##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926914_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 859498 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.916583866396433 31.0 30.0 33.0 26.0 34.0 2 30.239603815250298 31.0 30.0 34.0 26.0 34.0 3 30.298744150655384 31.0 30.0 34.0 26.0 34.0 4 33.91730521769684 37.0 35.0 37.0 30.0 37.0 5 33.88815913475075 37.0 35.0 37.0 30.0 37.0 6 33.83403917170255 37.0 35.0 37.0 30.0 37.0 7 33.82911420387249 37.0 35.0 37.0 29.0 37.0 8 33.84465816092649 37.0 35.0 37.0 30.0 37.0 9 35.394811855292275 39.0 35.0 39.0 30.0 39.0 10 35.28861614570366 39.0 35.0 39.0 30.0 39.0 11 35.31647892141692 39.0 35.0 39.0 30.0 39.0 12 35.215095323083936 39.0 35.0 39.0 29.0 39.0 13 35.23384347607557 39.0 35.0 39.0 29.0 39.0 14 36.34553076330602 40.0 36.0 41.0 29.0 41.0 15 36.32785416603645 40.0 36.0 41.0 29.0 41.0 16 36.29531424156892 40.0 36.0 41.0 29.0 41.0 17 36.269851704134275 40.0 36.0 41.0 29.0 41.0 18 36.23234259998278 40.0 36.0 41.0 29.0 41.0 19 36.300299709830625 40.0 37.0 41.0 29.0 41.0 20 36.263928479182034 40.0 37.0 41.0 29.0 41.0 21 36.221799236298395 40.0 37.0 41.0 29.0 41.0 22 36.13976646833384 40.0 37.0 41.0 29.0 41.0 23 36.06904379067782 40.0 37.0 41.0 29.0 41.0 24 36.00308901242353 40.0 36.0 41.0 29.0 41.0 25 35.89036158315668 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.12910816542203207 1101 2 -0.25197539318720885 1101 3 0.13412752569182373 1101 4 0.04199532832542019 1101 5 0.041828216481611946 1101 6 0.0036696950824719465 1101 7 0.11662062181530786 1101 8 0.05931034733535512 1101 9 0.10294357941762655 1101 10 -0.060229522783345146 1101 11 0.030068912937117886 1101 12 -0.08330152240007038 1101 13 -0.020928815560964154 1101 14 0.08781365369166849 1101 15 -0.040915117312962934 1101 16 -0.007034123356788768 1101 17 0.27702114561241586 1101 18 0.2735925790360625 1101 19 0.16316426890774238 1101 20 0.04690404441839746 1101 21 0.18143573753327757 1101 22 0.0957930518599639 1101 23 -0.033139292279521726 1101 24 0.06286224840534516 1101 25 0.06631167868683008 1102 1 -0.5328348734965687 1102 2 -0.5089629708269605 1102 3 -0.3006550830038286 1102 4 -0.28719721825843436 1102 5 -0.42478669035068606 1102 6 -0.39850421796100477 1102 7 -0.3468886949548775 1102 8 -0.24348468372054555 1102 9 -0.17112474356373752 1102 10 -0.06721710042310036 1102 11 -0.08017953426784885 1102 12 -0.13842574600255375 1102 13 -0.12263689009512291 1102 14 -0.17383230904125213 1102 15 -0.19541822290302235 1102 16 -0.037313626462378124 1102 17 -0.1732894133937961 1102 18 -0.31025835264095747 1102 19 -0.25220840189971483 1102 20 -0.33741272576794046 1102 21 -0.41716674693877565 1102 22 -0.17982806615246005 1102 23 -0.35301506867703836 1102 24 -0.3486284348244695 1102 25 -0.1922286939839779 1103 1 -0.6850231200326391 1103 2 -0.7051311504734024 1103 3 -0.5700148536679457 1103 4 -0.6039578489321045 1103 5 -0.545319418492106 1103 6 -0.47199203449230254 1103 7 -0.3953932338941968 1103 8 -0.4767885537635479 1103 9 -0.5701978443759685 1103 10 -0.5092381228789051 1103 11 -0.4285694052664155 1103 12 -0.7375896256012169 1103 13 -0.6460052608547997 1103 14 -0.6461065565328923 1103 15 -0.6952486099073241 1103 16 -0.7890097564193752 1103 17 -0.6913376694855273 1103 18 -0.6357886918669493 1103 19 -0.5075117941596261 1103 20 -0.5368178858252719 1103 21 -0.5600262787113479 1103 22 -0.5473125382021493 1103 23 -0.6527857327955289 1103 24 -0.600888348823517 1103 25 -0.6150906146719848 1104 1 -0.36930562461491334 1104 2 -0.1137056198165105 1104 3 0.019727947727428585 1104 4 0.021459285816312956 1104 5 -0.11438463459806059 1104 6 -0.27090647900244136 1104 7 -0.12287682639700392 1104 8 -0.1150472896471868 1104 9 -0.10581186909068663 1104 10 0.04512242817649792 1104 11 0.10648528639088539 1104 12 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2208 10 0.40765478813165146 2208 11 0.40910781946963226 2208 12 0.4513998163293991 2208 13 0.25114406041216597 2208 14 0.45422526850482114 2208 15 0.5212973931092648 2208 16 0.48972327525483195 2208 17 0.48686745871960113 2208 18 0.5031404182386368 2208 19 0.44022576240146094 2208 20 0.39067834343565977 2208 21 0.1440913683474747 2208 22 0.3585245658702476 2208 23 0.34716901833569125 2208 24 0.3375430237255017 2208 25 0.21530154219076536 2209 1 0.47979529575231794 2209 2 0.5625325612795287 2209 3 0.5411318435010557 2209 4 0.40648538812823176 2209 5 0.5335481823085786 2209 6 0.5150368474064706 2209 7 0.5610923251564159 2209 8 0.42294550400025344 2209 9 0.40339094601579717 2209 10 0.2647681682277607 2209 11 0.38836511003241014 2209 12 0.39738320825637174 2209 13 0.4100399207294174 2209 14 0.21479707515140944 2209 15 0.351457137876487 2209 16 0.2509959627684992 2209 17 0.28697231049731187 2209 18 0.29780867730663374 2209 19 0.13125000166536438 2209 20 0.21104313790935691 2209 21 0.2332736926927126 2209 22 0.3356242647718304 2209 23 0.33818652914327885 2209 24 0.41962065889738653 2209 25 0.3911004062030514 2210 1 -0.07023957270847347 2210 2 0.09325085157182045 2210 3 0.19940225802390543 2210 4 0.3399530702816165 2210 5 0.264793068725254 2210 6 0.18241314673710463 2210 7 0.008866509316803217 2210 8 0.14859606162107042 2210 9 0.2525291184691625 2210 10 0.23377971600304193 2210 11 0.23962284561416425 2210 12 0.13334549540775953 2210 13 0.12360841940880363 2210 14 0.019106487439387365 2210 15 0.2751967940240476 2210 16 0.15498524414874026 2210 17 0.18635236573773994 2210 18 0.029814080545278898 2210 19 0.04401076009557414 2210 20 0.1398790804285639 2210 21 0.2678807773050167 2210 22 0.25332261689386115 2210 23 0.24753127663521468 2210 24 0.1966755643681708 2210 25 0.1778136007580855 2211 1 -0.03627046075596496 2211 2 0.046616694112870505 2211 3 0.31855753464243364 2211 4 0.3075481414385166 2211 5 0.5011255484768782 2211 6 0.19853982084305244 2211 7 0.2518008093260491 2211 8 0.2468493367302429 2211 9 0.3717929741213979 2211 10 0.1810877084949638 2211 11 0.3413398994027119 2211 12 0.16394435715547218 2211 13 0.423300527970639 2211 14 0.33706889974300225 2211 15 0.378269473174079 2211 16 0.2873086849645645 2211 17 0.33290933532916256 2211 18 0.3213348105403995 2211 19 0.43672177430974557 2211 20 0.33183176147066007 2211 21 0.34291963999876174 2211 22 0.3252202128895547 2211 23 0.2406069443962764 2211 24 0.4200118863125297 2211 25 0.428952605572114 2212 1 0.49691481327868203 2212 2 0.46909796804756354 2212 3 0.4714927946208185 2212 4 0.47718224130669995 2212 5 0.5915354290923531 2212 6 0.5795145133232609 2212 7 0.4245616467044613 2212 8 0.2952341159844494 2212 9 0.19252252538302628 2212 10 0.3790748517001674 2212 11 0.3542654137057397 2212 12 0.32554452208228213 2212 13 0.43556071251374817 2212 14 0.38415425561633754 2212 15 0.34656166851239334 2212 16 0.45025512748070184 2212 17 0.43156868024485107 2212 18 0.3674455988780707 2212 19 0.24764942516468125 2212 20 0.28568348976622815 2212 21 0.29594048699790676 2212 22 0.4113569896948448 2212 23 0.39965511676978593 2212 24 0.26670491628048154 2212 25 0.4294180240426613 2213 1 0.25147260646308567 2213 2 0.37694862160637044 2213 3 0.2936318699787748 2213 4 0.3023384473641926 2213 5 0.404965481792793 2213 6 0.6837629512626862 2213 7 0.5693930908921274 2213 8 0.44489235791736803 2213 9 0.370884776706653 2213 10 0.3854912218504438 2213 11 0.5315730448760334 2213 12 0.41155567354407907 2213 13 0.3685816337320915 2213 14 0.3853464349201019 2213 15 0.4176858630271454 2213 16 0.5403349729253506 2213 17 0.5103717322238737 2213 18 0.47238794630969494 2213 19 0.22240273575490477 2213 20 0.31677185776447203 2213 21 0.3513982227131507 2213 22 0.13399800310839538 2213 23 0.33992081991102197 2213 24 0.2541258961907289 2213 25 0.3894677898864245 2214 1 0.3144786403429052 2214 2 0.16320129378137338 2214 3 0.20409375616881675 2214 4 0.27732669176990754 2214 5 0.2187262445826832 2214 6 0.2838662819485407 2214 7 0.18728787140826597 2214 8 0.16315916841718803 2214 9 0.26793935822725246 2214 10 0.04393588757665867 2214 11 0.12487986391100492 2214 12 0.22584790773922947 2214 13 0.10434254667747211 2214 14 0.1825416884260349 2214 15 -0.017080467370249153 2214 16 0.0700855172390007 2214 17 -0.0711893711818945 2214 18 -0.06448708085660115 2214 19 -0.013982507911897812 2214 20 0.06614061198759202 2214 21 0.11563039471231917 2214 22 0.13880396665221895 2214 23 0.12016529675744181 2214 24 0.0405050150941193 2214 25 0.11226988890211231 2215 1 0.12541024941481282 2215 2 0.21272367076170084 2215 3 0.08396131101244109 2215 4 0.13979079678836825 2215 5 0.1909752727321603 2215 6 0.03256917894215405 2215 7 0.05338249741674872 2215 8 0.11761014612811493 2215 9 -0.08432568836296639 2215 10 0.031444434000839294 2215 11 -0.14002119291539117 2215 12 0.06365631156020868 2215 13 -0.06219160971720328 2215 14 -0.09039691406267991 2215 15 -0.1521608525942142 2215 16 -0.21707042814202282 2215 17 -0.17898664023309152 2215 18 -0.12721881104354793 2215 19 -0.2901228450805391 2215 20 -0.20818299958474995 2215 21 -1.887922541428111E-4 2215 22 -0.03900267904722199 2215 23 -0.06479006649000496 2215 24 0.11171186786121012 2215 25 -0.025274796687149603 2216 1 -0.037880110370547726 2216 2 -0.04191757016058517 2216 3 -1.8933949849870082 2216 4 -1.354030981906 2216 5 -0.3738359401383988 2216 6 -0.010274210331729705 2216 7 -0.13676567824374075 2216 8 -0.11094592311175688 2216 9 -0.033468009325524406 2216 10 -0.039804503063606944 2216 11 -0.10520528846718236 2216 12 -0.026448857971899997 2216 13 -0.07238064119964349 2216 14 -0.2126564951438965 2216 15 -0.2437795446214679 2216 16 -0.11841241482967035 2216 17 -0.2431366338799137 2216 18 -0.25034535657628254 2216 19 -0.22126025943480698 2216 20 -0.06408901164601843 2216 21 0.037450366780568345 2216 22 -0.12816611210915596 2216 23 -0.13229942520998605 2216 24 -0.2835403533075791 2216 25 -0.16945724041265464 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19800.0 3 19.0 4 316.0 5 2172.0 6 2894.0 7 2071.0 8 1584.0 9 1377.0 10 1243.0 11 1159.0 12 1125.0 13 1037.0 14 1054.0 15 976.0 16 1035.0 17 1162.0 18 1308.0 19 1463.0 20 1925.0 21 2567.0 22 3140.0 23 3950.0 24 4655.0 25 5758.0 26 6886.0 27 7637.0 28 8787.0 29 10698.0 30 13840.0 31 17943.0 32 23051.0 33 30694.0 34 41118.0 35 57552.0 36 84143.0 37 145881.0 38 347478.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.297739264125234 19.53737027848632 11.021582398639598 28.14330805874885 2 14.300157241861278 20.738803797099763 39.771125735251296 25.189913225787663 3 17.713162598447187 25.210742147498554 29.46561563238936 27.610479621664897 4 12.115676825309658 17.868918834017066 37.65988984267561 32.35551449799767 5 13.228389883262146 38.36688009848193 35.12619160925572 13.278538409000207 6 31.570585777086734 37.73979366713592 17.950001984418808 12.739618571358532 7 28.26632624918978 31.873060277865378 21.812979075729313 18.04763439721553 8 25.47948648431655 37.22337094445136 19.731350265124057 17.565792306108037 9 26.45071130525037 15.68676504469366 19.766908771872664 38.095614878183305 10 14.807385541902326 29.62524273877991 33.789121812108256 21.778249907209503 11 34.00019314655518 22.86203634645838 24.304003369592913 18.833767137393522 12 23.280468834533846 25.115038749588418 31.24528064800993 20.3592117678678 13 28.688191759560578 22.077899412793357 25.65035433680088 23.583554490845184 14 21.240887122483123 22.18178518158274 27.253931946322158 29.32339574961198 15 23.139331406577963 30.525576048662206 23.173421945464444 23.161670599295384 16 22.471774156017016 28.224331468674663 27.168712827052133 22.135181548256188 17 21.8353800778364 28.614593453132365 27.45333015317134 22.096696315859894 18 21.83729245031664 27.80115719383404 28.764748520348626 21.596801835500695 19 23.468377434708906 27.02045498704475 27.220921073604675 22.29024650464167 20 23.689629896799264 27.4226576225436 27.413233429126578 21.474479051530558 21 23.159234720118036 26.73848438092711 26.917679585011832 23.184601313943016 22 23.289202870981516 27.837095417436014 26.670133810821913 22.20356790076056 23 22.564593011959317 27.61487241956633 27.107715326522374 22.71281924195198 24 23.080324004588746 27.077675934910538 27.625440084514842 22.21655997598587 25 22.7177423858813 27.274345632586193 27.411874488048255 22.59603749348425 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 177.0 1 177.0 2 195.0 3 213.0 4 213.0 5 213.0 6 361.0 7 509.0 8 509.0 9 509.0 10 858.0 11 1207.0 12 1207.0 13 1207.0 14 2239.0 15 3271.0 16 3271.0 17 3271.0 18 6171.5 19 9072.0 20 9072.0 21 9072.0 22 14414.0 23 19756.0 24 19756.0 25 19756.0 26 28716.0 27 37676.0 28 37676.0 29 37676.0 30 49261.5 31 60847.0 32 60847.0 33 60847.0 34 70544.0 35 80241.0 36 80241.0 37 80241.0 38 87778.5 39 95316.0 40 95316.0 41 95316.0 42 101037.0 43 106758.0 44 106758.0 45 106758.0 46 110762.5 47 114767.0 48 114767.0 49 114767.0 50 111844.5 51 108922.0 52 108922.0 53 108922.0 54 97072.0 55 85222.0 56 85222.0 57 85222.0 58 73012.5 59 60803.0 60 60803.0 61 60803.0 62 49735.0 63 38667.0 64 38667.0 65 38667.0 66 29863.5 67 21060.0 68 21060.0 69 21060.0 70 15268.0 71 9476.0 72 9476.0 73 9476.0 74 6588.0 75 3700.0 76 3700.0 77 3700.0 78 2489.0 79 1278.0 80 1278.0 81 1278.0 82 839.0 83 400.0 84 400.0 85 400.0 86 265.5 87 131.0 88 131.0 89 131.0 90 77.5 91 24.0 92 24.0 93 24.0 94 14.5 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10797000109366164 2 0.11029694077240436 3 0.017917435526318852 4 0.0 5 0.0059336961807939055 6 0.32856388263847036 7 0.0187318644138788 8 0.010354881570405051 9 0.010703922522216457 10 0.004072144437799739 11 0.005468308245045363 12 5.81734919685677E-4 13 5.81734919685677E-4 14 0.0 15 0.0025596336466169787 16 2.326939678742708E-4 17 3.4904095181140617E-4 18 5.81734919685677E-4 19 1.163469839371354E-4 20 9.307758714970832E-4 21 0.01163469839371354 22 1.163469839371354E-4 23 1.163469839371354E-4 24 4.653879357485416E-4 25 0.004886573325359686 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 859498.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.94529953195145 #Duplication Level Percentage of deduplicated Percentage of total 1 90.01090232492862 73.759703521575 2 7.465889762739433 12.235891457604257 3 1.3143790444345271 3.231215534841223 4 0.46195423737436686 1.5141991340678678 5 0.2193851921129704 0.8988792640286036 6 0.1298100236720871 0.6382392763235331 7 0.07640178951303546 0.43825372684959585 8 0.06187439745070591 0.4056252825965687 9 0.040483560388846956 0.2985693733965518 >10 0.19162451531162603 2.808302230882441 >50 0.015639758207830718 0.8837931080700026 >100 0.010091865532641655 1.6713167743959743 >500 0.0011371115151035964 0.8043844965454578 >1k 4.264168181638487E-4 0.41162681882300145 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1388 0.16148961370474393 No Hit GTCCTACAGTGGACATTTCTAAATT 1135 0.13205382676864866 No Hit GTACATGGGAAGCAGTGGTATCAAC 1011 0.11762680076044388 No Hit CTTTAGGACGTGAAATATGGCGAGG 991 0.11529986108170116 No Hit CTGTAGGACGTGGAATATGGCAAGA 918 0.1068065312542903 No Hit GTCCTAAAGTGTGTATTTCTCATTT 913 0.10622479633460463 No Hit GGTATCAACGCAGAGTACTTTTTTT 878 0.10215265189680486 No Hit CCCATGTACTCTGCGTTGATACCAC 865 0.10064014110562212 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 280 0.0 14.962838 4 GGCGAGG 240 0.0 12.6514225 19 GTAGGAC 610 0.0 12.020733 3 GCCTTAC 65 7.7913323E-4 11.73216 1 GGACCTG 485 0.0 11.582552 6 GTATAGG 75 2.007284E-4 11.438857 1 TTAGGAC 395 0.0 11.331067 3 TGTAGGA 655 0.0 11.204041 2 TAAGACC 85 5.193443E-5 11.202125 4 GGACGTG 485 0.0 11.189923 6 GTAGACT 60 0.00576763 11.11007 3 GTCCTAC 630 0.0 11.045458 1 TAGAACC 95 1.3240306E-5 11.0252495 4 TAAGAGT 70 0.0014619852 10.882065 4 GACGTGG 175 2.0008883E-11 10.845339 7 GTCCTAA 330 0.0 10.687821 1 AGGACGT 500 0.0 10.664423 5 AGGACCT 545 0.0 10.657434 5 TAGGACT 135 5.482616E-8 10.579785 4 TGGCGAG 630 0.0 10.542853 18 >>END_MODULE