##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926913_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 597163 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.93968480967508 31.0 28.0 31.0 16.0 34.0 2 28.17037894176297 31.0 28.0 31.0 16.0 34.0 3 27.634382907179447 31.0 28.0 31.0 16.0 34.0 4 31.31565753404012 35.0 32.0 37.0 17.0 37.0 5 31.229135093768367 35.0 32.0 37.0 17.0 37.0 6 30.960747735542892 35.0 32.0 37.0 11.0 37.0 7 31.096156325827288 35.0 32.0 37.0 13.0 37.0 8 30.689475067946272 35.0 31.0 37.0 11.0 37.0 9 32.86791043651398 37.0 33.0 39.0 17.0 39.0 10 33.03231613479067 37.0 33.0 39.0 17.0 39.0 11 33.18512868345829 37.0 33.0 39.0 17.0 39.0 12 33.06664679492869 38.0 33.0 39.0 17.0 39.0 13 33.17314703020783 38.0 33.0 39.0 17.0 39.0 14 34.16675514055626 39.0 34.0 40.0 17.0 41.0 15 33.71996423087164 39.0 33.0 40.0 10.0 41.0 16 33.79943499513533 39.0 33.0 40.0 11.0 41.0 17 34.02391641813039 39.0 34.0 41.0 16.0 41.0 18 34.06802330352014 39.0 34.0 41.0 11.0 41.0 19 33.45660230121424 39.0 33.0 40.0 10.0 41.0 20 33.743460328252084 39.0 33.0 41.0 10.0 41.0 21 33.733513295364915 39.0 34.0 41.0 8.0 41.0 22 33.753288465628316 39.0 34.0 41.0 2.0 41.0 23 33.73708016069315 39.0 34.0 41.0 2.0 41.0 24 32.43083881620261 38.0 32.0 40.0 2.0 41.0 25 32.99874741067347 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.04277170492779092 1101 2 -0.16196184557374238 1101 3 -0.9152532916932685 1101 4 -0.4884330347365875 1101 5 -0.393004552281333 1101 6 -1.9381795723540165 1101 7 -0.17538572229868876 1101 8 0.16278378744982902 1101 9 0.0424671287549927 1101 10 -8.890385271898282 1101 11 -3.783861705131116 1101 12 -1.315811923915728 1101 13 -0.5625471317274062 1101 14 -0.42587574580031884 1101 15 -1.705303041052936 1101 16 -0.1818412970811849 1101 17 0.008269077852922635 1101 18 0.1898639050101849 1101 19 -0.3395533649042477 1101 20 0.1567599986796253 1101 21 0.04821548582881974 1101 22 0.26715872718106226 1101 23 0.2599833099206279 1101 24 0.5081371808379416 1101 25 0.6304724383237499 1102 1 -0.03976737439626987 1102 2 -0.09288454831752802 1102 3 -0.2101786799530032 1102 4 -0.5464110439078809 1102 5 -0.2500476978819002 1102 6 -0.904281267269269 1102 7 -0.0675673239809349 1102 8 0.5131410117410802 1102 9 0.2370872295885107 1102 10 0.439456890702111 1102 11 -0.02940780226572315 1102 12 0.30685661754509397 1102 13 0.16643743922342225 1102 14 -0.25505159642482766 1102 15 0.5761149620379413 1102 16 0.6218525541006912 1102 17 0.1915770761301374 1102 18 0.10039568783808761 1102 19 0.5992130712134127 1102 20 0.46778201484339377 1102 21 0.23873637458258656 1102 22 0.3271787418753931 1102 23 0.011431208377722157 1102 24 -3.434927347549987 1102 25 -1.8458734865324757 1103 1 0.006511736987526717 1103 2 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-2.5895505682708624 2216 12 -2.1263933781443427 2216 13 -1.8463390837001796 2216 14 -1.9328257341675865 2216 15 -3.230953219649617 2216 16 -2.1701401351763394 2216 17 -2.4047100881484695 2216 18 -1.959340735310235 2216 19 -1.4916468008489367 2216 20 -2.4244285017925264 2216 21 -4.738094610928158 2216 22 -3.4597286268965526 2216 23 -3.499333141502447 2216 24 -3.6719671944386185 2216 25 -3.200290669030423 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37231.0 3 11.0 4 276.0 5 1598.0 6 2138.0 7 1709.0 8 1423.0 9 1256.0 10 1269.0 11 1125.0 12 1211.0 13 1105.0 14 1150.0 15 1187.0 16 1215.0 17 1381.0 18 1505.0 19 1923.0 20 2580.0 21 3342.0 22 4057.0 23 4857.0 24 5764.0 25 6534.0 26 7330.0 27 8010.0 28 9044.0 29 10674.0 30 12957.0 31 15355.0 32 19509.0 33 26034.0 34 34737.0 35 48511.0 36 69619.0 37 108828.0 38 140708.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.546324811570756 19.3579895510089 12.739176332179234 25.356509305241108 2 13.619115489415218 20.36034374345001 43.31754349193041 22.70299727520436 3 18.331298795374668 25.900115365009523 29.783623534461945 25.984962305153864 4 11.649540165165165 17.317038020163018 39.862518768768766 31.17090304590305 5 12.074337113281185 38.47774744141639 36.52953886869567 12.918376576606757 6 30.648967798668398 38.36774004438675 18.896696118253004 12.086596038691846 7 26.83634702398748 31.63165903586533 23.933227409077016 17.598766531070183 8 23.70964518496499 37.72053130725836 21.529901568092004 17.039921939684636 9 25.9079915314422 15.739189859170027 20.894357421377748 37.45846118801002 10 13.993171774434025 29.759571409904613 35.63157736031119 20.615679455350172 11 33.246294717554385 23.084215304168975 25.33510425372622 18.334385724550415 12 22.487417196358734 25.480993894932542 32.785468432027734 19.246120476680986 13 28.298110388675063 22.806312326858208 26.174349388832503 22.72122789563423 14 19.987672682933365 23.044880813602926 28.49871367126261 28.468732832201095 15 22.58365993367501 31.880882993334005 23.647204636049977 21.88825243694101 16 21.04972246136736 29.04809441338349 28.83621368297283 21.06596944227632 17 20.885402796132247 29.35721117045955 28.58205242873581 21.175333604672392 18 20.506851081718892 28.717932398640674 30.44975454687058 20.325461972769858 19 23.10562980407659 27.619388997670093 27.80816020930657 21.46682098894675 20 22.643275989997438 28.36359616579599 28.776628975608286 20.216498868598283 21 22.268058158601274 27.58932564227536 27.65380859783907 22.4888076012843 22 22.022239306189924 28.96888205530181 28.14851662588288 20.860362012625387 23 21.465252142220674 28.680968236846866 28.335767548120405 21.518012072812056 24 22.77137511912518 27.84589849278382 28.595068175613548 20.787658212477453 25 21.954724514953373 28.150793225426092 28.518095455032693 21.376386804587845 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 200.0 1 200.0 2 274.0 3 348.0 4 348.0 5 348.0 6 553.0 7 758.0 8 758.0 9 758.0 10 1100.0 11 1442.0 12 1442.0 13 1442.0 14 2487.0 15 3532.0 16 3532.0 17 3532.0 18 6010.5 19 8489.0 20 8489.0 21 8489.0 22 13116.0 23 17743.0 24 17743.0 25 17743.0 26 25149.5 27 32556.0 28 32556.0 29 32556.0 30 41843.5 31 51131.0 32 51131.0 33 51131.0 34 57171.0 35 63211.0 36 63211.0 37 63211.0 38 67752.5 39 72294.0 40 72294.0 41 72294.0 42 74374.0 43 76454.0 44 76454.0 45 76454.0 46 77392.5 47 78331.0 48 78331.0 49 78331.0 50 73408.5 51 68486.0 52 68486.0 53 68486.0 54 59211.0 55 49936.0 56 49936.0 57 49936.0 58 41843.5 59 33751.0 60 33751.0 61 33751.0 62 26954.0 63 20157.0 64 20157.0 65 20157.0 66 15394.5 67 10632.0 68 10632.0 69 10632.0 70 7750.0 71 4868.0 72 4868.0 73 4868.0 74 3334.5 75 1801.0 76 1801.0 77 1801.0 78 1257.5 79 714.0 80 714.0 81 714.0 82 471.0 83 228.0 84 228.0 85 228.0 86 146.0 87 64.0 88 64.0 89 64.0 90 45.5 91 27.0 92 27.0 93 27.0 94 17.5 95 8.0 96 8.0 97 8.0 98 5.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22037534140594778 2 0.13195727129778637 3 0.13312948056058396 4 0.07150476503065327 5 0.02562114531543314 6 0.023109268323724008 7 0.2962340265555636 8 0.28903331251266406 9 0.021937059060926415 10 0.03834798874009274 11 0.018420431272533632 12 0.019760099001445167 13 0.018252972806419687 14 0.01925772360310334 15 0.017248222009736035 16 0.02160214212869853 17 0.02076484979812882 18 0.017415680475849977 19 0.024114019120407663 20 0.019425182069217284 21 0.01775059740807786 22 0.018755348204761515 23 0.019927557467559108 24 0.017080763543622094 25 0.0179180558741918 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 597163.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.4342751639469 #Duplication Level Percentage of deduplicated Percentage of total 1 94.43998818386467 82.57291913347918 2 4.263360961529678 7.455277508672302 3 0.633626035595539 1.6620189944190351 4 0.2174866977903369 0.7606316711639393 5 0.11021450798025592 0.4818262808902358 6 0.07405235079889708 0.3884828169772733 7 0.04849521726278877 0.29681009192030433 8 0.026494248620656313 0.18532043393283879 9 0.023943128027695174 0.1884105039833198 >10 0.13588257582170712 2.2368399185546837 >50 0.01284467398882994 0.7579838808472134 >100 0.012269447859486096 2.066256131875701 >500 0.001150260736692219 0.778596554875944 >1k 1.9171012278203646E-4 0.16862607840800856 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1006 0.16846321691062574 No Hit GTATCAACGCAGAGTACTTTTTTTT 886 0.1483682009769527 No Hit GTCCTAAAGTGTGTATTTCTCATTT 883 0.1478658255786109 No Hit CTGTAGGACGTGGAATATGGCAAGA 836 0.13999527767125558 No Hit GTCCTACAGTGTGCATTTCTCATTT 807 0.13513898215395126 No Hit CTTTAGGACGTGAAATATGGCGAGG 721 0.12073755406815224 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGAGG 190 0.0 14.473607 19 CCAACAA 40 0.0053381943 14.224062 13 GTACAAG 105 1.8768333E-8 12.717089 1 TAGTATC 55 0.0030125084 12.118626 4 CTTGGAC 65 7.780935E-4 11.732922 3 ACTACAC 65 7.780935E-4 11.732922 3 GAGACCA 65 8.1226707E-4 11.67298 9 TGGCGAG 475 0.0 11.578886 18 GGGTATA 60 0.0056973984 11.127454 1 GCCTAGC 60 0.0056973984 11.127454 1 TTTATGA 60 0.0057188747 11.121833 2 GTTATAC 60 0.0057188747 11.121833 3 ACTTGTC 60 0.005820017 11.095679 8 TTAGGAC 340 0.0 10.934911 3 AGGGCTA 70 0.0014660173 10.877459 5 ACTGTTC 140 8.1545295E-9 10.869236 8 ATGGCGA 550 0.0 10.862011 17 GTGTAGC 185 5.456968E-12 10.826711 1 CTCTAGG 80 3.6276164E-4 10.730043 1 TAGGACG 525 0.0 10.7006645 4 >>END_MODULE