FastQCFastQC Report
Thu 26 May 2016
SRR1926913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926913_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597163
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14140.2367862710851141No Hit
GTCCTACAGTGGACATTTCTAAATT10960.18353447886088053No Hit
CTGTAGGACGTGGAATATGGCAAGA10430.1746591801568416No Hit
CTTTAGGACGTGAAATATGGCGAGG9370.15690858274876374No Hit
GGTATCAACGCAGAGTACTTTTTTT8950.14987532717197816No Hit
GTCCTAAAGTGTGTATTTCTCATTT8840.1480332840447248No Hit
TATCAACGCAGAGTACTTTTTTTTT7920.13262710516224213No Hit
GTCCTACAGTGTGCATTTCTCATTT7750.1297803112383051No Hit
CTGTAGGACCTGGAATATGGCGAGA6400.10717341831292294No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAAT350.002185086516.2666918
TTGGCGA350.002187532816.26396817
TAGGACC2400.013.8884
TGTACTG852.6108683E-713.4445155
TAACACT500.001470374913.3324794
TTAGGAC2800.013.2644563
ATAATAC957.125709E-813.0317463
GGACGTG5500.012.986186
TCCAACG957.486415E-812.9826418
AGGACGT5800.012.6428685
GACGTGA2650.012.5367137
GTTCTAA853.783056E-612.3365831
GTAGGAC6950.012.1951763
GTTTAGA550.00298647612.1326731
TAGGACG5950.012.0040324
GACGTGG3100.011.9416517
TTAGTAC657.8484084E-411.72086053
TAGGACA1950.011.72086054
CTAGGTC657.8484084E-411.72086054
TGTAGGA7200.011.5188172
Produced by FastQC (version 0.11.5)