FastQCFastQC Report
Thu 26 May 2016
SRR1926914_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926914_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859498
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10810.12577108963604336No Hit
GTCCTAAAGTGTGTATTTCTCATTT9300.10820269506153593No Hit
GTACATGGGAAGCAGTGGTATCAAC8700.1012218760253078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2150.014.6095934
CCTAGAC853.7983136E-612.3337433
TTGGGCC701.0703183E-412.2379064
GTCTAGC550.002972554412.1412411
GGACCTG4200.011.5553226
GAACCAT752.0393197E-411.4193776
GTGTTAG855.0958435E-511.2229971
GTAGGAC6200.011.2215363
AGATAGT600.005798241611.1021746
TGGGCCG600.005798241611.1021745
TGTAGGA6800.011.0723372
CTAGACA951.3293342E-511.0212734
GTCCTAA3050.010.9470211
GGCGAGG2350.010.89717219
TGTTAGG700.001450738310.8921372
GCTGATT700.001480027210.8660788
GACGTGA3450.010.7573847
TGGCGAG5650.010.74358918
TAGACTT803.7082922E-410.7056675
GTGTTAT909.110699E-510.5994971
Produced by FastQC (version 0.11.5)