FastQCFastQC Report
Thu 26 May 2016
SRR1926914_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926914_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859498
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13880.16148961370474393No Hit
GTCCTACAGTGGACATTTCTAAATT11350.13205382676864866No Hit
GTACATGGGAAGCAGTGGTATCAAC10110.11762680076044388No Hit
CTTTAGGACGTGAAATATGGCGAGG9910.11529986108170116No Hit
CTGTAGGACGTGGAATATGGCAAGA9180.1068065312542903No Hit
GTCCTAAAGTGTGTATTTCTCATTT9130.10622479633460463No Hit
GGTATCAACGCAGAGTACTTTTTTT8780.10215265189680486No Hit
CCCATGTACTCTGCGTTGATACCAC8650.10064014110562212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2800.014.9628384
GGCGAGG2400.012.651422519
GTAGGAC6100.012.0207333
GCCTTAC657.7913323E-411.732161
GGACCTG4850.011.5825526
GTATAGG752.007284E-411.4388571
TTAGGAC3950.011.3310673
TGTAGGA6550.011.2040412
TAAGACC855.193443E-511.2021254
GGACGTG4850.011.1899236
GTAGACT600.0057676311.110073
GTCCTAC6300.011.0454581
TAGAACC951.3240306E-511.02524954
TAAGAGT700.001461985210.8820654
GACGTGG1752.0008883E-1110.8453397
GTCCTAA3300.010.6878211
AGGACGT5000.010.6644235
AGGACCT5450.010.6574345
TAGGACT1355.482616E-810.5797854
TGGCGAG6300.010.54285318
Produced by FastQC (version 0.11.5)