716532 2215 8 -0.23391310247745167 2215 9 -0.44624616040910325 2215 10 -0.18905841765024434 2215 11 -0.6047544402769631 2215 12 -0.44915643340062417 2215 13 -0.49975464660328583 2215 14 -0.28353940100943475 2215 15 -0.3649581474713415 2215 16 -0.27386475659403686 2215 17 -0.3408790119178704 2215 18 -0.33392131616712817 2215 19 -0.2278899704373103 2215 20 -0.3728137632551878 2215 21 -0.2643976985355323 2215 22 -0.3706717139569946 2215 23 -0.34396739313442026 2215 24 -0.3692202798903139 2215 25 -0.40644852412000176 2216 1 0.37960514365989084 2216 2 0.43701299131327076 2216 3 -1.2350307430214436 2216 4 -0.8442634927475794 2216 5 0.18610898077689342 2216 6 0.5527817070055008 2216 7 0.438862717066101 2216 8 0.6808732435015514 2216 9 0.47478496245688007 2216 10 0.554253701808662 2216 11 0.37568471447411866 2216 12 0.5896713852437117 2216 13 0.5174735908084358 2216 14 0.4191541181978451 2216 15 0.5323795572058145 2216 16 0.48469196526544067 2216 17 0.35444016046552207 2216 18 0.43538927883154344 2216 19 0.417085280042528 2216 20 0.43517771446106224 2216 21 0.531572583673281 2216 22 0.72356013256843 2216 23 0.4588996941363348 2216 24 0.45308826718887474 2216 25 0.43214236034244635 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14893.0 3 14.0 4 250.0 5 1610.0 6 2173.0 7 1627.0 8 1210.0 9 1082.0 10 924.0 11 890.0 12 864.0 13 760.0 14 754.0 15 767.0 16 702.0 17 810.0 18 877.0 19 1099.0 20 1440.0 21 1870.0 22 2256.0 23 2847.0 24 3288.0 25 3911.0 26 4826.0 27 5203.0 28 6071.0 29 7348.0 30 9531.0 31 12235.0 32 15740.0 33 21030.0 34 27850.0 35 39021.0 36 58006.0 37 98518.0 38 226157.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.53742308990368 19.852886454303935 10.743961854322976 27.865728601469403 2 14.2063783244957 21.256870509075302 39.77955530327154 24.75719586315746 3 18.289945984825955 25.285983027754533 29.7085186286647 26.715552358754813 4 12.229321605520923 17.91983459358912 37.7558111794542 32.09503262143576 5 13.050769512705346 38.57902070820756 35.164189219560946 13.206020559526156 6 31.18599261746829 38.19228580944187 17.84123393744276 12.78048763564708 7 27.495789484605247 32.26326874327788 21.88828214430223 18.352659627814646 8 24.96931136430436 37.916976434585656 20.082816092947663 17.030896108162313 9 26.04959637890762 16.094358640983835 20.061239062291985 37.794805917816554 10 14.716962212644425 30.185798925004452 33.76122236225863 21.336016500092494 11 33.91595395049627 23.36740885963684 24.021776526094314 18.69486066377258 12 22.703353087205162 25.594690657133174 31.60244929570647 20.09950695995519 13 28.575207148771973 22.440872056136325 25.86001531682136 23.12390547827034 14 20.864926165261195 22.738713882175592 27.435543707883426 28.960816244679783 15 22.927050216185286 30.843701755932777 23.577594180198776 22.65165384768316 16 21.87985132682168 28.83205117123347 27.65718731091711 21.63091019102775 17 21.30834949710781 29.159449152395865 27.764005435177214 21.768195915319108 18 21.219947757026954 28.52238132529808 29.279230220018636 20.97844069765633 19 23.09760153789238 27.513683024060686 27.58871059755832 21.80000484048861 20 22.958755009110618 28.047389038907696 28.095448840514063 20.89840711146762 21 22.619953249699165 27.294118867480876 27.412896443934216 22.673031438885737 22 22.41198090081493 28.201896780037828 27.461302022286993 21.92482029686025 23 22.231845574583286 27.863581200925225 27.690360858426082 22.214212366065407 24 22.576903262835074 27.61274016602876 28.153146144723696 21.657210426412473 25 22.460760228964215 27.590326244616904 27.86434337025505 22.084570156163828 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 231.0 1 231.0 2 265.5 3 300.0 4 300.0 5 300.0 6 425.0 7 550.0 8 550.0 9 550.0 10 894.0 11 1238.0 12 1238.0 13 1238.0 14 1998.5 15 2759.0 16 2759.0 17 2759.0 18 4870.0 19 6981.0 20 6981.0 21 6981.0 22 10943.0 23 14905.0 24 14905.0 25 14905.0 26 21197.0 27 27489.0 28 27489.0 29 27489.0 30 35967.0 31 44445.0 32 44445.0 33 44445.0 34 50307.5 35 56170.0 36 56170.0 37 56170.0 38 60589.5 39 65009.0 40 65009.0 41 65009.0 42 68342.0 43 71675.0 44 71675.0 45 71675.0 46 74740.5 47 77806.0 48 77806.0 49 77806.0 50 74450.0 51 71094.0 52 71094.0 53 71094.0 54 62820.5 55 54547.0 56 54547.0 57 54547.0 58 46111.5 59 37676.0 60 37676.0 61 37676.0 62 30682.0 63 23688.0 64 23688.0 65 23688.0 66 18223.0 67 12758.0 68 12758.0 69 12758.0 70 9283.0 71 5808.0 72 5808.0 73 5808.0 74 4028.5 75 2249.0 76 2249.0 77 2249.0 78 1510.0 79 771.0 80 771.0 81 771.0 82 490.5 83 210.0 84 210.0 85 210.0 86 143.0 87 76.0 88 76.0 89 76.0 90 46.0 91 16.0 92 16.0 93 16.0 94 9.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11565310292607536 2 0.10752799704038696 3 0.016941710144626886 4 0.0 5 0.0062234853592506925 6 0.3384884537059127 7 0.02420244306375269 8 0.01106397397200123 9 0.01123684856531375 10 0.005704861579313134 11 0.005704861579313134 12 3.4574918662503846E-4 13 8.643729665625962E-4 14 0.0 15 0.0025931188996877884 16 6.914983732500769E-4 17 0.0 18 5.186237799375577E-4 19 0.0 20 0.0013829967465001538 21 0.010718224785376191 22 0.0 23 0.0 24 0.0 25 0.003976115646187942 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 578454.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.67178675799761 #Duplication Level Percentage of deduplicated Percentage of total 1 91.76064113462269 76.77776797793292 2 6.183257498000256 10.347284056849345 3 1.0935534778320362 2.744987202168864 4 0.38068545350781674 1.2741052835111062 5 0.1635151970228126 0.684080434849237 6 0.10074029635672332 0.5057472356818342 7 0.06202049400211674 0.36325558841395467 8 0.03633515457683543 0.24321818444574833 9 0.03116870801153773 0.23471473412373087 >10 0.15998491672443194 2.4731061043002835 >50 0.01322245222601843 0.7963959060478438 >100 0.012396014264048138 1.9846758130014206 >500 0.002272602615013464 1.3213885475437126 >1k 2.0660023772849675E-4 0.24927293113004104 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1442 0.24928516355665273 No Hit GTACATGGGAAGCAGTGGTATCAAC 923 0.15956324962745524 No Hit GGTATCAACGCAGAGTACTTTTTTT 873 0.15091951996182928 No Hit CCCATGTACTCTGCGTTGATACCAC 813 0.14054704436307813 No Hit GTCCTACAGTGGACATTTCTAAATT 759 0.1312118163242021 No Hit TATCAACGCAGAGTACTTTTTTTTT 758 0.13103894173088956 No Hit CTGTAGGACGTGGAATATGGCAAGA 753 0.13017456876432698 No Hit GTCCTAAAGTGTGTATTTCTCATTT 625 0.10804662082032453 No Hit CTTTAGGACGTGAAATATGGCGAGG 604 0.10441625436076162 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAT 40 2.6818554E-4 16.685595 1 TACACCC 40 0.0051896903 14.284561 5 ACCGTCT 40 0.0053256666 14.228994 8 TAGGACC 140 0.0 13.604344 4 GGACCAC 50 0.0014704607 13.332257 6 TTATACA 65 5.3186137E-5 13.185749 4 TTCTAGG 100 1.3698082E-7 12.395013 2 CCTGTCC 85 3.9966053E-6 12.275994 8 TAACACC 55 0.0030093205 12.1202345 4 CTTACAC 80 2.7890253E-5 11.903801 3 GTTCTAG 105 2.5833106E-7 11.804774 1 GTCCTAC 350 0.0 11.44155 1 CATGGAC 100 1.8672454E-6 11.4276495 3 GTATAGA 85 5.115973E-5 11.217206 1 TAATACT 85 5.1797564E-5 11.203577 4 CCTTGTA 60 0.005711254 11.123729 2 TCCAACG 60 0.0059214435 11.069865 18 TGTAGAA 320 0.0 11.02441 2 AGGACGT 390 0.0 10.988124 5 GTCTTAT 70 0.001444416 10.896714 1 >>END_MODULE