##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926914_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 859498 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.99323093247454 31.0 28.0 31.0 16.0 34.0 2 28.188757856330092 31.0 28.0 31.0 16.0 34.0 3 27.656540213008057 31.0 28.0 31.0 16.0 34.0 4 31.416498933098158 35.0 32.0 37.0 18.0 37.0 5 31.29421708951039 35.0 32.0 37.0 17.0 37.0 6 31.044685386120737 35.0 32.0 37.0 13.0 37.0 7 31.16175372135828 35.0 32.0 37.0 16.0 37.0 8 30.752598609886235 35.0 31.0 37.0 11.0 37.0 9 32.97638621614012 37.0 33.0 39.0 17.0 39.0 10 33.15652741483982 37.0 33.0 39.0 17.0 39.0 11 33.31035674312215 38.0 33.0 39.0 17.0 39.0 12 33.18533958194202 38.0 33.0 39.0 17.0 39.0 13 33.28706873081729 38.0 34.0 39.0 17.0 39.0 14 34.29717695678175 39.0 34.0 41.0 17.0 41.0 15 33.84859534286293 39.0 33.0 41.0 11.0 41.0 16 33.92987650931125 39.0 33.0 40.0 16.0 41.0 17 34.153323218902194 39.0 34.0 41.0 17.0 41.0 18 34.20715813183975 39.0 34.0 41.0 17.0 41.0 19 33.57008160577453 39.0 33.0 40.0 10.0 41.0 20 33.84825677313967 39.0 34.0 41.0 10.0 41.0 21 33.835730856849 39.0 34.0 41.0 10.0 41.0 22 33.85535742956935 39.0 34.0 41.0 2.0 41.0 23 33.85377394711797 39.0 34.0 41.0 2.0 41.0 24 32.54262139062569 38.0 32.0 40.0 2.0 41.0 25 33.11772104181743 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.5237810234536191 1101 2 -0.6260476575529026 1101 3 -1.2870540105225352 1101 4 -0.6946752370270204 1101 5 -0.6639304800495687 1101 6 -2.16004077562717 1101 7 -0.5991156022861901 1101 8 -0.26052852136396965 1101 9 -0.3296697831256381 1101 10 -8.703318173873036 1101 11 -3.8553225962416384 1101 12 -1.4775101453836257 1101 13 -0.7301328723679887 1101 14 -0.7262094224533584 1101 15 -2.05240541318906 1101 16 -0.5891289570211811 1101 17 -0.44172851501787846 1101 18 -0.39775977617681946 1101 19 -1.0522505841272007 1101 20 -0.5807618416864813 1101 21 -0.5208052325858077 1101 22 -0.36059689424126873 1101 23 -0.49541805840001985 1101 24 -0.21569726481906315 1101 25 -0.15751686649013408 1102 1 -0.23263195513063906 1102 2 -0.4381594587951376 1102 3 -0.7459049421995552 1102 4 -0.7117559823686364 1102 5 -0.41625967259615493 1102 6 -1.152276800471892 1102 7 -0.06883609918060074 1102 8 0.3792230314310636 1102 9 0.13539232867560003 1102 10 0.18410418637540715 1102 11 -0.1930555154962974 1102 12 0.007738301821341054 1102 13 -0.19752417671580957 1102 14 -0.4404951367390737 1102 15 0.1098616675562809 1102 16 0.09254806161236218 1102 17 -0.2522875212290572 1102 18 -0.2890641240029126 1102 19 -0.17802698164272357 1102 20 -0.03573078578585864 1102 21 -0.10077417668518507 1102 22 -0.06867142840275875 1102 23 -0.5497658844869804 1102 24 -4.153585463576825 1102 25 -2.1978895372975877 1103 1 -0.1630547931621713 1103 2 -0.24081115492509397 1103 3 -0.1592995910289865 1103 4 -0.4939705069744633 1103 5 -0.5476643548704025 1103 6 0.3022931947502805 1103 7 0.38522983488533313 1103 8 0.33220439789068834 1103 9 0.32770479881416037 1103 10 0.26557575828176283 1103 11 0.1476109919093389 1103 12 -0.14638735513040046 1103 13 0.09241610039838122 1103 14 -0.09362461595551252 1103 15 0.45132129488547434 1103 16 0.2471801686357722 1103 17 0.012779368403045055 1103 18 -0.13261644125088168 1103 19 -1.940062337591126 1103 20 -0.5062515693501197 1103 21 -0.22509333578695845 1103 22 -0.11431165773863938 1103 23 -0.2779073082805752 1103 24 1.2855425822903541 1103 25 0.5999676055595558 1104 1 -0.34973072292034857 1104 2 -0.2269638185566194 1104 3 -1.5332716835022318 1104 4 -0.63383037807953 1104 5 -0.4783106033183415 1104 6 -1.5850059242705932 1104 7 -0.12944881467417702 1104 8 0.05462561234111263 1104 9 0.1698581719590777 1104 10 0.18524879086025692 1104 11 0.08341540271759129 1104 12 0.07718977488792689 1104 13 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-0.3852902166343597 2214 24 -1.7075940868657362 2214 25 -0.6231669298079936 2215 1 0.422264816805324 2215 2 0.06612275623416863 2215 3 0.2924447209970147 2215 4 0.5743935233613975 2215 5 0.654805850980086 2215 6 1.1120548415541798 2215 7 0.6439405607791571 2215 8 -0.6907533485881849 2215 9 -0.09977082415441885 2215 10 -0.4067102655535564 2215 11 -0.41169561697473256 2215 12 -0.6669073984673446 2215 13 -0.10305518887572873 2215 14 0.06953898101090061 2215 15 -0.1352984234243877 2215 16 0.0575051957213617 2215 17 -0.34914912563812095 2215 18 -1.0339923892465421 2215 19 0.282462039440297 2215 20 -3.6860457188625837 2215 21 -2.0555375394705955 2215 22 -1.0353300759401591 2215 23 -0.876119659309829 2215 24 -1.2172959875904716 2215 25 -1.0779197183841092 2216 1 0.0867583707731292 2216 2 -0.4953779621636585 2216 3 0.3557492532565014 2216 4 0.06742115479887545 2216 5 0.19024094593566332 2216 6 -1.1037010400225817 2216 7 -9.297744595264668 2216 8 -10.619447232921246 2216 9 -5.454767843047797 2216 10 -3.0017797106767574 2216 11 -1.8913224410698959 2216 12 -1.5737209375784893 2216 13 -1.2235407671615448 2216 14 -1.2823557321675452 2216 15 -2.2276169424497105 2216 16 -1.4372734693293197 2216 17 -1.61281236810472 2216 18 -1.3196884532083857 2216 19 -0.771355243158844 2216 20 -1.6971686675191293 2216 21 -4.037339105284634 2216 22 -2.7104041148997986 2216 23 -2.3786653288859227 2216 24 -2.5862382323148907 2216 25 -2.1952995700129634 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52310.0 3 14.0 4 385.0 5 2242.0 6 2687.0 7 2306.0 8 1861.0 9 1712.0 10 1646.0 11 1605.0 12 1656.0 13 1568.0 14 1617.0 15 1724.0 16 1752.0 17 1971.0 18 2248.0 19 2858.0 20 3449.0 21 4627.0 22 5858.0 23 6860.0 24 8045.0 25 9399.0 26 10527.0 27 11501.0 28 13047.0 29 15252.0 30 18412.0 31 22473.0 32 28664.0 33 37593.0 34 50207.0 35 69938.0 36 100599.0 37 157623.0 38 203262.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.696402542125824 18.71214506442773 12.99329485161215 25.598157541834293 2 14.361958065421856 19.88927425941093 41.920597620748026 23.828170054419186 3 18.23213882099092 25.276996530407285 29.389477510561402 27.10138713804039 4 12.14064426445369 17.106689345967613 38.421506683291945 32.331159706286755 5 12.990640175675628 37.713586815483566 35.825213455896645 13.470559552944161 6 32.129478671000314 36.87391120684539 18.50933816504388 12.487271957110423 7 27.875995187859033 31.138892810843423 23.296759972882118 17.688352028415437 8 24.970916019934634 36.28607201158459 20.94454965630145 17.798462312179332 9 26.412117093424314 15.429721137420463 20.178321238892742 37.97984053026248 10 14.597646434444785 29.167825681122245 34.682096398190424 21.552431486242547 11 34.04358562009668 22.638317084920743 24.527151411622057 18.790945883360525 12 23.100070866609027 24.878136196546038 31.724851372124167 20.296941564720765 13 28.55779737087388 22.16325157993095 25.675696117983694 23.603254931211477 14 21.045459729052286 22.024101825324603 27.657752218526937 29.272686227096166 15 23.298129577438566 30.597129551838652 23.148021010544838 22.956719860177945 16 22.38332852341808 27.963916326093635 27.626679203478034 22.02607594701025 17 21.870559855332015 28.421580572225192 27.568258223977043 22.139601348465753 18 21.751793730843794 27.761461143007416 28.945684224244868 21.541060901903926 19 24.005204254175215 26.896870348668738 27.06002697553931 22.037898421616738 20 23.555724156870596 27.315042159379445 27.67728932528702 21.451944358462946 21 23.21408 26.76933818181818 26.902807272727276 23.113774545454547 22 23.21003629440945 27.8035656726335 26.920351284268197 22.06604674868885 23 22.614169929772913 27.58801659384073 27.096019689185496 22.701793787200867 24 23.466089190711354 27.12422559729663 27.40303384331814 22.006651368673868 25 22.823241103407828 27.19072254920442 27.403202796454163 22.582833550933586 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 187.0 1 187.0 2 237.0 3 287.0 4 287.0 5 287.0 6 462.5 7 638.0 8 638.0 9 638.0 10 971.0 11 1304.0 12 1304.0 13 1304.0 14 2445.5 15 3587.0 16 3587.0 17 3587.0 18 6454.0 19 9321.0 20 9321.0 21 9321.0 22 14875.0 23 20429.0 24 20429.0 25 20429.0 26 29560.0 27 38691.0 28 38691.0 29 38691.0 30 50272.5 31 61854.0 32 61854.0 33 61854.0 34 71659.0 35 81464.0 36 81464.0 37 81464.0 38 88783.0 39 96102.0 40 96102.0 41 96102.0 42 101716.5 43 107331.0 44 107331.0 45 107331.0 46 111009.0 47 114687.0 48 114687.0 49 114687.0 50 110764.0 51 106841.0 52 106841.0 53 106841.0 54 95429.0 55 84017.0 56 84017.0 57 84017.0 58 71893.5 59 59770.0 60 59770.0 61 59770.0 62 48806.5 63 37843.0 64 37843.0 65 37843.0 66 29145.5 67 20448.0 68 20448.0 69 20448.0 70 14806.5 71 9165.0 72 9165.0 73 9165.0 74 6405.5 75 3646.0 76 3646.0 77 3646.0 78 2470.0 79 1294.0 80 1294.0 81 1294.0 82 855.0 83 416.0 84 416.0 85 416.0 86 269.0 87 122.0 88 122.0 89 122.0 90 79.0 91 36.0 92 36.0 93 36.0 94 23.0 95 10.0 96 10.0 97 10.0 98 9.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22664392470953973 2 0.1350788483510142 3 0.13775482898156832 4 0.0657360459244815 5 0.02175688599624432 6 0.020360722188998696 7 0.2907511128589014 8 0.2905184188910271 9 0.01745204759057031 10 0.0338569723257064 11 0.015357801879701873 12 0.01593953679938755 13 0.014543372992141925 14 0.016753965686947495 15 0.013961638072456248 16 0.018848211397815933 17 0.017568394574507446 18 0.013496250136707705 19 0.023385743771364216 20 0.016288577751198956 21 0.014310679024267655 22 0.01593953679938755 23 0.01663761870301036 24 0.014659719976079061 25 0.014427026008204788 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 859498.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.37432165573729 #Duplication Level Percentage of deduplicated Percentage of total 1 92.34142120051936 78.83586195721058 2 5.756445196453671 9.829052075913191 3 0.997368285089471 2.5544892234137877 4 0.3116811331822863 1.0643826127331681 5 0.16583591931133365 0.7079064558680348 6 0.0912681538893516 0.46751740362449 7 0.06226915190925591 0.37213306230315474 8 0.04882456008688853 0.3334690958046315 9 0.037585690608937114 0.28879675557303797 >10 0.16427283703947607 2.4496880158823076 >50 0.012810296944429426 0.7379541525359858 >100 0.008855231752254895 1.4294392588184217 >500 0.0012261088919883047 0.8035797632759927 >1k 1.3623432133203387E-4 0.12573016704318252 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1081 0.12577108963604336 No Hit GTCCTAAAGTGTGTATTTCTCATTT 930 0.10820269506153593 No Hit GTACATGGGAAGCAGTGGTATCAAC 870 0.1012218760253078 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 215 0.0 14.609593 4 CCTAGAC 85 3.7983136E-6 12.333743 3 TTGGGCC 70 1.0703183E-4 12.237906 4 GTCTAGC 55 0.0029725544 12.141241 1 GGACCTG 420 0.0 11.555322 6 GAACCAT 75 2.0393197E-4 11.419377 6 GTGTTAG 85 5.0958435E-5 11.222997 1 GTAGGAC 620 0.0 11.221536 3 AGATAGT 60 0.0057982416 11.102174 6 TGGGCCG 60 0.0057982416 11.102174 5 TGTAGGA 680 0.0 11.072337 2 CTAGACA 95 1.3293342E-5 11.021273 4 GTCCTAA 305 0.0 10.947021 1 GGCGAGG 235 0.0 10.897172 19 TGTTAGG 70 0.0014507383 10.892137 2 GCTGATT 70 0.0014800272 10.866078 8 GACGTGA 345 0.0 10.757384 7 TGGCGAG 565 0.0 10.743589 18 TAGACTT 80 3.7082922E-4 10.705667 5 GTGTTAT 90 9.110699E-5 10.599497 1 >>END_MODULE