FastQCFastQC Report
Thu 26 May 2016
SRR1926913_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926913_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597163
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10060.16846321691062574No Hit
GTATCAACGCAGAGTACTTTTTTTT8860.1483682009769527No Hit
GTCCTAAAGTGTGTATTTCTCATTT8830.1478658255786109No Hit
CTGTAGGACGTGGAATATGGCAAGA8360.13999527767125558No Hit
GTCCTACAGTGTGCATTTCTCATTT8070.13513898215395126No Hit
CTTTAGGACGTGAAATATGGCGAGG7210.12073755406815224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG1900.014.47360719
CCAACAA400.005338194314.22406213
GTACAAG1051.8768333E-812.7170891
TAGTATC550.003012508412.1186264
CTTGGAC657.780935E-411.7329223
ACTACAC657.780935E-411.7329223
GAGACCA658.1226707E-411.672989
TGGCGAG4750.011.57888618
GGGTATA600.005697398411.1274541
GCCTAGC600.005697398411.1274541
TTTATGA600.005718874711.1218332
GTTATAC600.005718874711.1218333
ACTTGTC600.00582001711.0956798
TTAGGAC3400.010.9349113
AGGGCTA700.001466017310.8774595
ACTGTTC1408.1545295E-910.8692368
ATGGCGA5500.010.86201117
GTGTAGC1855.456968E-1210.8267111
CTCTAGG803.6276164E-410.7300431
TAGGACG5250.010.70066454
Produced by FastQC (version 0.11.5)